Effects of adding Sophora alopecuroides to high concentrate diet on rumen fermentation parameters and microbial diversity of sheep
ObjectiveThe purpose of this study was to investigate the effects of different doses of Sophora alopecuroides (SA) on the rumen fermentation and microbial diversity of sheep.MethodsA total of 32 healthy Dumont crossbred male lambs weighing 25.73 ± 2.17 kg were randomly assigned to 4 treatment groups...
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Frontiers Media S.A.
2023-08-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2023.1200272/full |
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| author | Yawen An Hairong Wang Hairong Wang Zichao Zong Zhixiong Gao Caixia Shi Caixia Shi Shufang Li Khas-Erdene Khas-Erdene |
| author_facet | Yawen An Hairong Wang Hairong Wang Zichao Zong Zhixiong Gao Caixia Shi Caixia Shi Shufang Li Khas-Erdene Khas-Erdene |
| author_sort | Yawen An |
| collection | DOAJ |
| description | ObjectiveThe purpose of this study was to investigate the effects of different doses of Sophora alopecuroides (SA) on the rumen fermentation and microbial diversity of sheep.MethodsA total of 32 healthy Dumont crossbred male lambs weighing 25.73 ± 2.17 kg were randomly assigned to 4 treatment groups with 8 replicates each: a control group (CG) fed a basal diet with a concentrate-to-forage ratio of 7:3 and three experimental groups - the 0.1% group(TG1), 0.3% group (TG2), and 0.5% group (TG3), which were fed the same basal diet but supplemented with increasing doses of SA.Results(1) Increasing the SA dose led to a significant linear increase (p-< 0.05) in acetate, propionate, butyrate, and total volatile fatty acid (TVFA) concentrations in the rumen, as well as a significant quadratic effect (p-< 0.05) on the propionate concentration. In contrast, there was a significant linear decrease (p-< 0.05) in the NH3-N concentration in the rumen. (2) At the level of rumen bacterial phyla, the abundance of Bacteroidetes in the rumen increased, and that of Firmicutes decreased (p = 0.08). At the genus level, the rumen abundances of Ruminococcus and Phocaeicola of sheep in the three experimental groups were significantly higher than in the control group (p-< 0.05), and the abundances of Clostridiales and Candidatus-Hepatincola were significantly increased in the 0.1% and 0.3% groups (p < 0.05). (3) Regarding rumen anaerobic fungi, the differences between the control group and experimental groups at the phylum level and genus level were not significant (p > 0.05), but the relative abundances of Neocallimastigomycota and Piromyces in the 0.1% group were significantly higher than that in the control group.ConclusionSA addition to a high grain diet could increase the VFA concentration and pH in the sheep rumen, reduce the NH3-N concentration in the rumen and improve rumen fermentation function. Although there was no significant change in rumen bacterial or fungal diversity, SA addition increased the rumen abundances of Bacteroidetes, Ruminococcus, Phocaeicola, Clostridiales, Neocallimastigomycota and Piromyces, decreased the rumen abundance of Firmicutes, and had a positive effect on the rumen microbiota to improve sheep health. |
| first_indexed | 2024-03-12T17:00:15Z |
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| id | doaj.art-df4b1af4ee114f15b8b8a623cfc7f719 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-03-12T17:00:15Z |
| publishDate | 2023-08-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Veterinary Science |
| spelling | doaj.art-df4b1af4ee114f15b8b8a623cfc7f7192023-08-07T15:44:29ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692023-08-011010.3389/fvets.2023.12002721200272Effects of adding Sophora alopecuroides to high concentrate diet on rumen fermentation parameters and microbial diversity of sheepYawen An0Hairong Wang1Hairong Wang2Zichao Zong3Zhixiong Gao4Caixia Shi5Caixia Shi6Shufang Li7 Khas-Erdene8 Khas-Erdene9College of Animal Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, ChinaKey Laboratory of Animal Nutrition, Animal Nutrition and Feed Science, Hohhot, Inner Mongolia, ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, ChinaKey