Green Tea Catechins, (−)‐Catechin Gallate, and (−)‐Gallocatechin Gallate are Potent Inhibitors of ABA‐Induced Stomatal Closure
Abstract Stomatal movement is indispensable for plant growth and survival in response to environmental stimuli. Cytosolic Ca2+ elevation plays a crucial role in ABA‐induced stomatal closure during drought stress; however, to what extent the Ca2+ movement across the plasma membrane from the apoplast...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-07-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202201403 |
| _version_ | 1811221845173600256 |
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| author | Kanane Sato Shunya Saito Kohsuke Endo Masaru Kono Taishin Kakei Haruka Taketa Megumi Kato Shin Hamamoto Matteo Grenzi Alex Costa Shintaro Munemasa Yoshiyuki Murata Yasuhiro Ishimaru Nobuyuki Uozumi |
| author_facet | Kanane Sato Shunya Saito Kohsuke Endo Masaru Kono Taishin Kakei Haruka Taketa Megumi Kato Shin Hamamoto Matteo Grenzi Alex Costa Shintaro Munemasa Yoshiyuki Murata Yasuhiro Ishimaru Nobuyuki Uozumi |
| author_sort | Kanane Sato |
| collection | DOAJ |
| description | Abstract Stomatal movement is indispensable for plant growth and survival in response to environmental stimuli. Cytosolic Ca2+ elevation plays a crucial role in ABA‐induced stomatal closure during drought stress; however, to what extent the Ca2+ movement across the plasma membrane from the apoplast to the cytosol contributes to this process still needs clarification. Here the authors identify (−)‐catechin gallate (CG) and (−)‐gallocatechin gallate (GCG), components of green tea, as inhibitors of voltage‐dependent K+ channels which regulate K+ fluxes in Arabidopsis thaliana guard cells. In Arabidopsis guard cells CG/GCG prevent ABA‐induced: i) membrane depolarization; ii) activation of Ca2+ permeable cation (ICa) channels; and iii) cytosolic Ca2+ transients. In whole Arabidopsis plants co‐treatment with CG/GCG and ABA suppressed ABA‐induced stomatal closure and surface temperature increase. Similar to ABA, CG/GCG inhibited stomatal closure is elicited by the elicitor peptide, flg22 but has no impact on dark‐induced stomatal closure or light‐ and fusicoccin‐induced stomatal opening, suggesting that the inhibitory effect of CG/GCG is associated with Ca2+‐related signaling pathways. This study further supports the crucial role of ICa channels of the plasma membrane in ABA‐induced stomatal closure. Moreover, CG and GCG represent a new tool for the study of abiotic or biotic stress‐induced signal transduction pathways. |
| first_indexed | 2024-04-12T08:07:12Z |
| format | Article |
| id | doaj.art-df40ea998954479c90acd2049c755b25 |
| institution | Directory Open Access Journal |
| issn | 2198-3844 |
| language | English |
| last_indexed | 2024-04-12T08:07:12Z |
| publishDate | 2022-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj.art-df40ea998954479c90acd2049c755b252022-12-22T03:41:07ZengWileyAdvanced Science2198-38442022-07-01921n/an/a10.1002/advs.202201403Green Tea Catechins, (−)‐Catechin Gallate, and (−)‐Gallocatechin Gallate are Potent Inhibitors of ABA‐Induced Stomatal ClosureKanane Sato0Shunya Saito1Kohsuke Endo2Masaru Kono3Taishin Kakei4Haruka Taketa5Megumi Kato6Shin Hamamoto7Matteo Grenzi8Alex Costa9Shintaro Munemasa10Yoshiyuki Murata11Yasuhiro Ishimaru12Nobuyuki Uozumi13Department of Biomolecular Engineering Graduate School of Engineering Tohoku University Aobayama 6‐6‐07 Sendai 980‐8579 JapanDepartment of Biomolecular Engineering Graduate School of Engineering Tohoku University Aobayama 6‐6‐07 Sendai 980‐8579 JapanDepartment of Biomolecular Engineering Graduate School of Engineering Tohoku University Aobayama 6‐6‐07 Sendai 980‐8579 JapanDepartment of Biology Graduate School of Science University of Tokyo Bunkyo‐ku 113‐0033 JapanDepartment of Biomolecular Engineering Graduate School of Engineering Tohoku University Aobayama 6‐6‐07 Sendai 980‐8579 JapanDepartment of Biomolecular