Multiplex Real Time PCR Test-System for the Detection of Anthrax Causative Agent
Designed is a multiplex PCR-RT test-system for the detection of anthrax causative agent using hybridization fluorescence detection – “ Bacillus anthracis multiplex 3 FRT”. This test-system contains enclosed reference sample for carrying out control procedure in the phase of DNA pur...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | Russian |
| Published: |
Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”
2013-03-01
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| Series: | Проблемы особо опасных инфекций |
| Subjects: | |
| Online Access: | https://journal.microbe.ru/jour/article/view/17 |
| _version_ | 1826922468955127808 |
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| author | E. A. Tsygankova E. I. Eremenko A. G. Ryazanova O. I. Tsygankova A. N. Kulichenko |
| author_facet | E. A. Tsygankova E. I. Eremenko A. G. Ryazanova O. I. Tsygankova A. N. Kulichenko |
| author_sort | E. A. Tsygankova |
| collection | DOAJ |
| description | Designed is a multiplex PCR-RT test-system for the detection of anthrax causative agent using hybridization fluorescence detection – “ Bacillus anthracis multiplex 3 FRT”. This test-system contains enclosed reference sample for carrying out control procedure in the phase of DNA purification, primers and probes to specific DNA target sequences of the two plasmid and one chromosomal B. anthracis genes. It allows for identification B. anthracis strains both possessing plasmids (one or two) and plasmidless, revealing their genetic pathogenicity potential, and for differentiation between anthrax agent strains and closely related saprophytes belonging to Bacillus species. |
| first_indexed | 2024-03-08T13:43:54Z |
| format | Article |
| id | doaj.art-df52e3e45e0c465aa1873d04a5aaa6d7 |
| institution | Directory Open Access Journal |
| issn | 0370-1069 2658-719X |
| language | Russian |
| last_indexed | 2025-02-17T14:10:14Z |
| publishDate | 2013-03-01 |
| publisher | Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” |
| record_format | Article |
| series | Проблемы особо опасных инфекций |
| spelling | doaj.art-df52e3e45e0c465aa1873d04a5aaa6d72024-12-24T12:09:49ZrusFederal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”Проблемы особо опасных инфекций0370-10692658-719X2013-03-0101818410.21055/0370-1069-2013-1-81-8417Multiplex Real Time PCR Test-System for the Detection of Anthrax Causative AgentE. A. Tsygankova0E. I. Eremenko1A. G. Ryazanova2O. I. Tsygankova3A. N. Kulichenko4Stavropol Research Anti-Plague InstituteStavropol Research Anti-Plague InstituteStavropol Research Anti-Plague InstituteStavropol Research Anti-Plague InstituteStavropol Research Anti-Plague InstituteDesigned is a multiplex PCR-RT test-system for the detection of anthrax causative agent using hybridization fluorescence detection – “ Bacillus anthracis multiplex 3 FRT”. This test-system contains enclosed reference sample for carrying out control procedure in the phase of DNA purification, primers and probes to specific DNA target sequences of the two plasmid and one chromosomal B. anthracis genes. It allows for identification B. anthracis strains both possessing plasmids (one or two) and plasmidless, revealing their genetic pathogenicity potential, and for differentiation between anthrax agent strains and closely related saprophytes belonging to Bacillus species.https://journal.microbe.ru/jour/article/view/17bacillus anthracisтест-системадетекцияпцрtest-systemdetectionpcr |
| spellingShingle | E. A. Tsygankova E. I. Eremenko A. G. Ryazanova O. I. Tsygankova A. N. Kulichenko Multiplex Real Time PCR Test-System for the Detection of Anthrax Causative Agent Проблемы особо опасных инфекций bacillus anthracis тест-система детекция пцр test-system detection pcr |
| title | Multiplex Real Time PCR Test-System for the Detection of Anthrax Causative Agent |
| title_full | Multiplex Real Time PCR Test-System for the Detection of Anthrax Causative Agent |
| title_fullStr | Multiplex Real Time PCR Test-System for the Detection of Anthrax Causative Agent |
| title_full_unstemmed | Multiplex Real Time PCR Test-System for the Detection of Anthrax Causative Agent |
| title_short | Multiplex Real Time PCR Test-System for the Detection of Anthrax Causative Agent |
| title_sort | multiplex real time pcr test system for the detection of anthrax causative agent |
| topic | bacillus anthracis тест-система детекция пцр test-system detection pcr |
| url | https://journal.microbe.ru/jour/article/view/17 |
| work_keys_str_mv | AT eatsygankova multiplexrealtimepcrtestsystemforthedetectionofanthraxcausativeagent AT eieremenko multiplexrealtimepcrtestsystemforthedetectionofanthraxcausativeagent AT agryazanova multiplexrealtimepcrtestsystemforthedetectionofanthraxcausativeagent AT oitsygankova multiplexrealtimepcrtestsystemforthedetectionofanthraxcausativeagent AT ankulichenko multiplexrealtimepcrtestsystemforthedetectionofanthraxcausativeagent |