Matrix metalloproteinases and their inhibitors in canine mammary tumors
<p>Abstract</p> <p>Background</p> <p>Malignant canine mammary tumors represent 50% of all neoplasms in female dogs. Matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) are thought to be involved in tumor progression, and they are also as...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2011-07-01
|
| Series: | BMC Veterinary Research |
| Online Access: | http://www.biomedcentral.com/1746-6148/7/33 |
| _version_ | 1818359787951226880 |
|---|---|
| author | Bradaschia Alice Aricò Arianna Garbisa Spiridione Granato Anna Zancanella Vanessa Lopparelli Rosa Castagnaro Massimo Vascellari Marta Morello Emanuela Giantin Mery Aresu Luca Mutinelli Franco Dacasto Mauro |
| author_facet | Bradaschia Alice Aricò Arianna Garbisa Spiridione Granato Anna Zancanella Vanessa Lopparelli Rosa Castagnaro Massimo Vascellari Marta Morello Emanuela Giantin Mery Aresu Luca Mutinelli Franco Dacasto Mauro |
| author_sort | Bradaschia Alice |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Malignant canine mammary tumors represent 50% of all neoplasms in female dogs. Matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) are thought to be involved in tumor progression, and they are also associated with the reactive stroma, which provides structural and vascular support for tumor growth.</p> <p>Results</p> <p>MMP-2, MMP-9 and MT1-MMP were expressed at both the mRNA and protein levels in tumor samples. MMP-2 and MMP-9 immunohistochemical reactions were evident both in the epithelial tumor cells and in the stromal compartment to varying degrees; in particular, the intensity of the MMP-2 staining was stronger in the stromal fibroblasts close to epithelial tumor cells in simple carcinomas than in adenomas. These data were supported by gelatin-zymography; bands for the active form of MMP-2 were found in 94% of carcinoma samples, compared with 17% of benign tumor samples. The gene expression and immunohistochemical results for MT1-MMP were comparable to those for MMP-2. The immunoreactivity for MMP-13 and TIMP-2 was lower in carcinomas than in adenomas, confirming the mRNA data for MMP-13 and the other MMP inhibitors that were evaluated. The active form of MMP-9, but not the active form of MMP-2, was identified in the plasma of all of the tested dogs.</p> <p>Conclusions</p> <p>Our findings suggest that MMP-9, MMP-2 and MT1-MMP, which are synthesized by epithelial cancer cells and cancer-associated fibroblasts, play an important role in malignant canine mammary tumors. The reduction of MMP-13 and TIMP-2 could also be a significant step in malignant transformation. MMP-2 and MT1-MMP could be further evaluated as future biomarkers for predicting the progression and prognosis of canine mammary tumors.</p> |
| first_indexed | 2024-12-13T20:50:27Z |
| format | Article |
| id | doaj.art-df1f8bd44cf34d55a15a39f35ad75c5c |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-12-13T20:50:27Z |
| publishDate | 2011-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-df1f8bd44cf34d55a15a39f35ad75c5c2022-12-21T23:31:53ZengBMCBMC Veterinary Research1746-61482011-07-01713310.1186/1746-6148-7-33Matrix metalloproteinases and their inhibitors in canine mammary tumorsBradaschia AliceAricò AriannaGarbisa SpiridioneGranato AnnaZancanella VanessaLopparelli RosaCastagnaro MassimoVascellari MartaMorello EmanuelaGiantin MeryAresu LucaMutinelli FrancoDacasto Mauro<p>Abstract</p> <p>Background</p> <p>Malignant canine mammary tumors represent 50% of all neoplasms in female dogs. Matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) are thought to be involved in tumor progression, and they are also associated with the reactive stroma, which provides structural and vascular support for tumor growth.</p> <p>Results</p> <p>MMP-2, MMP-9 and MT1-MMP were expressed at both the mRNA and protein levels in tumor samples. MMP-2 and MMP-9 immunohistochemical reactions were evident both in the epithelial tumor cells and in the stromal compartment to varying degrees; in particular, the intensity of the MMP-2 staining was stronger in the stromal fibroblasts close to epithelial tumor cells in simple carcinomas than in adenomas. These data were supported by gelatin-zymography; bands for the active form of MMP-2 were found in 94% of carcinoma samples, compared with 17% of benign tumor samples. The gene expression and immunohistochemical results for MT1-MMP were comparable to those for MMP-2. The immunoreactivity for MMP-13 and TIMP-2 was lower in carcinomas than in adenomas, confirming the mRNA data for MMP-13 and the other MMP inhibitors that were evaluated. The active form of MMP-9, but not the active form of MMP-2, was identified in the plasma of all of the tested dogs.</p> <p>Conclusions</p> <p>Our findings suggest that MMP-9, MMP-2 and MT1-MMP, which are synthesized by epithelial cancer cells and cancer-associated fibroblasts, play an important role in malignant canine mammary tumors. The reduction of MMP-13 and TIMP-2 could also be a significant step in malignant transformation. MMP-2 and MT1-MMP could be further evaluated as future biomarkers for predicting the progression and prognosis of canine mammary tumors.</p>http://www.biomedcentral.com/1746-6148/7/33 |
| spellingShingle | Bradaschia Alice Aricò Arianna Garbisa Spiridione Granato Anna Zancanella Vanessa Lopparelli Rosa Castagnaro Massimo Vascellari Marta Morello Emanuela Giantin Mery Aresu Luca Mutinelli Franco Dacasto Mauro Matrix metalloproteinases and their inhibitors in canine mammary tumors BMC Veterinary Research |
| title | Matrix metalloproteinases and their inhibitors in canine mammary tumors |
| title_full | Matrix metalloproteinases and their inhibitors in canine mammary tumors |
| title_fullStr | Matrix metalloproteinases and their inhibitors in canine mammary tumors |
| title_full_unstemmed | Matrix metalloproteinases and their inhibitors in canine mammary tumors |
| title_short | Matrix metalloproteinases and their inhibitors in canine mammary tumors |
| title_sort | matrix metalloproteinases and their inhibitors in canine mammary tumors |
| url | http://www.biomedcentral.com/1746-6148/7/33 |
| work_keys_str_mv | AT bradaschiaalice matrixmetalloproteinasesandtheirinhibitorsincaninemammarytumors AT aricoarianna matrixmetalloproteinasesandtheirinhibitorsincaninemammarytumors AT garbisaspiridione matrixmetalloproteinasesandtheirinhibitorsincaninemammarytumors AT granatoanna matrixmetalloproteinasesandtheirinhibitorsincaninemammarytumors AT zancanellavanessa matrixmetalloproteinasesandtheirinhibitorsincaninemammarytumors AT lopparellirosa matrixmetalloproteinasesandtheirinhibitorsincaninemammarytumors AT castagnaromassimo matrixmetalloproteinasesandtheirinhibitorsincaninemammarytumors AT vascellarimarta matrixmetalloproteinasesandtheirinhibitorsincaninemammarytumors AT morelloemanuela matrixmetalloproteinasesandtheirinhibitorsincaninemammarytumors AT giantinmery matrixmetalloproteinasesandtheirinhibitorsincaninemammarytumors AT aresuluca matrixmetalloproteinasesandtheirinhibitorsincaninemammarytumors AT mutinellifranco matrixmetalloproteinasesandtheirinhibitorsincaninemammarytumors AT dacastomauro matrixmetalloproteinasesandtheirinhibitorsincaninemammarytumors |