Engineering Graduate School of Engineering Tohoku University Aobayama 6‐6‐07 Sendai 980‐8579 JapanDepartment of Biomolecular Engineering Graduate School of Engineering Tohoku University Aobayama 6‐6‐07 Sendai 980‐8579 JapanDepartment of Biomolecular Engineering Graduate School of Engineering Tohoku University Aobayama 6‐6‐07 Sendai 980‐8579 JapanDepartment of Biosciences University of Milan Via G. Celoria 26 Milan 20133 ItalyDepartment of Biosciences University of Milan Via G. Celoria 26 Milan 20133 ItalyGraduate School of Environmental and Life Science Okayama University Tsushima Okayama 700‐8530 JapanGraduate School of Environmental and Life Science Okayama University Tsushima Okayama 700‐8530 JapanDepartment of Biomolecular Engineering Graduate School of Engineering Tohoku University Aobayama 6‐6‐07 Sendai 980‐8579 JapanDepartment of Biomolecular Engineering Graduate School of Engineering Tohoku University Aobayama 6‐6‐07 Sendai 980‐8579 JapanAbstract Stomatal movement is indispensable for plant growth and survival in response to environmental stimuli. Cytosolic Ca2+ elevation plays a crucial role in ABA‐induced stomatal closure during drought stress; however, to what extent the Ca2+ movement across the plasma membrane from the apoplast to the cytosol contributes to this process still needs clarification. Here the authors identify (−)‐catechin gallate (CG) and (−)‐gallocatechin gallate (GCG), components of green tea, as inhibitors of voltage‐dependent K+ channels which regulate K+ fluxes in Arabidopsis thaliana guard cells. In Arabidopsis guard cells CG/GCG prevent ABA‐induced: i) membrane depolarization; ii) activation of Ca2+ permeable cation (ICa) channels; and iii) cytosolic Ca2+ transients. In whole Arabidopsis plants co‐treatment with CG/GCG and ABA suppressed ABA‐induced stomatal closure and surface temperature increase. Similar to ABA, CG/GCG inhibited stomatal closure is elicited by the elicitor peptide, flg22 but has no impact on dark‐induced stomatal closure or light‐ and fusicoccin‐induced stomatal opening, suggesting that the inhibitory effect of CG/GCG is associated with Ca2+‐related signaling pathways. This study further supports the crucial role of ICa channels of the plasma membrane in ABA‐induced stomatal closure. Moreover, CG and GCG represent a new tool for the study of abiotic or biotic stress‐induced signal transduction pathways.https://doi.org/10.1002/advs.202201403calcium oscillationcatechin gallatedrought stressgreen teastomata |
| spellingShingle | Kanane Sato Shunya Saito Kohsuke Endo Masaru Kono Taishin Kakei Haruka Taketa Megumi Kato Shin Hamamoto Matteo Grenzi Alex Costa Shintaro Munemasa Yoshiyuki Murata Yasuhiro Ishimaru Nobuyuki Uozumi Green Tea Catechins, (−)‐Catechin Gallate, and (−)‐Gallocatechin Gallate are Potent Inhibitors of ABA‐Induced Stomatal Closure Advanced Science calcium oscillation catechin gallate drought stress green tea stomata |
| title | Green Tea Catechins, (−)‐Catechin Gallate, and (−)‐Gallocatechin Gallate are Potent Inhibitors of ABA‐Induced Stomatal Closure |
| title_full | Green Tea Catechins, (−)‐Catechin Gallate, and (−)‐Gallocatechin Gallate are Potent Inhibitors of ABA‐Induced Stomatal Closure |
| title_fullStr | Green Tea Catechins, (−)‐Catechin Gallate, and (−)‐Gallocatechin Gallate are Potent Inhibitors of ABA‐Induced Stomatal Closure |
| title_full_unstemmed | Green Tea Catechins, (−)‐Catechin Gallate, and (−)‐Gallocatechin Gallate are Potent Inhibitors of ABA‐Induced Stomatal Closure |
| title_short | Green Tea Catechins, (−)‐Catechin Gallate, and (−)‐Gallocatechin Gallate are Potent Inhibitors of ABA‐Induced Stomatal Closure |
| title_sort | green tea catechins catechin gallate and gallocatechin gallate are potent inhibitors of aba induced stomatal closure |
| topic | calcium oscillation catechin gallate drought stress green tea stomata |
| url | https://doi.org/10.1002/advs.202201403 |
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