A universal method for generating knockout mice in multiple genetic backgrounds using zygote electroporation

Bibliographic Details
Main Authors: Tomohiro Tamari, Yoshihisa Ikeda, Kento Morimoto, Keiko Kobayashi, Saori Mizuno-Iijima, Shinya Ayabe, Akihiro Kuno, Seiya Mizuno, Atsushi Yoshiki
Format: Article
Language:English
Published: The Company of Biologists 2023-09-01
Series:Biology Open
Subjects:
Online Access:http://bio.biologists.org/content/12/9/bio059970
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author Tomohiro Tamari
Yoshihisa Ikeda
Kento Morimoto
Keiko Kobayashi
Saori Mizuno-Iijima
Shinya Ayabe
Akihiro Kuno
Seiya Mizuno
Atsushi Yoshiki
author_facet Tomohiro Tamari
Yoshihisa Ikeda
Kento Morimoto
Keiko Kobayashi
Saori Mizuno-Iijima
Shinya Ayabe
Akihiro Kuno
Seiya Mizuno
Atsushi Yoshiki
author_sort Tomohiro Tamari
collection DOAJ
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spelling doaj.art-df15e47e773446f9b99e32c76716beb42023-10-11T08:06:00ZengThe Company of BiologistsBiology Open2046-63902023-09-0112910.1242/bio.059970059970A universal method for generating knockout mice in multiple genetic backgrounds using zygote electroporationTomohiro Tamari0Yoshihisa Ikeda1Kento Morimoto2Keiko Kobayashi3Saori Mizuno-Iijima4Shinya Ayabe5Akihiro Kuno6Seiya Mizuno7Atsushi Yoshiki8 Model Generation & Breeding Service, The Jackson Laboratory Japan, Inc., 955 Kamibayashi, Ishioka, Ibaraki 315-0138, Japan Model Generation & Breeding Service, The Jackson Laboratory Japan, Inc., 955 Kamibayashi, Ishioka, Ibaraki 315-0138, Japan Doctoral Program in Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan Model Generation & Breeding Service, The Jackson Laboratory Japan, Inc., 955 Kamibayashi, Ishioka, Ibaraki 315-0138, Japan Experimental Animal Division, RIKEN BioResource Research Center, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan Experimental Animal Division, RIKEN BioResource Research Center, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan Department of Anatomy and Embryology, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan Laboratory Animal Resource Center in Trans-Border Medical Research Center, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan Experimental Animal Division, RIKEN BioResource Research Center, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan http://bio.biologists.org/content/12/9/bio059970mousegenome editingknockoutelectroporationin vitro fertilisationlong-read sequencing
spellingShingle Tomohiro Tamari
Yoshihisa Ikeda
Kento Morimoto
Keiko Kobayashi
Saori Mizuno-Iijima
Shinya Ayabe
Akihiro Kuno
Seiya Mizuno
Atsushi Yoshiki
A universal method for generating knockout mice in multiple genetic backgrounds using zygote electroporation
Biology Open
mouse
genome editing
knockout
electroporation
in vitro fertilisation
long-read sequencing
title A universal method for generating knockout mice in multiple genetic backgrounds using zygote electroporation
title_full A universal method for generating knockout mice in multiple genetic backgrounds using zygote electroporation
title_fullStr A universal method for generating knockout mice in multiple genetic backgrounds using zygote electroporation
title_full_unstemmed A universal method for generating knockout mice in multiple genetic backgrounds using zygote electroporation
title_short A universal method for generating knockout mice in multiple genetic backgrounds using zygote electroporation
title_sort universal method for generating knockout mice in multiple genetic backgrounds using zygote electroporation
topic mouse
genome editing
knockout
electroporation
in vitro fertilisation
long-read sequencing
url http://bio.biologists.org/content/12/9/bio059970
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