Foreskin-derived mesenchymal stromal cells with aldehyde dehydrogenase activity: isolation and gene profiling
Abstract Background Mesenchymal stromal cells (MSCs) become an attractive research topic because of their crucial roles in tissue repair and regenerative medicine. Foreskin is considered as a valuable tissue source containing immunotherapeutic MSCs (FSK-MSCs). Results In this work, we used aldehyde...
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| Format: | Article |
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BMC
2018-04-01
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| Series: | BMC Cell Biology |
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| Online Access: | http://link.springer.com/article/10.1186/s12860-018-0157-0 |
| _version_ | 1818297537583382528 |
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| author | Mehdi Najar Emerence Crompot Leo A. van Grunsven Laurent Dollé Laurence Lagneaux |
| author_facet | Mehdi Najar Emerence Crompot Leo A. van Grunsven Laurent Dollé Laurence Lagneaux |
| author_sort | Mehdi Najar |
| collection | DOAJ |
| description | Abstract Background Mesenchymal stromal cells (MSCs) become an attractive research topic because of their crucial roles in tissue repair and regenerative medicine. Foreskin is considered as a valuable tissue source containing immunotherapeutic MSCs (FSK-MSCs). Results In this work, we used aldehyde dehydrogenase activity (ALDH) assay (ALDEFLUOR™) to isolate and therefore characterize subsets of FSK-MSCs. According to their ALDH activity, we were able to distinguish and sort by fluorescence activated cell sorting (FACS) two subsets of FSK-MSCs (referred as ALDH+ and ALDH−). Consequently, these subsets were characterized by profiling the gene expression related to the main properties of MSCs (proliferation, response to hypoxia, angiogenesis, phenotype, stemness, multilineage, hematopoiesis and immunomodulation). We thus demonstrated by Real Time PCR several relevant differences in gene expression based on their ALDH activity. Conclusion Taken together, this preliminary study suggests that distinct subsets of FSK-MSCs with differential gene expression profiles depending of ALDH activity could be identified. These populations could differ in terms of biological functionalities involving the selection by ALDH activity as useful tool for potent therapeutic applications. However, functional studies should be conducted to confirm their therapeutic relevance. |
| first_indexed | 2024-12-13T04:21:01Z |
| format | Article |
| id | doaj.art-df26dc312ac94f55b8cf854fe7331bb4 |
| institution | Directory Open Access Journal |
| issn | 1471-2121 |
| language | English |
| last_indexed | 2024-12-13T04:21:01Z |
| publishDate | 2018-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cell Biology |
| spelling | doaj.art-df26dc312ac94f55b8cf854fe7331bb42022-12-21T23:59:46ZengBMCBMC Cell Biology1471-21212018-04-0119111110.1186/s12860-018-0157-0Foreskin-derived mesenchymal stromal cells with aldehyde dehydrogenase activity: isolation and gene profilingMehdi Najar0Emerence Crompot1Leo A. van Grunsven2Laurent Dollé3Laurence Lagneaux4Laboratory of Clinical Cell Therapy, Jules Bordet Institute, Université Libre de Bruxelles (ULB)Laboratory of Clinical Cell Therapy, Jules Bordet Institute, Université Libre de Bruxelles (ULB)Liver Cell Biology Laboratory, Vrije Universiteit BrusselLiver Cell Biology Laboratory, Vrije Universiteit BrusselLaboratory of Clinical Cell Therapy, Jules Bordet Institute, Université Libre de Bruxelles (ULB)Abstract Background Mesenchymal stromal cells (MSCs) become an attractive research topic because of their crucial roles in tissue repair and regenerative medicine. Foreskin is considered as a valuable tissue source containing immunotherapeutic MSCs (FSK-MSCs). Results In this work, we used aldehyde dehydrogenase activity (ALDH) assay (ALDEFLUOR™) to isolate and therefore characterize subsets of FSK-MSCs. According to their ALDH activity, we were able to distinguish and sort by fluorescence activated cell sorting (FACS) two subsets of FSK-MSCs (referred as ALDH+ and ALDH−). Consequently, these subsets were characterized by profiling the gene expression related to the main properties of MSCs (proliferation, response to hypoxia, angiogenesis, phenotype, stemness, multilineage, hematopoiesis and immunomodulation). We thus demonstrated by Real Time PCR several relevant differences in gene expression based on their ALDH activity. Conclusion Taken together, this preliminary study suggests that distinct subsets of FSK-MSCs with differential gene expression profiles depending of ALDH activity could be identified. These populations could differ in terms of biological functionalities involving the selection by ALDH activity as useful tool for potent therapeutic applications. However, functional studies should be conducted to confirm their therapeutic relevance.http://link.springer.com/article/10.1186/s12860-018-0157-0Foreskin mesenchymal stromal cellsAldehyde dehydrogenase activityFluorescence activated cell sortingTranscriptome analysis |
| spellingShingle | Mehdi Najar Emerence Crompot Leo A. van Grunsven Laurent Dollé Laurence Lagneaux Foreskin-derived mesenchymal stromal cells with aldehyde dehydrogenase activity: isolation and gene profiling BMC Cell Biology Foreskin mesenchymal stromal cells Aldehyde dehydrogenase activity Fluorescence activated cell sorting Transcriptome analysis |
| title | Foreskin-derived mesenchymal stromal cells with aldehyde dehydrogenase activity: isolation and gene profiling |
| title_full | Foreskin-derived mesenchymal stromal cells with aldehyde dehydrogenase activity: isolation and gene profiling |
| title_fullStr | Foreskin-derived mesenchymal stromal cells with aldehyde dehydrogenase activity: isolation and gene profiling |
| title_full_unstemmed | Foreskin-derived mesenchymal stromal cells with aldehyde dehydrogenase activity: isolation and gene profiling |
| title_short | Foreskin-derived mesenchymal stromal cells with aldehyde dehydrogenase activity: isolation and gene profiling |
| title_sort | foreskin derived mesenchymal stromal cells with aldehyde dehydrogenase activity isolation and gene profiling |
| topic | Foreskin mesenchymal stromal cells Aldehyde dehydrogenase activity Fluorescence activated cell sorting Transcriptome analysis |
| url | http://link.springer.com/article/10.1186/s12860-018-0157-0 |
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