Laboratory of Animal Nutrition, Animal Nutrition and Feed Science, Hohhot, Inner Mongolia, ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, ChinaKey Laboratory of Animal Nutrition, Animal Nutrition and Feed Science, Hohhot, Inner Mongolia, ChinaObjectiveThe purpose of this study was to investigate the effects of different doses of Sophora alopecuroides (SA) on the rumen fermentation and microbial diversity of sheep.MethodsA total of 32 healthy Dumont crossbred male lambs weighing 25.73 ± 2.17 kg were randomly assigned to 4 treatment groups with 8 replicates each: a control group (CG) fed a basal diet with a concentrate-to-forage ratio of 7:3 and three experimental groups - the 0.1% group(TG1), 0.3% group (TG2), and 0.5% group (TG3), which were fed the same basal diet but supplemented with increasing doses of SA.Results(1) Increasing the SA dose led to a significant linear increase (p-< 0.05) in acetate, propionate, butyrate, and total volatile fatty acid (TVFA) concentrations in the rumen, as well as a significant quadratic effect (p-< 0.05) on the propionate concentration. In contrast, there was a significant linear decrease (p-< 0.05) in the NH3-N concentration in the rumen. (2) At the level of rumen bacterial phyla, the abundance of Bacteroidetes in the rumen increased, and that of Firmicutes decreased (p = 0.08). At the genus level, the rumen abundances of Ruminococcus and Phocaeicola of sheep in the three experimental groups were significantly higher than in the control group (p-< 0.05), and the abundances of Clostridiales and Candidatus-Hepatincola were significantly increased in the 0.1% and 0.3% groups (p < 0.05). (3) Regarding rumen anaerobic fungi, the differences between the control group and experimental groups at the phylum level and genus level were not significant (p > 0.05), but the relative abundances of Neocallimastigomycota and Piromyces in the 0.1% group were significantly higher than that in the control group.ConclusionSA addition to a high grain diet could increase the VFA concentration and pH in the sheep rumen, reduce the NH3-N concentration in the rumen and improve rumen fermentation function. Although there was no significant change in rumen bacterial or fungal diversity, SA addition increased the rumen abundances of Bacteroidetes, Ruminococcus, Phocaeicola, Clostridiales, Neocallimastigomycota and Piromyces, decreased the rumen abundance of Firmicutes, and had a positive effect on the rumen microbiota to improve sheep health.https://www.frontiersin.org/articles/10.3389/fvets.2023.1200272/fullSophora alopecuroideshigh concentrate dietrumen fermentationrumen bacteriarumen fungilambs |
| spellingShingle | Yawen An Hairong Wang Hairong Wang Zichao Zong Zhixiong Gao Caixia Shi Caixia Shi Shufang Li Khas-Erdene Khas-Erdene Effects of adding Sophora alopecuroides to high concentrate diet on rumen fermentation parameters and microbial diversity of sheep Frontiers in Veterinary Science Sophora alopecuroides high concentrate diet rumen fermentation rumen bacteria rumen fungi lambs |
| title | Effects of adding Sophora alopecuroides to high concentrate diet on rumen fermentation parameters and microbial diversity of sheep |
| title_full | Effects of adding Sophora alopecuroides to high concentrate diet on rumen fermentation parameters and microbial diversity of sheep |
| title_fullStr | Effects of adding Sophora alopecuroides to high concentrate diet on rumen fermentation parameters and microbial diversity of sheep |
| title_full_unstemmed | Effects of adding Sophora alopecuroides to high concentrate diet on rumen fermentation parameters and microbial diversity of sheep |
| title_short | Effects of adding Sophora alopecuroides to high concentrate diet on rumen fermentation parameters and microbial diversity of sheep |
| title_sort | effects of adding sophora alopecuroides to high concentrate diet on rumen fermentation parameters and microbial diversity of sheep |
| topic | Sophora alopecuroides high concentrate diet rumen fermentation rumen bacteria rumen fungi lambs |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2023.1200272/full |
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