Identification of SALL4 Expressing Islet-1+ Cardiovascular Progenitor Cell Clones
The utilization of cardiac progenitor cells (CPCs) has been shown to induce favorable regenerative effects. While there are various populations of endogenous CPCs in the heart, there is no consensus regarding which population is ideal for cell-based regenerative therapy. Early-stage progenitor cells...
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| Format: | Article |
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MDPI AG
2023-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/2/1780 |
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| author | Andrea Monteon Lorelei Hughes Victor Camberos Mary Kearns-Jonker |
| author_facet | Andrea Monteon Lorelei Hughes Victor Camberos Mary Kearns-Jonker |
| author_sort | Andrea Monteon |
| collection | DOAJ |
| description | The utilization of cardiac progenitor cells (CPCs) has been shown to induce favorable regenerative effects. While there are various populations of endogenous CPCs in the heart, there is no consensus regarding which population is ideal for cell-based regenerative therapy. Early-stage progenitor cells can be differentiated into all cardiovascular lineages, including cardiomyocytes and endothelial cells. Identifying an Islet-1+ (Isl-1+) early-stage progenitor population with enhanced stemness, multipotency and differentiation potential would be beneficial for the development of novel regenerative therapies. Here, we investigated the transcriptome of human neonatal Isl-1+ CPCs. Isl-1+ human neonatal CPCs exhibit enhanced stemness properties and were found to express Spalt-like transcription factor 4 (SALL4). SALL4 plays a role in embryonic development as well as proliferation and expansion of hematopoietic progenitor cells. SALL4, SOX2, EpCAM and TBX5 are co-expressed in the majority of Isl-1+ clones isolated from neonatal patients. The pre-mesendodermal transcript TFAP2C was identified in select Isl-1, SALL4, SOX2, EpCAM and TBX5 expressing clones. The ability to isolate and expand pre-mesendodermal stage cells from human patients is a novel finding that holds potential value for applications in regenerative medicine. |
| first_indexed | 2024-03-09T12:17:14Z |
| format | Article |
| id | doaj.art-df325dd47ee446c2a9210cd683a1909c |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T12:17:14Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-df325dd47ee446c2a9210cd683a1909c2023-11-30T22:45:31ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-01-01242178010.3390/ijms24021780Identification of SALL4 Expressing Islet-1+ Cardiovascular Progenitor Cell ClonesAndrea Monteon0Lorelei Hughes1Victor Camberos2Mary Kearns-Jonker3Department of Pathology and Human Anatomy, Loma Linda University School of Medicine, Loma Linda, CA 92350, USADepartment of Pathology and Human Anatomy, Loma Linda University School of Medicine, Loma Linda, CA 92350, USADepartment of Pathology and Human Anatomy, Loma Linda University School of Medicine, Loma Linda, CA 92350, USADepartment of Pathology and Human Anatomy, Loma Linda University School of Medicine, Loma Linda, CA 92350, USAThe utilization of cardiac progenitor cells (CPCs) has been shown to induce favorable regenerative effects. While there are various populations of endogenous CPCs in the heart, there is no consensus regarding which population is ideal for cell-based regenerative therapy. Early-stage progenitor cells can be differentiated into all cardiovascular lineages, including cardiomyocytes and endothelial cells. Identifying an Islet-1+ (Isl-1+) early-stage progenitor population with enhanced stemness, multipotency and differentiation potential would be beneficial for the development of novel regenerative therapies. Here, we investigated the transcriptome of human neonatal Isl-1+ CPCs. Isl-1+ human neonatal CPCs exhibit enhanced stemness properties and were found to express Spalt-like transcription factor 4 (SALL4). SALL4 plays a role in embryonic development as well as proliferation and expansion of hematopoietic progenitor cells. SALL4, SOX2, EpCAM and TBX5 are co-expressed in the majority of Isl-1+ clones isolated from neonatal patients. The pre-mesendodermal transcript TFAP2C was identified in select Isl-1, SALL4, SOX2, EpCAM and TBX5 expressing clones. The ability to isolate and expand pre-mesendodermal stage cells from human patients is a novel finding that holds potential value for applications in regenerative medicine.https://www.mdpi.com/1422-0067/24/2/1780Islet-1cardiovascular progenitor cellsSALL4 |
| spellingShingle | Andrea Monteon Lorelei Hughes Victor Camberos Mary Kearns-Jonker Identification of SALL4 Expressing Islet-1+ Cardiovascular Progenitor Cell Clones International Journal of Molecular Sciences Islet-1 cardiovascular progenitor cells SALL4 |
| title | Identification of SALL4 Expressing Islet-1+ Cardiovascular Progenitor Cell Clones |
| title_full | Identification of SALL4 Expressing Islet-1+ Cardiovascular Progenitor Cell Clones |
| title_fullStr | Identification of SALL4 Expressing Islet-1+ Cardiovascular Progenitor Cell Clones |
| title_full_unstemmed | Identification of SALL4 Expressing Islet-1+ Cardiovascular Progenitor Cell Clones |
| title_short | Identification of SALL4 Expressing Islet-1+ Cardiovascular Progenitor Cell Clones |
| title_sort | identification of sall4 expressing islet 1 cardiovascular progenitor cell clones |
| topic | Islet-1 cardiovascular progenitor cells SALL4 |
| url | https://www.mdpi.com/1422-0067/24/2/1780 |
| work_keys_str_mv | AT andreamonteon identificationofsall4expressingislet1cardiovascularprogenitorcellclones AT loreleihughes identificationofsall4expressingislet1cardiovascularprogenitorcellclones AT victorcamberos identificationofsall4expressingislet1cardiovascularprogenitorcellclones AT marykearnsjonker identificationofsall4expressingislet1cardiovascularprogenitorcellclones |