Organic extracts in PM2.5 are the major triggers to induce ferroptosis in SH-SY5Y cells
As a major air pollutant, PM2.5 can induce apoptosis of nerve cells, causing impairment of the learning and memory capabilities of humans and animals. Ferroptosis is a newly discovered way of programmed cell death. It is unclear whether the neurotoxicity induced by PM2.5 is related to the ferroptosi...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651322011903 |
| _version_ | 1828071709511516160 |
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| author | CanCan Guo Yi Lyu ShuangShuang Xia XueKe Ren ZhaoFei Li FengJie Tian JinPing Zheng |
| author_facet | CanCan Guo Yi Lyu ShuangShuang Xia XueKe Ren ZhaoFei Li FengJie Tian JinPing Zheng |
| author_sort | CanCan Guo |
| collection | DOAJ |
| description | As a major air pollutant, PM2.5 can induce apoptosis of nerve cells, causing impairment of the learning and memory capabilities of humans and animals. Ferroptosis is a newly discovered way of programmed cell death. It is unclear whether the neurotoxicity induced by PM2.5 is related to the ferroptosis of nerve cells. In this study, we observed the changes in ferroptosis hallmarks of SH-SY5Y cells after exposure to various doses (40, 80, and 160 μg/mL PM2.5) for 24 h, exposure to 40 μg/mL PM2.5 for various times (24, 48, and 72 h), as well as exposure to various components (Po, organic extracts; Pw, water-soluble extracts; Pc, carbon core component). The results showed that PM2.5 reduced the cell viability, the content of GSH, and the activity of GSH-PX and SOD in SH-SY5Y cells with exposure dose and duration increasing. On the other hand, PM2.5 increased the content of iron, MDA, and the level of lipid ROS in SH-SY5Y cells with exposure dose and duration increasing. Additionally, PM2.5 reduced the expression levels of HO-1, NRF2, SLC7A11, and GPX4. The ferroptosis inhibitors Fer-1 and DFO significantly increase the cells viabilities and significantly reversed the changes of other above ferroptosis hallmarks. We also observed the different effects on ferroptosis hallmarks in the SH-SY5Y cells exposed to PM2.5 (160 μg/mL) and its various components (organic extracts, water-soluble extracts, and carbon core) for 24 h. We found that only the organic extracts shared similar results with PM2.5 (160 μg/mL). This study demonstrated that PM2.5 induced ferroptosis of SH-SY5Y cells, and organic extracts might be the primary component that caused ferroptosis. |
| first_indexed | 2024-04-11T00:57:03Z |
| format | Article |
| id | doaj.art-df2e3b18645d45be9dffedb92affb9ad |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-04-11T00:57:03Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-df2e3b18645d45be9dffedb92affb9ad2023-01-05T04:30:19ZengElsevierEcotoxicology and Environmental Safety0147-65132023-01-01249114350Organic extracts in PM2.5 are the major triggers to induce ferroptosis in SH-SY5Y cellsCanCan Guo0Yi Lyu1ShuangShuang Xia2XueKe Ren3ZhaoFei Li4FengJie Tian5JinPing Zheng6Department of Health Toxicology, School of Public Health in Shanxi Medical University, Taiyuan 030001, ChinaDepartment of Health Toxicology, School of Public Health in Shanxi Medical University, Taiyuan 030001, China; Department of Biochemistry and Molecular Biology, School of Preclinical Medicine in Shanxi Medical University, Taiyuan 030001, ChinaDepartment of Health Toxicology, School of Public Health in Shanxi Medical University, Taiyuan 030001, ChinaDepartment of Health Toxicology, School of Public Health in Shanxi Medical University, Taiyuan 030001, ChinaDepartment of Health Toxicology, School of Public Health in Shanxi Medical University, Taiyuan 030001, ChinaDepartment of Health Toxicology, School of Public Health in Shanxi Medical University, Taiyuan 030001, ChinaDepartment of Health Toxicology, School of Public Health in Shanxi Medical University, Taiyuan 030001, China; Collaborative Innovation Center for Aging Mechanism Research and Transformation, Center for Healthy Aging, Changzhi Medical College, Changzhi 046000, China; Corresponding author at: Department of Health Toxicology, School of Public Health in Shanxi Medical University, Taiyuan 030001, China.As a major air pollutant, PM2.5 can induce apoptosis of nerve cells, causing impairment of the learning and memory capabilities of humans and animals. Ferroptosis is a newly discovered way of programmed cell death. It is unclear whether the neurotoxicity induced by PM2.5 is related to the ferroptosis of nerve cells. In this study, we observed the changes in ferroptosis hallmarks of SH-SY5Y cells after exposure to various doses (40, 80, and 160 μg/mL PM2.5) for 24 h, exposure to 40 μg/mL PM2.5 for various times (24, 48, and 72 h), as well as exposure to various components (Po, organic extracts; Pw, water-soluble extracts; Pc, carbon core component). The results showed that PM2.5 reduced the cell viability, the content of GSH, and the activity of GSH-PX and SOD in SH-SY5Y cells with exposure dose and duration increasing. On the other hand, PM2.5 increased the content of iron, MDA, and the level of lipid ROS in SH-SY5Y cells with exposure dose and duration increasing. Additionally, PM2.5 reduced the expression levels of HO-1, NRF2, SLC7A11, and GPX4. The ferroptosis inhibitors Fer-1 and DFO significantly increase the cells viabilities and significantly reversed the changes of other above ferroptosis hallmarks. We also observed the different effects on ferroptosis hallmarks in the SH-SY5Y cells exposed to PM2.5 (160 μg/mL) and its various components (organic extracts, water-soluble extracts, and carbon core) for 24 h. We found that only the organic extracts shared similar results with PM2.5 (160 μg/mL). This study demonstrated that PM2.5 induced ferroptosis of SH-SY5Y cells, and organic extracts might be the primary component that caused ferroptosis.http://www.sciencedirect.com/science/article/pii/S0147651322011903PM2.5FerroptosisSH-SY5Y cells |
| spellingShingle | CanCan Guo Yi Lyu ShuangShuang Xia XueKe Ren ZhaoFei Li FengJie Tian JinPing Zheng Organic extracts in PM2.5 are the major triggers to induce ferroptosis in SH-SY5Y cells Ecotoxicology and Environmental Safety PM2.5 Ferroptosis SH-SY5Y cells |
| title | Organic extracts in PM2.5 are the major triggers to induce ferroptosis in SH-SY5Y cells |
| title_full | Organic extracts in PM2.5 are the major triggers to induce ferroptosis in SH-SY5Y cells |
| title_fullStr | Organic extracts in PM2.5 are the major triggers to induce ferroptosis in SH-SY5Y cells |
| title_full_unstemmed | Organic extracts in PM2.5 are the major triggers to induce ferroptosis in SH-SY5Y cells |
| title_short | Organic extracts in PM2.5 are the major triggers to induce ferroptosis in SH-SY5Y cells |
| title_sort | organic extracts in pm2 5 are the major triggers to induce ferroptosis in sh sy5y cells |
| topic | PM2.5 Ferroptosis SH-SY5Y cells |
| url | http://www.sciencedirect.com/science/article/pii/S0147651322011903 |
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