Detection of Antimicrobial Susceptibility and Integrons Among Extended-spectrum β-lactamase Producing Uropathogenic Escherichia coli Isolates in Southwestern Iran
Objectives: Urinary tract infections (UTIs) are one of the most prevalent infectious diseases and can lead to a high rate of morbidity and mortality. The emergence of multiple-drug resistant strains, particularly extended-spectrum beta-lactamases (ESBLs) producing strains, has become a global health...
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| Format: | Article |
| Language: | English |
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Oman Medical Specialty Board
2018-05-01
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| Series: | Oman Medical Journal |
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| Online Access: | http://omjournal.org/articleDetails.aspx?coType=1&aId=2122 |
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| author | Hadi Sedigh Ebrahim-Saraie Niloofar Zahedian Nezhad Hamid Heidari Ashkan Motamedifar Mohammad Motamedifar |
| author_facet | Hadi Sedigh Ebrahim-Saraie Niloofar Zahedian Nezhad Hamid Heidari Ashkan Motamedifar Mohammad Motamedifar |
| author_sort | Hadi Sedigh Ebrahim-Saraie |
| collection | DOAJ |
| description | Objectives: Urinary tract infections (UTIs) are one of the most prevalent infectious diseases and can lead to a high rate of morbidity and mortality. The emergence of multiple-drug resistant strains, particularly extended-spectrum beta-lactamases (ESBLs) producing strains, has become a global healthcare concern. Our study sought to investigate the antimicrobial resistance pattern and presence of integrons and fimH determinants among uropathogenic Escherichia coli (UPEC) isolates obtained from hospitalized Iranian patients.Methods: This cross-sectional study was performed on 121 E. coli isolates recovered from patients with clinical symptoms of UTIs, referred to Shiraz Nemazee Hospital, in 2016–17. The isolates were identified by standard microbiologic tests and confirmed by API 20E strip. Antimicrobial susceptibility testing was determined using the disk diffusion method. The presence of fimH and classes 1–3 integron encoding genes was determined using the polymerase chain reaction.Results: Ampicillin (9.1%) and nalidixic acid (19.0%) showed the lowest level of antibiotic susceptibility. The highest level of susceptibility was toward imipenem (77.7%). The rate of ESBL-producing isolates was 42.1%. There was a significant association between production of ESBLs and higher antibiotic resistance in the tested isolates. Of the investigated virulence and resistance genes, fimH, intI1, and intI2 were positive in 98.3%, 59.5%, and 7.4% of isolates, respectively.Conclusions: The remarkable rate of ESBL-producing UPEC isolates accompanied with the presence of integrons suggest the necessity of restricted infection control policies to prevent further dissemination of resistant strains. |
| first_indexed | 2024-12-22T21:14:50Z |
| format | Article |
| id | doaj.art-df264d8b52004baeb4e2f8824ae34b14 |
| institution | Directory Open Access Journal |
| issn | 1999-768X 2070-5204 |
| language | English |
| last_indexed | 2024-12-22T21:14:50Z |
| publishDate | 2018-05-01 |
| publisher | Oman Medical Specialty Board |
| record_format | Article |
| series | Oman Medical Journal |
| spelling | doaj.art-df264d8b52004baeb4e2f8824ae34b142022-12-21T18:12:25ZengOman Medical Specialty BoardOman Medical Journal1999-768X2070-52042018-05-0133321822310.5001/omj.2018.40Detection of Antimicrobial Susceptibility and Integrons Among Extended-spectrum β-lactamase Producing Uropathogenic Escherichia coli Isolates in Southwestern IranHadi Sedigh Ebrahim-Saraie0Niloofar Zahedian Nezhad1Hamid Heidari2Ashkan Motamedifar3Mohammad Motamedifar4Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranStudent Research Committee, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranStudent Research Committee, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; Shiraz HIV/AIDS Research Center, Institute of Health, Shiraz University of Medical Sciences, Shiraz, IranObjectives: Urinary tract infections (UTIs) are one of the most prevalent infectious diseases and can lead to a high rate of morbidity and mortality. The emergence of multiple-drug resistant strains, particularly extended-spectrum beta-lactamases (ESBLs) producing strains, has become a global healthcare concern. Our study sought to investigate the antimicrobial resistance pattern and presence of integrons and fimH determinants among uropathogenic Escherichia coli (UPEC) isolates obtained from hospitalized Iranian patients.Methods: This cross-sectional study was performed on 121 E. coli isolates recovered from patients with clinical symptoms of UTIs, referred to Shiraz Nemazee Hospital, in 2016–17. The isolates were identified by standard microbiologic tests and confirmed by API 20E strip. Antimicrobial susceptibility testing was determined using the disk diffusion method. The presence of fimH and classes 1–3 integron encoding genes was determined using the polymerase chain reaction.Results: Ampicillin (9.1%) and nalidixic acid (19.0%) showed the lowest level of antibiotic susceptibility. The highest level of susceptibility was toward imipenem (77.7%). The rate of ESBL-producing isolates was 42.1%. There was a significant association between production of ESBLs and higher antibiotic resistance in the tested isolates. Of the investigated virulence and resistance genes, fimH, intI1, and intI2 were positive in 98.3%, 59.5%, and 7.4% of isolates, respectively.Conclusions: The remarkable rate of ESBL-producing UPEC isolates accompanied with the presence of integrons suggest the necessity of restricted infection control policies to prevent further dissemination of resistant strains.http://omjournal.org/articleDetails.aspx?coType=1&aId=2122Urinary Tract InfectionsEscherichia coliAntibiotic ResistanceIntegronsExtended-Spectrum Beta-lactamases |
| spellingShingle | Hadi Sedigh Ebrahim-Saraie Niloofar Zahedian Nezhad Hamid Heidari Ashkan Motamedifar Mohammad Motamedifar Detection of Antimicrobial Susceptibility and Integrons Among Extended-spectrum β-lactamase Producing Uropathogenic Escherichia coli Isolates in Southwestern Iran Oman Medical Journal Urinary Tract Infections Escherichia coli Antibiotic Resistance Integrons Extended-Spectrum Beta-lactamases |
| title | Detection of Antimicrobial Susceptibility and Integrons Among Extended-spectrum β-lactamase Producing Uropathogenic Escherichia coli Isolates in Southwestern Iran |
| title_full | Detection of Antimicrobial Susceptibility and Integrons Among Extended-spectrum β-lactamase Producing Uropathogenic Escherichia coli Isolates in Southwestern Iran |
| title_fullStr | Detection of Antimicrobial Susceptibility and Integrons Among Extended-spectrum β-lactamase Producing Uropathogenic Escherichia coli Isolates in Southwestern Iran |
| title_full_unstemmed | Detection of Antimicrobial Susceptibility and Integrons Among Extended-spectrum β-lactamase Producing Uropathogenic Escherichia coli Isolates in Southwestern Iran |
| title_short | Detection of Antimicrobial Susceptibility and Integrons Among Extended-spectrum β-lactamase Producing Uropathogenic Escherichia coli Isolates in Southwestern Iran |
| title_sort | detection of antimicrobial susceptibility and integrons among extended spectrum β lactamase producing uropathogenic escherichia coli isolates in southwestern iran |
| topic | Urinary Tract Infections Escherichia coli Antibiotic Resistance Integrons Extended-Spectrum Beta-lactamases |
| url | http://omjournal.org/articleDetails.aspx?coType=1&aId=2122 |
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