Metabolomics analysis of buck semen cryopreserved with trehalose
Trehalose is commonly used as an impermeable cryoprotectant for cryopreservation of cells, but its cryoprotective mechanism has now not but been determined. This study investigated the cryopreservation impact of trehalose on buck semen cryopreservation and finished metabolic profiling of freeze-thaw...
| Hlavní autoři: | , , , , , , , , |
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| Médium: | Článek |
| Jazyk: | English |
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Frontiers Media S.A.
2022-08-01
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| Edice: | Frontiers in Genetics |
| Témata: | |
| On-line přístup: | https://www.frontiersin.org/articles/10.3389/fgene.2022.938622/full |
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| author | Bingbing Xu Bingbing Xu Bingbing Xu Zhiying Wang Zhiying Wang Zhiying Wang Ruijun Wang Ruijun Wang Ruijun Wang Guoxin Song Guoxin Song Guoxin Song Yanjun Zhang Yanjun Zhang Yanjun Zhang Rui Su Rui Su Rui Su Yongbin Liu Jinquan Li Jinquan Li Jinquan Li Jiaxin Zhang Jiaxin Zhang Jiaxin Zhang |
| author_facet | Bingbing Xu Bingbing Xu Bingbing Xu Zhiying Wang Zhiying Wang Zhiying Wang Ruijun Wang Ruijun Wang Ruijun Wang Guoxin Song Guoxin Song Guoxin Song Yanjun Zhang Yanjun Zhang Yanjun Zhang Rui Su Rui Su Rui Su Yongbin Liu Jinquan Li Jinquan Li Jinquan Li Jiaxin Zhang Jiaxin Zhang Jiaxin Zhang |
| author_sort | Bingbing Xu |
| collection | DOAJ |
| description | Trehalose is commonly used as an impermeable cryoprotectant for cryopreservation of cells, but its cryoprotective mechanism has now not but been determined. This study investigated the cryopreservation impact of trehalose on buck semen cryopreservation and finished metabolic profiling of freeze-thawed media by way of the GC–MS-based metabolomics for the first time. Metabolic pattern recognition and metabolite identification by means of principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and metabolic pathway topology analysis revealed the results of trehalose on buck sperm metabolism at some point of cryopreservation. The results confirmed that trehalose drastically progressed sperm motility parameters and structural integrity after thawing. PCA and PLS-DA analysis discovered that the metabolic patterns of the freezing-thawing media of buck semen cryopreserved with trehalose (T group) or without trehalose (G group, Control) were certainly separated. Using screening conditions of VIP >1.5 and p vaule <0.05, a total of 48 differential metabolites have been recognized, whithin l-isoleucine, L-leucine, L-threonine, and dihydroxyacetone were notably enriched in valine, leucine and isoleucine biosynthesis, glycerolipid metabolism, and aminoacyl-tRNA biosynthesis pathways. In brief, trehalose can efficiently improve membrane structural integrity and motion parameters in buck sperm after thawing, and it exerts a cryoprotective impact with the aid of changing sperm amino acid synthesis and the glycerol metabolism pathway. |
| first_indexed | 2024-12-10T17:29:11Z |
| format | Article |
| id | doaj.art-df32ee20b51d4b39ba50dfa034561b51 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-10T17:29:11Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-df32ee20b51d4b39ba50dfa034561b512022-12-22T01:39:44ZengFrontiers Media S.A.Frontiers in Genetics1664-80212022-08-011310.3389/fgene.2022.938622938622Metabolomics analysis of buck semen cryopreserved with trehaloseBingbing Xu0Bingbing Xu1Bingbing Xu2Zhiying Wang3Zhiying Wang4Zhiying Wang5Ruijun Wang6Ruijun Wang7Ruijun Wang8Guoxin Song9Guoxin Song10Guoxin Song11Yanjun Zhang12Yanjun Zhang13Yanjun Zhang14Rui Su15Rui Su16Rui Su17Yongbin Liu18Jinquan Li19Jinquan Li20Jinquan Li21Jiaxin Zhang22Jiaxin Zhang23Jiaxin Zhang24College of Animal Science, Inner Mongolia Agricultural University, Hohhot, ChinaInner Mongolia Key Laboratory of Animal Genetics, Breeding, and Reproduction, Hohhot, ChinaKey Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Hohhot, ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot, ChinaInner Mongolia Key Laboratory of Animal Genetics, Breeding, and Reproduction, Hohhot, ChinaKey Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Hohhot, ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot, ChinaInner Mongolia Key Laboratory of Animal Genetics, Breeding, and Reproduction, Hohhot, ChinaKey Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Hohhot, ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot, ChinaInner Mongolia Key Laboratory of Animal Genetics, Breeding, and Reproduction, Hohhot, ChinaKey Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Hohhot, ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot, ChinaInner Mongolia Key Laboratory of Animal Genetics, Breeding, and Reproduction, Hohhot, ChinaKey Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Hohhot, ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot, ChinaInner Mongolia Key Laboratory of Animal Genetics, Breeding, and Reproduction, Hohhot, ChinaKey Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Hohhot, ChinaResearch Center for Animal Genetic Resources of Mongolian Plateau, College of Life Sciences, Inner Mongolia University, Hohhot, ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot, ChinaInner Mongolia Key Laboratory of Animal Genetics, Breeding, and Reproduction, Hohhot, ChinaKey Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Hohhot, ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot, ChinaInner Mongolia Key Laboratory of Animal Genetics, Breeding, and Reproduction, Hohhot, ChinaKey Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Hohhot, ChinaTrehalose is commonly used as an impermeable cryoprotectant for cryopreservation of cells, but its cryoprotective mechanism has now not but been determined. This study investigated the cryopreservation impact of trehalose on buck semen cryopreservation and finished metabolic profiling of freeze-thawed media by way of the GC–MS-based metabolomics for the first time. Metabolic pattern recognition and metabolite identification by means of principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and metabolic pathway topology analysis revealed the results of trehalose on buck sperm metabolism at some point of cryopreservation. The results confirmed that trehalose drastically progressed sperm motility parameters and structural integrity after thawing. PCA and PLS-DA analysis discovered that the metabolic patterns of the freezing-thawing media of buck semen cryopreserved with trehalose (T group) or without trehalose (G group, Control) were certainly separated. Using screening conditions of VIP >1.5 and p vaule <0.05, a total of 48 differential metabolites have been recognized, whithin l-isoleucine, L-leucine, L-threonine, and dihydroxyacetone were notably enriched in valine, leucine and isoleucine biosynthesis, glycerolipid metabolism, and aminoacyl-tRNA biosynthesis pathways. In brief, trehalose can efficiently improve membrane structural integrity and motion parameters in buck sperm after thawing, and it exerts a cryoprotective impact with the aid of changing sperm amino acid synthesis and the glycerol metabolism pathway.https://www.frontiersin.org/articles/10.3389/fgene.2022.938622/fullbuckspermatozoacryopreservationtrehalosemetabolomics |
| spellingShingle | Bingbing Xu Bingbing Xu Bingbing Xu Zhiying Wang Zhiying Wang Zhiying Wang Ruijun Wang Ruijun Wang Ruijun Wang Guoxin Song Guoxin Song Guoxin Song Yanjun Zhang Yanjun Zhang Yanjun Zhang Rui Su Rui Su Rui Su Yongbin Liu Jinquan Li Jinquan Li Jinquan Li Jiaxin Zhang Jiaxin Zhang Jiaxin Zhang Metabolomics analysis of buck semen cryopreserved with trehalose Frontiers in Genetics buck spermatozoa cryopreservation trehalose metabolomics |
| title | Metabolomics analysis of buck semen cryopreserved with trehalose |
| title_full | Metabolomics analysis of buck semen cryopreserved with trehalose |
| title_fullStr | Metabolomics analysis of buck semen cryopreserved with trehalose |
| title_full_unstemmed | Metabolomics analysis of buck semen cryopreserved with trehalose |
| title_short | Metabolomics analysis of buck semen cryopreserved with trehalose |
| title_sort | metabolomics analysis of buck semen cryopreserved with trehalose |
| topic | buck spermatozoa cryopreservation trehalose metabolomics |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2022.938622/full |
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