Single‐cell RNA sequencing analysis of human kidney reveals the presence of ACE2 receptor: A potential pathway of COVID‐19 infection
Abstract Background A novel coronavirus called SARS‐Cov‐2, which shared 82% similarity of genome sequence with SARS‐CoV, was found in Wuhan in late December of 2019, causing an epidemic outbreak of novel coronavirus‐induced pneumonia with dramatically increasing number of cases. Several organs are v...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-10-01
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| Series: | Molecular Genetics & Genomic Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/mgg3.1442 |
| _version_ | 1797301025574486016 |
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| author | Qiyu He Tsz N. Mok Liang Yun Chengbo He Jieruo Li Jinghua Pan |
| author_facet | Qiyu He Tsz N. Mok Liang Yun Chengbo He Jieruo Li Jinghua Pan |
| author_sort | Qiyu He |
| collection | DOAJ |
| description | Abstract Background A novel coronavirus called SARS‐Cov‐2, which shared 82% similarity of genome sequence with SARS‐CoV, was found in Wuhan in late December of 2019, causing an epidemic outbreak of novel coronavirus‐induced pneumonia with dramatically increasing number of cases. Several organs are vulnerable to COVID‐19 infection. Acute kidney injury (AKI) was reported in parts of case‐studies reporting characteristics of COVID‐19 patients. This study aimed at analyzing the potential route of SARS‐Cov‐2 entry and mechanism at cellular level. Method Single‐cell RNA sequencing (scRNA‐seq) technology was used to obtain evidence of potential route and ACE2 expressing cell in renal system for underlying pathogenesis of kidney injury caused by COVID‐19. The whole process was performed under R with Seurat packages. Canonical marker genes were used to annotate different types of cells. Results Ten different clusters were identified and ACE2 was mainly expressed in proximal tubule and glomerular parietal epithelial cells. From Gene Ontology (GO) & KEGG enrichment analysis, imbalance of ACE2 expression, renin‐angiotensin system (RAS) activation, and neutrophil‐related processes were the main issue of COVID‐19 leading kidney injury. Conclusion Our study provided the cellular evidence that SARS‐Cov‐2 invaded human kidney tissue via proximal convoluted tubule, proximal tubule, proximal straight tubule cells, and glomerular parietal cells by means of ACE2‐related pathway and used their cellular protease TMPRSS2 for priming. |
| first_indexed | 2024-03-07T23:15:29Z |
| format | Article |
| id | doaj.art-df2af63a63584084b1bfa68505f9fc77 |
| institution | Directory Open Access Journal |
| issn | 2324-9269 |
| language | English |
| last_indexed | 2024-03-07T23:15:29Z |
| publishDate | 2020-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | Molecular Genetics & Genomic Medicine |
| spelling | doaj.art-df2af63a63584084b1bfa68505f9fc772024-02-21T12:03:03ZengWileyMolecular Genetics & Genomic Medicine2324-92692020-10-01810n/an/a10.1002/mgg3.1442Single‐cell RNA sequencing analysis of human kidney reveals the presence of ACE2 receptor: A potential pathway of COVID‐19 infectionQiyu He0Tsz N. Mok1Liang Yun2Chengbo He3Jieruo Li4Jinghua Pan5First Affiliated Hospital of Jinan University Guangzhou Guangdong ChinaFirst Affiliated Hospital of Jinan University Guangzhou Guangdong ChinaFirst Medical College of Southern Medical University Guangzhou Guangdong ChinaHeyu Health Technology Co Ltd. Guangzhou Guangdong ChinaFirst Affiliated Hospital of Jinan University Guangzhou Guangdong ChinaFirst Affiliated Hospital of Jinan University Guangzhou Guangdong ChinaAbstract Background A novel coronavirus called SARS‐Cov‐2, which shared 82% similarity of genome sequence with SARS‐CoV, was found in Wuhan in late December of 2019, causing an epidemic outbreak of novel coronavirus‐induced pneumonia with dramatically increasing number of cases. Several organs are vulnerable to COVID‐19 infection. Acute kidney injury (AKI) was reported in parts of case‐studies reporting characteristics of COVID‐19 patients. This study aimed at analyzing the potential route of SARS‐Cov‐2 entry and mechanism at cellular level. Method Single‐cell RNA sequencing (scRNA‐seq) technology was used to obtain evidence of potential route and ACE2 expressing cell in renal system for underlying pathogenesis of kidney injury caused by COVID‐19. The whole process was performed under R with Seurat packages. Canonical marker genes were used to annotate different types of cells. Results Ten different clusters were identified and ACE2 was mainly expressed in proximal tubule and glomerular parietal epithelial cells. From Gene Ontology (GO) & KEGG enrichment analysis, imbalance of ACE2 expression, renin‐angiotensin system (RAS) activation, and neutrophil‐related processes were the main issue of COVID‐19 leading kidney injury. Conclusion Our study provided the cellular evidence that SARS‐Cov‐2 invaded human kidney tissue via proximal convoluted tubule, proximal tubule, proximal straight tubule cells, and glomerular parietal cells by means of ACE2‐related pathway and used their cellular protease TMPRSS2 for priming.https://doi.org/10.1002/mgg3.1442ACE2COVID‐19kidneySARS‐Cov‐2scRNA‐seq |
| spellingShingle | Qiyu He Tsz N. Mok Liang Yun Chengbo He Jieruo Li Jinghua Pan Single‐cell RNA sequencing analysis of human kidney reveals the presence of ACE2 receptor: A potential pathway of COVID‐19 infection Molecular Genetics & Genomic Medicine ACE2 COVID‐19 kidney SARS‐Cov‐2 scRNA‐seq |
| title | Single‐cell RNA sequencing analysis of human kidney reveals the presence of ACE2 receptor: A potential pathway of COVID‐19 infection |
| title_full | Single‐cell RNA sequencing analysis of human kidney reveals the presence of ACE2 receptor: A potential pathway of COVID‐19 infection |
| title_fullStr | Single‐cell RNA sequencing analysis of human kidney reveals the presence of ACE2 receptor: A potential pathway of COVID‐19 infection |
| title_full_unstemmed | Single‐cell RNA sequencing analysis of human kidney reveals the presence of ACE2 receptor: A potential pathway of COVID‐19 infection |
| title_short | Single‐cell RNA sequencing analysis of human kidney reveals the presence of ACE2 receptor: A potential pathway of COVID‐19 infection |
| title_sort | single cell rna sequencing analysis of human kidney reveals the presence of ace2 receptor a potential pathway of covid 19 infection |
| topic | ACE2 COVID‐19 kidney SARS‐Cov‐2 scRNA‐seq |
| url | https://doi.org/10.1002/mgg3.1442 |
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