Effect of the First Feeding on Enterocytes of Newborn Rats
The transcytosis of lipids through enterocytes occurs through the delivery of lipid micelles to the microvilli of enterocytes, consumption of lipid derivates by the apical plasma membrane (PM) and then their delivery to the membrane of the smooth ER attached to the basolateral PM. The SER forms imma...
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MDPI AG
2022-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/22/14179 |
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| author | Maria A. Nikonova Irina S. Sesorova Ivan D. Dimov Natalia R. Karelina Alexander A. Mironov |
| author_facet | Maria A. Nikonova Irina S. Sesorova Ivan D. Dimov Natalia R. Karelina Alexander A. Mironov |
| author_sort | Maria A. Nikonova |
| collection | DOAJ |
| description | The transcytosis of lipids through enterocytes occurs through the delivery of lipid micelles to the microvilli of enterocytes, consumption of lipid derivates by the apical plasma membrane (PM) and then their delivery to the membrane of the smooth ER attached to the basolateral PM. The SER forms immature chylomicrons (iChMs) in the ER lumen. iChMs are delivered at the Golgi complex (GC) where they are subjected to additional glycosylation resulting in maturation of iChMs. ChMs are secreted into the intercellular space and delivered into the lumen of lymphatic capillaries (LCs). The overloading of enterocytes with lipids induces the formation of lipid droplets inside the lipid bilayer of the ER membranes and transcytosis becomes slower. Here, we examined components of the enterocyte-to-lymphatic barriers in newly born rats before the first feeding and after it. In contrast to adult animals, enterocytes of newborns rats exhibited apical endocytosis and a well-developed subapical endosomal tubular network. These enterocytes uptake membranes from amniotic fluid. Then these membranes are transported across the polarized GC and secreted into the intercellular space. The enterocytes did not contain COPII-coated buds on the granular ER. The endothelium of blood capillaries situated near the enterocytes contained only a few fenestrae. The LCs were similar to those in adult animals. The first feeding induced specific alterations of enterocytes, which were similar to those observed after the lipid overloading of enterocytes in adult rats. Enlarged chylomicrons were stopped at the level of the LAMP2 and Neu1 positive post-Golgi structures, secreted, fused, delivered to the interstitial space, captured by the LCs and transported to the lymph node, inducing the movement of macrophages from lymphatic follicles into its sinuses. The macrophages captured the ChMs, preventing their delivery into the blood. |
| first_indexed | 2024-03-09T18:17:08Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T18:17:08Z |
| publishDate | 2022-11-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-df3c134b9c7848ad9f72bcde8feb0ddb2023-11-24T08:39:55ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-11-0123221417910.3390/ijms232214179Effect of the First Feeding on Enterocytes of Newborn RatsMaria A. Nikonova0Irina S. Sesorova1Ivan D. Dimov2Natalia R. Karelina3Alexander A. Mironov4Department of Anatomy, Ivanovo State Medical Academy, 153012 Ivanovo, RussiaDepartment of Anatomy, Ivanovo State Medical Academy, 153012 Ivanovo, RussiaDepartment of Anatomy, Saint Petersburg State Pediatric Medical University, 194100 Saint Petersburg, RussiaDepartment of Anatomy, Saint Petersburg State Pediatric Medical University, 194100 Saint Petersburg, RussiaThe AIRC Institute of Molecular Oncology, 20139 Milan, ItalyThe transcytosis of lipids through enterocytes occurs through the delivery of lipid micelles to the microvilli of enterocytes, consumption of lipid derivates by the apical plasma membrane (PM) and then their delivery to the membrane of the smooth ER attached to the basolateral PM. The SER forms immature chylomicrons (iChMs) in the ER lumen. iChMs are delivered at the Golgi complex (GC) where they are subjected to additional glycosylation resulting in maturation of iChMs. ChMs are secreted into the intercellular space and delivered into the lumen of lymphatic capillaries (LCs). The overloading of enterocytes with lipids induces the formation of lipid droplets inside the lipid bilayer of the ER membranes and transcytosis becomes slower. Here, we examined components of the enterocyte-to-lymphatic barriers in newly born rats before the first feeding and after it. In contrast to adult animals, enterocytes of newborns rats exhibited apical endocytosis and a well-developed subapical endosomal tubular network. These enterocytes uptake membranes from amniotic fluid. Then these membranes are transported across the polarized GC and secreted into the intercellular space. The enterocytes did not contain COPII-coated buds on the granular ER. The endothelium of blood capillaries situated near the enterocytes contained only a few fenestrae. The LCs were similar to those in adult animals. The first feeding induced specific alterations of enterocytes, which were similar to those observed after the lipid overloading of enterocytes in adult rats. Enlarged chylomicrons were stopped at the level of the LAMP2 and Neu1 positive post-Golgi structures, secreted, fused, delivered to the interstitial space, captured by the LCs and transported to the lymph node, inducing the movement of macrophages from lymphatic follicles into its sinuses. The macrophages captured the ChMs, preventing their delivery into the blood.https://www.mdpi.com/1422-0067/23/22/14179Golgi complexenterocytesnewbornlipid overloadinglymphatic capillariestranscytosis |
| spellingShingle | Maria A. Nikonova Irina S. Sesorova Ivan D. Dimov Natalia R. Karelina Alexander A. Mironov Effect of the First Feeding on Enterocytes of Newborn Rats International Journal of Molecular Sciences Golgi complex enterocytes newborn lipid overloading lymphatic capillaries transcytosis |
| title | Effect of the First Feeding on Enterocytes of Newborn Rats |
| title_full | Effect of the First Feeding on Enterocytes of Newborn Rats |
| title_fullStr | Effect of the First Feeding on Enterocytes of Newborn Rats |
| title_full_unstemmed | Effect of the First Feeding on Enterocytes of Newborn Rats |
| title_short | Effect of the First Feeding on Enterocytes of Newborn Rats |
| title_sort | effect of the first feeding on enterocytes of newborn rats |
| topic | Golgi complex enterocytes newborn lipid overloading lymphatic capillaries transcytosis |
| url | https://www.mdpi.com/1422-0067/23/22/14179 |
| work_keys_str_mv | AT mariaanikonova effectofthefirstfeedingonenterocytesofnewbornrats AT irinassesorova effectofthefirstfeedingonenterocytesofnewbornrats AT ivanddimov effectofthefirstfeedingonenterocytesofnewbornrats AT nataliarkarelina effectofthefirstfeedingonenterocytesofnewbornrats AT alexanderamironov effectofthefirstfeedingonenterocytesofnewbornrats |