Resveratrol Modulates the Redox Response and Bile Acid Metabolism to Maintain the Cholesterol Homeostasis in Fish <i>Megalobrama amblycephala</i> Offered a High-Carbohydrate Diet
This study aimed to characterize the effects of resveratrol on the redox balance, cholesterol homeostasis and bile acid metabolism of <i>Megalobrama amblycephala</i> offered a high-carbohydrate diet. Fish (35.0 ± 0.15 g) were fed four diets including one control diet (32% nitrogen-free e...
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MDPI AG
2023-01-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/1/121 |
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| author | Yaping Ge Ling Zhang Weiliang Chen Miao Sun Wenbin Liu Xiangfei Li |
| author_facet | Yaping Ge Ling Zhang Weiliang Chen Miao Sun Wenbin Liu Xiangfei Li |
| author_sort | Yaping Ge |
| collection | DOAJ |
| description | This study aimed to characterize the effects of resveratrol on the redox balance, cholesterol homeostasis and bile acid metabolism of <i>Megalobrama amblycephala</i> offered a high-carbohydrate diet. Fish (35.0 ± 0.15 g) were fed four diets including one control diet (32% nitrogen-free extract), one high-carbohydrate diet (45% nitrogen-free extract, HC), and the HC diet supplemented with different levels (0.04%, HCR1; 0.08%, HCR2) of resveratrol for 12 weeks. The HC diet-induced redox imbalance is characterized by increased MDA content and decreased T-SOD and CAT activities in the liver. Resveratrol attenuated this by up-regulating the transcription of <i>Cu/Zn-sod</i>, and increasing the activities of T-SOD, CAT, and GPX. The HC diet enhanced the cholesterol synthesis, but decreased the bile acid synthesis via up-regulating both <i>hmgcr</i> and <i>acat2</i>, and down-regulating <i>cyp7a1</i>, thus resulting in excessive cholesterol accumulation. Resveratrol supplement decreased cholesterol synthesis, and increased cholesterol uptake in the liver by down-regulating both <i>hmgcr</i> and <i>acat2</i>, and up-regulating <i>ldlr</i>. It also increased bile acid synthesis and biliary excretion by up-regulating <i>cyp7a1</i>, and down-regulating <i>mrp2</i>, <i>oatp1</i>, and <i>oatp4</i> in the hindgut, thereby decreasing cholesterol accumulation. In conclusion, resveratrol improves the cholesterol homeostasis of <i>Megalobrama amblycephala</i> fed a high-carbohydrate diet by modulating the redox response and bile acid metabolism. |
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| last_indexed | 2024-03-09T13:46:40Z |
| publishDate | 2023-01-01 |
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| series | Antioxidants |
| spelling | doaj.art-df1d5ff1992343da9d03a9b1cc9fdb102023-11-30T20:58:37ZengMDPI AGAntioxidants2076-39212023-01-0112112110.3390/antiox12010121Resveratrol Modulates the Redox Response and Bile Acid Metabolism to Maintain the Cholesterol Homeostasis in Fish <i>Megalobrama amblycephala</i> Offered a High-Carbohydrate DietYaping Ge0Ling Zhang1Weiliang Chen2Miao Sun3Wenbin Liu4Xiangfei Li5Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, ChinaKey Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, ChinaKey Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, ChinaKey Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, ChinaKey Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, ChinaKey Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, ChinaThis study aimed to characterize the effects of resveratrol on the redox balance, cholesterol homeostasis and bile acid metabolism of <i>Megalobrama amblycephala</i> offered a high-carbohydrate diet. Fish (35.0 ± 0.15 g) were fed four diets including one control diet (32% nitrogen-free extract), one high-carbohydrate diet (45% nitrogen-free extract, HC), and the HC diet supplemented with different levels (0.04%, HCR1; 0.08%, HCR2) of resveratrol for 12 weeks. The HC diet-induced redox imbalance is characterized by increased MDA content and decreased T-SOD and CAT activities in the liver. Resveratrol attenuated this by up-regulating the transcription of <i>Cu/Zn-sod</i>, and increasing the activities of T-SOD, CAT, and GPX. The HC diet enhanced the cholesterol synthesis, but decreased the bile acid synthesis via up-regulating both <i>hmgcr</i> and <i>acat2</i>, and down-regulating <i>cyp7a1</i>, thus resulting in excessive cholesterol accumulation. Resveratrol supplement decreased cholesterol synthesis, and increased cholesterol uptake in the liver by down-regulating both <i>hmgcr</i> and <i>acat2</i>, and up-regulating <i>ldlr</i>. It also increased bile acid synthesis and biliary excretion by up-regulating <i>cyp7a1</i>, and down-regulating <i>mrp2</i>, <i>oatp1</i>, and <i>oatp4</i> in the hindgut, thereby decreasing cholesterol accumulation. In conclusion, resveratrol improves the cholesterol homeostasis of <i>Megalobrama amblycephala</i> fed a high-carbohydrate diet by modulating the redox response and bile acid metabolism.https://www.mdpi.com/2076-3921/12/1/121redox balancecholesterol homeostasisbile acid metabolismcarbohydrate utilizationresveratrolfish culture |
| spellingShingle | Yaping Ge Ling Zhang Weiliang Chen Miao Sun Wenbin Liu Xiangfei Li Resveratrol Modulates the Redox Response and Bile Acid Metabolism to Maintain the Cholesterol Homeostasis in Fish <i>Megalobrama amblycephala</i> Offered a High-Carbohydrate Diet Antioxidants redox balance cholesterol homeostasis bile acid metabolism carbohydrate utilization resveratrol fish culture |
| title | Resveratrol Modulates the Redox Response and Bile Acid Metabolism to Maintain the Cholesterol Homeostasis in Fish <i>Megalobrama amblycephala</i> Offered a High-Carbohydrate Diet |
| title_full | Resveratrol Modulates the Redox Response and Bile Acid Metabolism to Maintain the Cholesterol Homeostasis in Fish <i>Megalobrama amblycephala</i> Offered a High-Carbohydrate Diet |
| title_fullStr | Resveratrol Modulates the Redox Response and Bile Acid Metabolism to Maintain the Cholesterol Homeostasis in Fish <i>Megalobrama amblycephala</i> Offered a High-Carbohydrate Diet |
| title_full_unstemmed | Resveratrol Modulates the Redox Response and Bile Acid Metabolism to Maintain the Cholesterol Homeostasis in Fish <i>Megalobrama amblycephala</i> Offered a High-Carbohydrate Diet |
| title_short | Resveratrol Modulates the Redox Response and Bile Acid Metabolism to Maintain the Cholesterol Homeostasis in Fish <i>Megalobrama amblycephala</i> Offered a High-Carbohydrate Diet |
| title_sort | resveratrol modulates the redox response and bile acid metabolism to maintain the cholesterol homeostasis in fish i megalobrama amblycephala i offered a high carbohydrate diet |
| topic | redox balance cholesterol homeostasis bile acid metabolism carbohydrate utilization resveratrol fish culture |
| url | https://www.mdpi.com/2076-3921/12/1/121 |
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