Late Male-Killing Viruses in Homona magnanima Identified as Osugoroshi Viruses, Novel Members of Partitiviridae
Late male-killing, a male-specific death after hatching, is a unique phenotype found in Homona magnanima, oriental tea tortrix. The male-killing agent was suspected to be an RNA virus, but details were unknown. We herein successfully isolated and identified the putative male-killing virus as Osugoro...
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Frontiers Media S.A.
2021-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2020.620623/full |
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| author | Ryosuke Fujita Ryosuke Fujita Maki N. Inoue Takumi Takamatsu Hiroshi Arai Mayu Nishino Nobuhiko Abe Kentaro Itokawa Madoka Nakai Syun-ichi Urayama Yuto Chiba Michael Amoa-Bosompem Yasuhisa Kunimi |
| author_facet | Ryosuke Fujita Ryosuke Fujita Maki N. Inoue Takumi Takamatsu Hiroshi Arai Mayu Nishino Nobuhiko Abe Kentaro Itokawa Madoka Nakai Syun-ichi Urayama Yuto Chiba Michael Amoa-Bosompem Yasuhisa Kunimi |
| author_sort | Ryosuke Fujita |
| collection | DOAJ |
| description | Late male-killing, a male-specific death after hatching, is a unique phenotype found in Homona magnanima, oriental tea tortrix. The male-killing agent was suspected to be an RNA virus, but details were unknown. We herein successfully isolated and identified the putative male-killing virus as Osugoroshi viruses (OGVs). The three RNA-dependent RNA polymerase genes detected were phylogenetically related to Partitiviridae, a group of segmented double-stranded RNA viruses. Purified dsRNA from a late male-killing strain of H. magnanima revealed 24 segments, in addition to the RdRps, with consensus terminal sequences. These segments included the previously found male-killing agents MK1068 (herein OGV-related RNA16) and MK1241 (OGV-related RNA7) RNAs. Ultramicroscopic observation of purified virions, which induced late male-killing in the progeny of injected moths, showed sizes typical of Partitiviridae. Mathematical modeling showed the importance of late male-killing in facilitating horizontal transmission of OGVs in an H. magnanima population. This study is the first report on the isolation of partiti-like virus from insects, and one thought to be associated with late male-killing, although the viral genomic contents and combinations in each virus are still unknown. |
| first_indexed | 2024-12-16T15:41:18Z |
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| id | doaj.art-df2efe7e59104658a1aecd83a12b6bb4 |
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| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-16T15:41:18Z |
| publishDate | 2021-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-df2efe7e59104658a1aecd83a12b6bb42022-12-21T22:25:59ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-01-011110.3389/fmicb.2020.620623620623Late Male-Killing Viruses in Homona magnanima Identified as Osugoroshi Viruses, Novel Members of PartitiviridaeRyosuke Fujita0Ryosuke Fujita1Maki N. Inoue2Takumi Takamatsu3Hiroshi Arai4Mayu Nishino5Nobuhiko Abe6Kentaro Itokawa7Madoka Nakai8Syun-ichi Urayama9Yuto Chiba10Michael Amoa-Bosompem11Yasuhisa Kunimi12Laboratory of Sanitary Entomology, Faculty of Agriculture, Kyushu University, Fukuoka, JapanDepartment of Medical Entomology, National Institute of Infectious Diseases, Tokyo, JapanDepartment of Applied Biological Science, Tokyo University of Agriculture and Technology, Fuchu, JapanDepartment of Applied Biological Science, Tokyo University of Agriculture and Technology, Fuchu, JapanDepartment of Applied Biological Science, Tokyo University of Agriculture and Technology, Fuchu, JapanDepartment of Applied Biological Science, Tokyo University of Agriculture and Technology, Fuchu, JapanDepartment of Applied Biological Science, Tokyo University of Agriculture and Technology, Fuchu, JapanPathogen Genomics Center, Natinal Institute of Infectious Diseases, Tokyo, JapanDepartment of Applied Biological Science, Tokyo University of Agriculture and Technology, Fuchu, JapanDepartment of Life and Environmental Sciences, University of Tsukuba, Tsukuba, JapanDepartment of Life and Environmental Sciences, University of Tsukuba, Tsukuba, JapanLaboratory of Sanitary Entomology, Faculty of Agriculture, Kyushu University, Fukuoka, JapanDepartment of Applied Biological Science, Tokyo University of Agriculture and Technology, Fuchu, JapanLate male-killing, a male-specific death after hatching, is a unique phenotype found in Homona magnanima, oriental tea tortrix. The male-killing agent was suspected to be an RNA virus, but details were unknown. We herein successfully isolated and identified the putative male-killing virus as Osugoroshi viruses (OGVs). The three RNA-dependent RNA polymerase genes detected were phylogenetically related to Partitiviridae, a group of segmented double-stranded RNA viruses. Purified dsRNA from a late male-killing strain of H. magnanima revealed 24 segments, in addition to the RdRps, with consensus terminal sequences. These segments included the previously found male-killing agents MK1068 (herein OGV-related RNA16) and MK1241 (OGV-related RNA7) RNAs. Ultramicroscopic observation of purified virions, which induced late male-killing in the progeny of injected moths, showed sizes typical of Partitiviridae. Mathematical modeling showed the importance of late male-killing in facilitating horizontal transmission of OGVs in an H. magnanima population. This study is the first report on the isolation of partiti-like virus from insects, and one thought to be associated with late male-killing, although the viral genomic contents and combinations in each virus are still unknown.https://www.frontiersin.org/articles/10.3389/fmicb.2020.620623/fullmale killingHomona magnanimaPartitiviridaehorizontal transmissioninsect virus |
| spellingShingle | Ryosuke Fujita Ryosuke Fujita Maki N. Inoue Takumi Takamatsu Hiroshi Arai Mayu Nishino Nobuhiko Abe Kentaro Itokawa Madoka Nakai Syun-ichi Urayama Yuto Chiba Michael Amoa-Bosompem Yasuhisa Kunimi Late Male-Killing Viruses in Homona magnanima Identified as Osugoroshi Viruses, Novel Members of Partitiviridae Frontiers in Microbiology male killing Homona magnanima Partitiviridae horizontal transmission insect virus |
| title | Late Male-Killing Viruses in Homona magnanima Identified as Osugoroshi Viruses, Novel Members of Partitiviridae |
| title_full | Late Male-Killing Viruses in Homona magnanima Identified as Osugoroshi Viruses, Novel Members of Partitiviridae |
| title_fullStr | Late Male-Killing Viruses in Homona magnanima Identified as Osugoroshi Viruses, Novel Members of Partitiviridae |
| title_full_unstemmed | Late Male-Killing Viruses in Homona magnanima Identified as Osugoroshi Viruses, Novel Members of Partitiviridae |
| title_short | Late Male-Killing Viruses in Homona magnanima Identified as Osugoroshi Viruses, Novel Members of Partitiviridae |
| title_sort | late male killing viruses in homona magnanima identified as osugoroshi viruses novel members of partitiviridae |
| topic | male killing Homona magnanima Partitiviridae horizontal transmission insect virus |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2020.620623/full |
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