Within-Household Transmission and Bacterial Diversity of <i>Staphylococcus pseudintermedius</i>
<i>Staphylococcus pseudintermedius</i> can be transmitted between dogs and their owners and can cause opportunistic infections in humans. Whole genome sequencing was applied to identify the relatedness between isolates from human infections and isolates from dogs in the same households....
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-07-01
|
| Series: | Pathogens |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-0817/11/8/850 |
| _version_ | 1797408532729954304 |
|---|---|
| author | Alice Wegener Birgitta Duim Linda van der Graaf-van Bloois Aldert L. Zomer Caroline E. Visser Mirlin Spaninks Arjen J. Timmerman Jaap A. Wagenaar Els M. Broens |
| author_facet | Alice Wegener Birgitta Duim Linda van der Graaf-van Bloois Aldert L. Zomer Caroline E. Visser Mirlin Spaninks Arjen J. Timmerman Jaap A. Wagenaar Els M. Broens |
| author_sort | Alice Wegener |
| collection | DOAJ |
| description | <i>Staphylococcus pseudintermedius</i> can be transmitted between dogs and their owners and can cause opportunistic infections in humans. Whole genome sequencing was applied to identify the relatedness between isolates from human infections and isolates from dogs in the same households. Genome SNP diversity and distribution of plasmids and antimicrobial resistance genes identified related and unrelated isolates in both households. Our study shows that within-host bacterial diversity is present in <i>S. pseudintermedius</i>, demonstrating that multiple isolates from each host should preferably be sequenced to study transmission dynamics. |
| first_indexed | 2024-03-09T03:59:54Z |
| format | Article |
| id | doaj.art-df51a70510df40b2b14ce3e059cc96b1 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T03:59:54Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-df51a70510df40b2b14ce3e059cc96b12023-12-03T14:15:17ZengMDPI AGPathogens2076-08172022-07-0111885010.3390/pathogens11080850Within-Household Transmission and Bacterial Diversity of <i>Staphylococcus pseudintermedius</i>Alice Wegener0Birgitta Duim1Linda van der Graaf-van Bloois2Aldert L. Zomer3Caroline E. Visser4Mirlin Spaninks5Arjen J. Timmerman6Jaap A. Wagenaar7Els M. Broens8Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CL Utrecht, The NetherlandsDepartment of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CL Utrecht, The NetherlandsDepartment of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CL Utrecht, The NetherlandsDepartment of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CL Utrecht, The NetherlandsDepartment of Medical Microbiology & Infection Prevention, Amsterdam UMC Location AMC, Amsterdam, 1105 AZ Amsterdam, The NetherlandsDepartment Population Health Sciences, Faculty of Veterinary Medicine, Utrecht University, 3584 CL Utrecht, The NetherlandsDepartment of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CL Utrecht, The NetherlandsDepartment of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CL Utrecht, The NetherlandsDepartment of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CL Utrecht, The Netherlands<i>Staphylococcus pseudintermedius</i> can be transmitted between dogs and their owners and can cause opportunistic infections in humans. Whole genome sequencing was applied to identify the relatedness between isolates from human infections and isolates from dogs in the same households. Genome SNP diversity and distribution of plasmids and antimicrobial resistance genes identified related and unrelated isolates in both households. Our study shows that within-host bacterial diversity is present in <i>S. pseudintermedius</i>, demonstrating that multiple isolates from each host should preferably be sequenced to study transmission dynamics.https://www.mdpi.com/2076-0817/11/8/850<i>S. pseudintermedius</i>transmissionOne healthwhole genome sequencingzoonoticbacterial diversity |
| spellingShingle | Alice Wegener Birgitta Duim Linda van der Graaf-van Bloois Aldert L. Zomer Caroline E. Visser Mirlin Spaninks Arjen J. Timmerman Jaap A. Wagenaar Els M. Broens Within-Household Transmission and Bacterial Diversity of <i>Staphylococcus pseudintermedius</i> Pathogens <i>S. pseudintermedius</i> transmission One health whole genome sequencing zoonotic bacterial diversity |
| title | Within-Household Transmission and Bacterial Diversity of <i>Staphylococcus pseudintermedius</i> |
| title_full | Within-Household Transmission and Bacterial Diversity of <i>Staphylococcus pseudintermedius</i> |
| title_fullStr | Within-Household Transmission and Bacterial Diversity of <i>Staphylococcus pseudintermedius</i> |
| title_full_unstemmed | Within-Household Transmission and Bacterial Diversity of <i>Staphylococcus pseudintermedius</i> |
| title_short | Within-Household Transmission and Bacterial Diversity of <i>Staphylococcus pseudintermedius</i> |
| title_sort | within household transmission and bacterial diversity of i staphylococcus pseudintermedius i |
| topic | <i>S. pseudintermedius</i> transmission One health whole genome sequencing zoonotic bacterial diversity |
| url | https://www.mdpi.com/2076-0817/11/8/850 |
| work_keys_str_mv | AT alicewegener withinhouseholdtransmissionandbacterialdiversityofistaphylococcuspseudintermediusi AT birgittaduim withinhouseholdtransmissionandbacterialdiversityofistaphylococcuspseudintermediusi AT lindavandergraafvanbloois withinhouseholdtransmissionandbacterialdiversityofistaphylococcuspseudintermediusi AT aldertlzomer withinhouseholdtransmissionandbacterialdiversityofistaphylococcuspseudintermediusi AT carolineevisser withinhouseholdtransmissionandbacterialdiversityofistaphylococcuspseudintermediusi AT mirlinspaninks withinhouseholdtransmissionandbacterialdiversityofistaphylococcuspseudintermediusi AT arjenjtimmerman withinhouseholdtransmissionandbacterialdiversityofistaphylococcuspseudintermediusi AT jaapawagenaar withinhouseholdtransmissionandbacterialdiversityofistaphylococcuspseudintermediusi AT elsmbroens withinhouseholdtransmissionandbacterialdiversityofistaphylococcuspseudintermediusi |