High-Resolution Magnetic Resonance Imaging of the Regenerating Adult Zebrafish Heart
Abstract The adult zebrafish is a well-established model for studying heart regeneration, but due to its tissue opaqueness, repair has been primarily assessed using destructive histology, precluding repeated investigations of the same animal. We present a high-resolution, non-invasive in vivo magnet...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2017-06-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-03050-y |
| _version_ | 1818750802783633408 |
|---|---|
| author | Jana Koth Mahon L. Maguire Darryl McClymont Leonie Diffley Victoria L. Thornton John Beech Roger K. Patient Paul R. Riley Jürgen E. Schneider |
| author_facet | Jana Koth Mahon L. Maguire Darryl McClymont Leonie Diffley Victoria L. Thornton John Beech Roger K. Patient Paul R. Riley Jürgen E. Schneider |
| author_sort | Jana Koth |
| collection | DOAJ |
| description | Abstract The adult zebrafish is a well-established model for studying heart regeneration, but due to its tissue opaqueness, repair has been primarily assessed using destructive histology, precluding repeated investigations of the same animal. We present a high-resolution, non-invasive in vivo magnetic resonance imaging (MRI) method incorporating a miniature respiratory and anaesthetic perfusion set-up for live adult zebrafish, allowing for visualization of scar formation and heart regeneration in the same animal over time at an isotropic 31 µm voxel resolution. To test the method, we compared well and poorly healing cardiac ventricles using a transgenic fish model that exhibits heat-shock (HS) inducible impaired heart regeneration. HS-treated groups revealed persistent scar tissue for 10 weeks, while control groups were healed after 4 weeks. Application of the advanced MRI technique allowed clear discrimination of levels of repair following cryo- and resection injury for several months. It further provides a novel tool for in vivo time-lapse imaging of adult fish for non-cardiac studies, as the method can be readily applied to image wound healing in other injured or diseased tissues, or to monitor tissue changes over time, thus expanding the range of questions that can be addressed in adult zebrafish and other small aquatic species. |
| first_indexed | 2024-12-18T04:25:28Z |
| format | Article |
| id | doaj.art-df3a001c45664798981cda1674ac34b4 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-18T04:25:28Z |
| publishDate | 2017-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-df3a001c45664798981cda1674ac34b42022-12-21T21:21:08ZengNature PortfolioScientific Reports2045-23222017-06-017111210.1038/s41598-017-03050-yHigh-Resolution Magnetic Resonance Imaging of the Regenerating Adult Zebrafish HeartJana Koth0Mahon L. Maguire1Darryl McClymont2Leonie Diffley3Victoria L. Thornton4John Beech5Roger K. Patient6Paul R. Riley7Jürgen E. Schneider8Weatherall Institute of Molecular Medicine, JR Hospital, Oxford UniversityBHF Experimental MR Unit, Wellcome Trust Centre for Human Genetics, Oxford UniversityBHF Experimental MR Unit, Wellcome Trust Centre for Human Genetics, Oxford UniversityBHF Experimental MR Unit, Wellcome Trust Centre for Human Genetics, Oxford UniversityBHF Experimental MR Unit, Wellcome Trust Centre for Human Genetics, Oxford UniversityDepartment of Oncology, Oxford UniversityWeatherall Institute of Molecular Medicine, JR Hospital, Oxford UniversityDepartment of Physiology, Anatomy and Genetics, Oxford UniversityBHF Experimental MR Unit, Wellcome Trust Centre for Human Genetics, Oxford UniversityAbstract The adult zebrafish is a well-established model for studying heart regeneration, but due to its tissue opaqueness, repair has been primarily assessed using destructive histology, precluding repeated investigations of the same animal. We present a high-resolution, non-invasive in vivo magnetic resonance imaging (MRI) method incorporating a miniature respiratory and anaesthetic perfusion set-up for live adult zebrafish, allowing for visualization of scar formation and heart regeneration in the same animal over time at an isotropic 31 µm voxel resolution. To test the method, we compared well and poorly healing cardiac ventricles using a transgenic fish model that exhibits heat-shock (HS) inducible impaired heart regeneration. HS-treated groups revealed persistent scar tissue for 10 weeks, while control groups were healed after 4 weeks. Application of the advanced MRI technique allowed clear discrimination of levels of repair following cryo- and resection injury for several months. It further provides a novel tool for in vivo time-lapse imaging of adult fish for non-cardiac studies, as the method can be readily applied to image wound healing in other injured or diseased tissues, or to monitor tissue changes over time, thus expanding the range of questions that can be addressed in adult zebrafish and other small aquatic species.https://doi.org/10.1038/s41598-017-03050-y |
| spellingShingle | Jana Koth Mahon L. Maguire Darryl McClymont Leonie Diffley Victoria L. Thornton John Beech Roger K. Patient Paul R. Riley Jürgen E. Schneider High-Resolution Magnetic Resonance Imaging of the Regenerating Adult Zebrafish Heart Scientific Reports |
| title | High-Resolution Magnetic Resonance Imaging of the Regenerating Adult Zebrafish Heart |
| title_full | High-Resolution Magnetic Resonance Imaging of the Regenerating Adult Zebrafish Heart |
| title_fullStr | High-Resolution Magnetic Resonance Imaging of the Regenerating Adult Zebrafish Heart |
| title_full_unstemmed | High-Resolution Magnetic Resonance Imaging of the Regenerating Adult Zebrafish Heart |
| title_short | High-Resolution Magnetic Resonance Imaging of the Regenerating Adult Zebrafish Heart |
| title_sort | high resolution magnetic resonance imaging of the regenerating adult zebrafish heart |
| url | https://doi.org/10.1038/s41598-017-03050-y |
| work_keys_str_mv | AT janakoth highresolutionmagneticresonanceimagingoftheregeneratingadultzebrafishheart AT mahonlmaguire highresolutionmagneticresonanceimagingoftheregeneratingadultzebrafishheart AT darrylmcclymont highresolutionmagneticresonanceimagingoftheregeneratingadultzebrafishheart AT leoniediffley highresolutionmagneticresonanceimagingoftheregeneratingadultzebrafishheart AT victorialthornton highresolutionmagneticresonanceimagingoftheregeneratingadultzebrafishheart AT johnbeech highresolutionmagneticresonanceimagingoftheregeneratingadultzebrafishheart AT rogerkpatient highresolutionmagneticresonanceimagingoftheregeneratingadultzebrafishheart AT paulrriley highresolutionmagneticresonanceimagingoftheregeneratingadultzebrafishheart AT jurgeneschneider highresolutionmagneticresonanceimagingoftheregeneratingadultzebrafishheart |