Electrosprayed Mesenchymal Stromal Cell Extracellular Matrix Nanoparticles Accelerate Cellular Wound Healing and Reduce Gram-Negative Bacterial Growth
Treatments for acute respiratory distress syndrome are still unavailable, and the prevalence of the disease has only increased due to the COVID-19 pandemic. Mechanical ventilation regimens are still utilized to support declining lung function but also contribute to lung damage and increase the risk...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | Pharmaceutics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4923/15/4/1277 |
| _version_ | 1797603843209428992 |
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| author | Emily N. Wandling Keera Rhoads Dennis E. Ohman Rebecca L. Heise |
| author_facet | Emily N. Wandling Keera Rhoads Dennis E. Ohman Rebecca L. Heise |
| author_sort | Emily N. Wandling |
| collection | DOAJ |
| description | Treatments for acute respiratory distress syndrome are still unavailable, and the prevalence of the disease has only increased due to the COVID-19 pandemic. Mechanical ventilation regimens are still utilized to support declining lung function but also contribute to lung damage and increase the risk for bacterial infection. The anti-inflammatory and pro-regenerative abilities of mesenchymal stromal cells (MSCs) have shown to be a promising therapy for ARDS. We propose to utilize the regenerative effects of MSCs and the extracellular matrix (ECM) in a nanoparticle. Our mouse MSC (MMSC) ECM nanoparticles were characterized using size, zeta potential, and mass spectrometry to evaluate their potential as pro-regenerative and antimicrobial treatments. The nanoparticles had an average size of 273.4 nm (±25.6) and possessed a negative zeta potential, allowing them to surpass defenses and reach the distal regions of the lung. It was found that the MMSC ECM nanoparticles are biocompatible with mouse lung epithelial cells and MMSCs, increasing the wound healing rate of human lung fibroblasts while also inhibiting the growth of <i>Pseudomonas aeruginosa</i>, a common lung pathogen. Our MMSC ECM nanoparticles display characteristics of healing injured lungs while preventing bacterial infection, which can increase recovery time. |
| first_indexed | 2024-03-11T04:38:42Z |
| format | Article |
| id | doaj.art-df1d9b33390140e298fa8eddb3ca65ac |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-11T04:38:42Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-df1d9b33390140e298fa8eddb3ca65ac2023-11-17T20:55:13ZengMDPI AGPharmaceutics1999-49232023-04-01154127710.3390/pharmaceutics15041277Electrosprayed Mesenchymal Stromal Cell Extracellular Matrix Nanoparticles Accelerate Cellular Wound Healing and Reduce Gram-Negative Bacterial GrowthEmily N. Wandling0Keera Rhoads1Dennis E. Ohman2Rebecca L. Heise3Department of Biomedical Engineering, Virginia Commonwealth University, Richmond, VA 23219, USADepartment of Biomedical Engineering, Virginia Commonwealth University, Richmond, VA 23219, USADepartment of Microbiology and Immunology, Virginia Commonwealth University, Richmond, VA 23298, USADepartment of Biomedical Engineering, Virginia Commonwealth University, Richmond, VA 23219, USATreatments for acute respiratory distress syndrome are still unavailable, and the prevalence of the disease has only increased due to the COVID-19 pandemic. Mechanical ventilation regimens are still utilized to support declining lung function but also contribute to lung damage and increase the risk for bacterial infection. The anti-inflammatory and pro-regenerative abilities of mesenchymal stromal cells (MSCs) have shown to be a promising therapy for ARDS. We propose to utilize the regenerative effects of MSCs and the extracellular matrix (ECM) in a nanoparticle. Our mouse MSC (MMSC) ECM nanoparticles were characterized using size, zeta potential, and mass spectrometry to evaluate their potential as pro-regenerative and antimicrobial treatments. The nanoparticles had an average size of 273.4 nm (±25.6) and possessed a negative zeta potential, allowing them to surpass defenses and reach the distal regions of the lung. It was found that the MMSC ECM nanoparticles are biocompatible with mouse lung epithelial cells and MMSCs, increasing the wound healing rate of human lung fibroblasts while also inhibiting the growth of <i>Pseudomonas aeruginosa</i>, a common lung pathogen. Our MMSC ECM nanoparticles display characteristics of healing injured lungs while preventing bacterial infection, which can increase recovery time.https://www.mdpi.com/1999-4923/15/4/1277mesenchymal stromal cellsextracellular matrixnanoparticlesacute respiratory distress syndromeventilator-associated pneumoniaantimicrobial |
| spellingShingle | Emily N. Wandling Keera Rhoads Dennis E. Ohman Rebecca L. Heise Electrosprayed Mesenchymal Stromal Cell Extracellular Matrix Nanoparticles Accelerate Cellular Wound Healing and Reduce Gram-Negative Bacterial Growth Pharmaceutics mesenchymal stromal cells extracellular matrix nanoparticles acute respiratory distress syndrome ventilator-associated pneumonia antimicrobial |
| title | Electrosprayed Mesenchymal Stromal Cell Extracellular Matrix Nanoparticles Accelerate Cellular Wound Healing and Reduce Gram-Negative Bacterial Growth |
| title_full | Electrosprayed Mesenchymal Stromal Cell Extracellular Matrix Nanoparticles Accelerate Cellular Wound Healing and Reduce Gram-Negative Bacterial Growth |
| title_fullStr | Electrosprayed Mesenchymal Stromal Cell Extracellular Matrix Nanoparticles Accelerate Cellular Wound Healing and Reduce Gram-Negative Bacterial Growth |
| title_full_unstemmed | Electrosprayed Mesenchymal Stromal Cell Extracellular Matrix Nanoparticles Accelerate Cellular Wound Healing and Reduce Gram-Negative Bacterial Growth |
| title_short | Electrosprayed Mesenchymal Stromal Cell Extracellular Matrix Nanoparticles Accelerate Cellular Wound Healing and Reduce Gram-Negative Bacterial Growth |
| title_sort | electrosprayed mesenchymal stromal cell extracellular matrix nanoparticles accelerate cellular wound healing and reduce gram negative bacterial growth |
| topic | mesenchymal stromal cells extracellular matrix nanoparticles acute respiratory distress syndrome ventilator-associated pneumonia antimicrobial |
| url | https://www.mdpi.com/1999-4923/15/4/1277 |
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