Magnetic covalent organic framework nanospheres-based miRNA biosensor for sensitive glioma detection
Sensitive detection and accurate diagnosis/prognosis of glioma remain urgent challenges. Herein, dispersed magnetic covalent organic framework nanospheres (MCOF) with uniformed Fe3O4 nano-assembly as cores and high-crystalline COF as shells were prepared by monomer-mediated in-situ interface growth...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2022-08-01
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| Series: | Bioactive Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X21005764 |
| _version_ | 1797204496300900352 |
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| author | Dong Liang Xiaoyi Zhang Yi Wang Taotao Huo Min Qian Yibo Xie Wenshuai Li Yunqiu Yu Wei Shi Qianqian Liu Junle Zhu Chun Luo Zhijuan Cao Rongqin Huang |
| author_facet | Dong Liang Xiaoyi Zhang Yi Wang Taotao Huo Min Qian Yibo Xie Wenshuai Li Yunqiu Yu Wei Shi Qianqian Liu Junle Zhu Chun Luo Zhijuan Cao Rongqin Huang |
| author_sort | Dong Liang |
| collection | DOAJ |
| description | Sensitive detection and accurate diagnosis/prognosis of glioma remain urgent challenges. Herein, dispersed magnetic covalent organic framework nanospheres (MCOF) with uniformed Fe3O4 nano-assembly as cores and high-crystalline COF as shells were prepared by monomer-mediated in-situ interface growth strategy. Based on the unique interaction between MCOF and hairpin DNA, a fluorescent signal amplified miRNA biosensor was constructed. It could realize the sensitive detection of miRNA-182 in different matrixes, where the detection limit, linearity range and determination coefficient (R2) in real blood samples reached 20 fM, 0.1 pM–10 pM and 0.991, respectively. Also, it possessed good stability and precision as observed from the low intra-day/inter-day RSD and high extraction recovery. As a result, it could quantify miRNA-182 in serum of glioma patients, the concentration of which was significantly higher than that of healthy people and obviously decreased after surgery. Finally, a proof-of-concept capillary chip system using this biosensor was proposed to realize the visualized detection of miRNA-182 in microsample. These findings suggest a robust way for sensitive detection and accurate diagnosis/prognosis of glioma. |
| first_indexed | 2024-04-13T01:11:33Z |
| format | Article |
| id | doaj.art-df37bb0217fa4da6a30dd4c014fd3380 |
| institution | Directory Open Access Journal |
| issn | 2452-199X |
| language | English |
| last_indexed | 2024-04-24T08:36:09Z |
| publishDate | 2022-08-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Bioactive Materials |
| spelling | doaj.art-df37bb0217fa4da6a30dd4c014fd33802024-04-16T17:16:32ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2022-08-0114145151Magnetic covalent organic framework nanospheres-based miRNA biosensor for sensitive glioma detectionDong Liang0Xiaoyi Zhang1Yi Wang2Taotao Huo3Min Qian4Yibo Xie5Wenshuai Li6Yunqiu Yu7Wei Shi8Qianqian Liu9Junle Zhu10Chun Luo11Zhijuan Cao12Rongqin Huang13School of Pharmacy, Key Laboratory of Smart Drug Delivery, Ministry of Education, Fudan University, Shanghai, 201203, ChinaSchool of Pharmacy, Key Laboratory of Smart Drug Delivery, Ministry of Education, Fudan University, Shanghai, 201203, ChinaCenter for Advanced Low-dimension Materials, State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, 201600, China; Corresponding author.School of Pharmacy, Key Laboratory of Smart Drug Delivery, Ministry of Education, Fudan University, Shanghai, 201203, ChinaSchool of Pharmacy, Key Laboratory of Smart Drug Delivery, Ministry of Education, Fudan University, Shanghai, 201203, ChinaSchool of Pharmacy, Key Laboratory of Smart Drug Delivery, Ministry of Education, Fudan University, Shanghai, 201203, ChinaSchool of Pharmacy, Key Laboratory of Smart Drug Delivery, Ministry of Education, Fudan University, Shanghai, 201203, ChinaSchool of Pharmacy, Key Laboratory of Smart Drug Delivery, Ministry of Education, Fudan University, Shanghai, 201203, China; Corresponding author.Department of Neurosurgery, Affiliated Hospital of Nantong University, Nantong, 226001, ChinaDepartment of Neurosurgery, Affiliated Hospital of Nantong University, Nantong, 226001, ChinaDepartment of Neurosurgery, Tongji Hospital, Tongji University School of Medicine, Shanghai, 200065, ChinaDepartment of Neurosurgery, Tongji Hospital, Tongji University School of Medicine, Shanghai, 200065, ChinaSchool of Pharmacy, Key Laboratory of Smart Drug Delivery, Ministry of Education, Fudan University, Shanghai, 201203, ChinaSchool of Pharmacy, Key Laboratory of Smart Drug Delivery, Ministry of Education, Fudan University, Shanghai, 201203, China; Corresponding author.Sensitive detection and accurate diagnosis/prognosis of glioma remain urgent challenges. Herein, dispersed magnetic covalent organic framework nanospheres (MCOF) with uniformed Fe3O4 nano-assembly as cores and high-crystalline COF as shells were prepared by monomer-mediated in-situ interface growth strategy. Based on the unique interaction between MCOF and hairpin DNA, a fluorescent signal amplified miRNA biosensor was constructed. It could realize the sensitive detection of miRNA-182 in different matrixes, where the detection limit, linearity range and determination coefficient (R2) in real blood samples reached 20 fM, 0.1 pM–10 pM and 0.991, respectively. Also, it possessed good stability and precision as observed from the low intra-day/inter-day RSD and high extraction recovery. As a result, it could quantify miRNA-182 in serum of glioma patients, the concentration of which was significantly higher than that of healthy people and obviously decreased after surgery. Finally, a proof-of-concept capillary chip system using this biosensor was proposed to realize the visualized detection of miRNA-182 in microsample. These findings suggest a robust way for sensitive detection and accurate diagnosis/prognosis of glioma.http://www.sciencedirect.com/science/article/pii/S2452199X21005764Blood samplesFluorescence signal amplification biosensorGlioma diagnosisMagnetic COF nanospheresmiRNA detection |
| spellingShingle | Dong Liang Xiaoyi Zhang Yi Wang Taotao Huo Min Qian Yibo Xie Wenshuai Li Yunqiu Yu Wei Shi Qianqian Liu Junle Zhu Chun Luo Zhijuan Cao Rongqin Huang Magnetic covalent organic framework nanospheres-based miRNA biosensor for sensitive glioma detection Bioactive Materials Blood samples Fluorescence signal amplification biosensor Glioma diagnosis Magnetic COF nanospheres miRNA detection |
| title | Magnetic covalent organic framework nanospheres-based miRNA biosensor for sensitive glioma detection |
| title_full | Magnetic covalent organic framework nanospheres-based miRNA biosensor for sensitive glioma detection |
| title_fullStr | Magnetic covalent organic framework nanospheres-based miRNA biosensor for sensitive glioma detection |
| title_full_unstemmed | Magnetic covalent organic framework nanospheres-based miRNA biosensor for sensitive glioma detection |
| title_short | Magnetic covalent organic framework nanospheres-based miRNA biosensor for sensitive glioma detection |
| title_sort | magnetic covalent organic framework nanospheres based mirna biosensor for sensitive glioma detection |
| topic | Blood samples Fluorescence signal amplification biosensor Glioma diagnosis Magnetic COF nanospheres miRNA detection |
| url | http://www.sciencedirect.com/science/article/pii/S2452199X21005764 |
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