Association of p53 Codon 72 Polymorphism with Risk of Hypopharyngeal Squamous Cell Carcinoma in Taiwan
p53 polymorphism at codon 72 is a known risk marker for various malignancies, but it has not been studied in hypopharyngeal cancer. This study investigated the genotype distribution of p53 codon 72 polymorphism in hypopharyngeal cancer patients and non-cancer controls matched for age, gender, alcoho...
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| Format: | Article |
| Language: | English |
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Elsevier
2006-01-01
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| Series: | Journal of the Formosan Medical Association |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0929664609603302 |
| _version_ | 1818943429038571520 |
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| author | Chih-Wen Twu Rong-San Jiang Chih-Hung Shu Jin-Ching Lin |
| author_facet | Chih-Wen Twu Rong-San Jiang Chih-Hung Shu Jin-Ching Lin |
| author_sort | Chih-Wen Twu |
| collection | DOAJ |
| description | p53 polymorphism at codon 72 is a known risk marker for various malignancies, but it has not been studied in hypopharyngeal cancer. This study investigated the genotype distribution of p53 codon 72 polymorphism in hypopharyngeal cancer patients and non-cancer controls matched for age, gender, alcohol consumption and smoking habit.
Methods: Genomic DNA was extracted from peripheral blood cells of 53 patients with hypopharyngeal cancer and 53 non-cancer controls. Codon 72 polymorphism of p53 was identified by polymerase chain reaction-restriction fragment length polymorphism.
Results: Patients with hypopharyngeal cancer had higher frequencies of Pro/Pro (26.4% vs. 13.2%) and Pro/Arg (51.0% vs. 45.3%) but lower frequencies of Arg/Arg (22.6% vs. 45.1%) compared to controls. Compared to Arg/Arg genotypes, Pro/Pro genotypes had a relative risk of hypopharyngeal cancer of 3.667 (95% confidence interval, 1.16-11.56; p = 0.03). As a group, patients with Pro/Pro or Arg/Pro who were carriers of the Pro allele had a higher relative risk of hypopharyngeal cancer compared to Arg homozygous carriers (odds ratio, 2.415; 95% confidence interval, 1.04-5.64; p = 0.04).
Conclusion: This study demonstrated that p53 codon 72 Pro homozygosity is associated with a higher risk of developing hypopharyngeal cancer. |
| first_indexed | 2024-12-20T07:27:11Z |
| format | Article |
| id | doaj.art-df34adeab1d74013bbfa9b99ffa4189f |
| institution | Directory Open Access Journal |
| issn | 0929-6646 |
| language | English |
| last_indexed | 2024-12-20T07:27:11Z |
| publishDate | 2006-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of the Formosan Medical Association |
| spelling | doaj.art-df34adeab1d74013bbfa9b99ffa4189f2022-12-21T19:48:30ZengElsevierJournal of the Formosan Medical Association0929-66462006-01-0110529910410.1016/S0929-6646(09)60330-2Association of p53 Codon 72 Polymorphism with Risk of Hypopharyngeal Squamous Cell Carcinoma in TaiwanChih-Wen Twu0Rong-San Jiang1Chih-Hung Shu2Jin-Ching Lin3Institute of Clinical Medicine, National Yang-Ming University, TaipeiDepartment of Otolaryngology, Taichung Veterans General Hospital, Taichung, Taiwan, R.O.C.Department of Otolaryngology, Taichung Veterans General Hospital, Taichung, Taiwan, R.O.C.Institute of Clinical Medicine, National Yang-Ming University, Taipeip53 polymorphism at codon 72 is a known risk marker for various malignancies, but it has not been studied in hypopharyngeal cancer. This study investigated the genotype distribution of p53 codon 72 polymorphism in hypopharyngeal cancer patients and non-cancer controls matched for age, gender, alcohol consumption and smoking habit. Methods: Genomic DNA was extracted from peripheral blood cells of 53 patients with hypopharyngeal cancer and 53 non-cancer controls. Codon 72 polymorphism of p53 was identified by polymerase chain reaction-restriction fragment length polymorphism. Results: Patients with hypopharyngeal cancer had higher frequencies of Pro/Pro (26.4% vs. 13.2%) and Pro/Arg (51.0% vs. 45.3%) but lower frequencies of Arg/Arg (22.6% vs. 45.1%) compared to controls. Compared to Arg/Arg genotypes, Pro/Pro genotypes had a relative risk of hypopharyngeal cancer of 3.667 (95% confidence interval, 1.16-11.56; p = 0.03). As a group, patients with Pro/Pro or Arg/Pro who were carriers of the Pro allele had a higher relative risk of hypopharyngeal cancer compared to Arg homozygous carriers (odds ratio, 2.415; 95% confidence interval, 1.04-5.64; p = 0.04). Conclusion: This study demonstrated that p53 codon 72 Pro homozygosity is associated with a higher risk of developing hypopharyngeal cancer.http://www.sciencedirect.com/science/article/pii/S0929664609603302hypopharyngeal cancerp53polymorphism |
| spellingShingle | Chih-Wen Twu Rong-San Jiang Chih-Hung Shu Jin-Ching Lin Association of p53 Codon 72 Polymorphism with Risk of Hypopharyngeal Squamous Cell Carcinoma in Taiwan Journal of the Formosan Medical Association hypopharyngeal cancer p53 polymorphism |
| title | Association of p53 Codon 72 Polymorphism with Risk of Hypopharyngeal Squamous Cell Carcinoma in Taiwan |
| title_full | Association of p53 Codon 72 Polymorphism with Risk of Hypopharyngeal Squamous Cell Carcinoma in Taiwan |
| title_fullStr | Association of p53 Codon 72 Polymorphism with Risk of Hypopharyngeal Squamous Cell Carcinoma in Taiwan |
| title_full_unstemmed | Association of p53 Codon 72 Polymorphism with Risk of Hypopharyngeal Squamous Cell Carcinoma in Taiwan |
| title_short | Association of p53 Codon 72 Polymorphism with Risk of Hypopharyngeal Squamous Cell Carcinoma in Taiwan |
| title_sort | association of p53 codon 72 polymorphism with risk of hypopharyngeal squamous cell carcinoma in taiwan |
| topic | hypopharyngeal cancer p53 polymorphism |
| url | http://www.sciencedirect.com/science/article/pii/S0929664609603302 |
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