| الملخص: | The variable diapause features of bivoltine silkworm (<i>Bombyx mori</i>) strains regulated by environmental signals in the embryonic stage are closely related to epigenetics. Previously, we showed that the expression of YTHDF3 is significantly different in the pupae of the bivoltine silkworm Qiufeng developed from eggs incubated at a normal temperature (QFHT, diapause egg producer) compared to those from eggs incubated at a low temperature (QFLT, nondiapause egg producer), indicating that the expression of diapause-associated genes is regulated by the m<sup>6</sup>A modification level. However, how YTHDF3 regulates the expression of diapause-related genes remains unclear. In this study, we observed that the knockdown of <i>B. mori</i> YTHDF3 resulted in delayed embryo development, while the overexpression of YTHDF3 resulted in the transformation of nondiapause-destined eggs into a mixture of diapause and nondiapause eggs. Further studies showed that YTHDF3, as a reading protein, can recognize the m<sup>6</sup>A site of <i>Cyp307a1</i> and <i>Cyp18a1</i> genes in the ecdysone synthesis pathway (ESP), and the overexpression of YTHDF3 affects the diapause traits of the silkworm by decreasing the stabilities of mRNAs of <i>Cyp307a1</i> and <i>Cyp18a1</i> and inhibiting their translation. The above results demonstrate that m<sup>6</sup>A modification mediates YTHDF3 to affect the expression levels of its target genes, <i>Cyp307a1</i> and <i>Cyp18a1</i>, in the ESP to regulate diapause in bivoltine <i>B. mori</i>. This is the first report of the m<sup>6</sup>A methylation regulation mechanism in diapause in <i>B. mori</i> and provides new experimental data for clarifying the diapause regulation network.
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