The Effects of Bioprocess Parameters on Cellulase Production with Trichoderma viride CMIT35
Fungal cellulases are well-studied, and have various applications in industry, health or agriculture. Species of Trichoderma can produce substantial amounts of endoglucanase, exoglucanase (saccharifying cellulases), and some strains are able to produce important quantities of β-glucosidase. A number...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Agroprint Timisoara
2023-10-01
|
| Series: | Scientific Papers Animal Science and Biotechnologies |
| Subjects: | |
| Online Access: | https://spasb.ro/index.php/public_html/article/view/1281 |
| _version_ | 1797215655989084160 |
|---|---|
| author | Teodor Vintilă Veronica Croitoru Monica Dragomirescu Dragos Nica |
| author_facet | Teodor Vintilă Veronica Croitoru Monica Dragomirescu Dragos Nica |
| author_sort | Teodor Vintilă |
| collection | DOAJ |
| description | Fungal cellulases are well-studied, and have various applications in industry, health or agriculture. Species of Trichoderma can produce substantial amounts of endoglucanase, exoglucanase (saccharifying cellulases), and some strains are able to produce important quantities of β-glucosidase. A number of fungi were isolated abroad and screened for cellulolytic potential. In this study, the kinetics of cellulase production from an indigenous strain of T. viride CMIT35 is reported. Product formation parameters of different types of cellulases indicate that the studied strain of T. viride is capable of producing important levels of cellulases when grown on Mandels medium with wheat bran as carbon source. Furthermore, it was observed that production of endoglucanase reaches its maximum during exponential phase of growth, while exoglucanase during the stationary phase. Enzyme production by solid-state fermentation was also investigated and found to be more efficient than liquid state fermentation. High production of cellulase was noted at the following parameters for liquid cultures: 4% wheat bran, 5% inoculum, 180 r.p.m. agitation, pH 5; and 60% humidity in the case of solid state fermentation. |
| first_indexed | 2024-04-24T11:33:32Z |
| format | Article |
| id | doaj.art-df532bb90e72455fa4a2549662108d00 |
| institution | Directory Open Access Journal |
| issn | 1841-9364 2344-4576 |
| language | English |
| last_indexed | 2024-04-24T11:33:32Z |
| publishDate | 2023-10-01 |
| publisher | Agroprint Timisoara |
| record_format | Article |
| series | Scientific Papers Animal Science and Biotechnologies |
| spelling | doaj.art-df532bb90e72455fa4a2549662108d002024-04-10T08:10:45ZengAgroprint TimisoaraScientific Papers Animal Science and Biotechnologies1841-93642344-45762023-10-014313373371281The Effects of Bioprocess Parameters on Cellulase Production with Trichoderma viride CMIT35Teodor Vintilă0Veronica Croitoru1Monica Dragomirescu2Dragos Nica3USAMVB, Faculty of Animal Science and Biotechnology, Timisoara 300645, Calea Aradului 119, RomaniaUSAMVB, Faculty of Animal Science and Biotechnology, Timisoara 300645, Calea Aradului 119, RomaniaUSAMVB, Faculty of Animal Science and Biotechnology, Timisoara 300645, Calea Aradului 119, RomaniaUSAMVB, Faculty of Animal Science and Biotechnology, Timisoara 300645, Calea Aradului 119, RomaniaFungal cellulases are well-studied, and have various applications in industry, health or agriculture. Species of Trichoderma can produce substantial amounts of endoglucanase, exoglucanase (saccharifying cellulases), and some strains are able to produce important quantities of β-glucosidase. A number of fungi were isolated abroad and screened for cellulolytic potential. In this study, the kinetics of cellulase production from an indigenous strain of T. viride CMIT35 is reported. Product formation parameters of different types of cellulases indicate that the studied strain of T. viride is capable of producing important levels of cellulases when grown on Mandels medium with wheat bran as carbon source. Furthermore, it was observed that production of endoglucanase reaches its maximum during exponential phase of growth, while exoglucanase during the stationary phase. Enzyme production by solid-state fermentation was also investigated and found to be more efficient than liquid state fermentation. High production of cellulase was noted at the following parameters for liquid cultures: 4% wheat bran, 5% inoculum, 180 r.p.m. agitation, pH 5; and 60% humidity in the case of solid state fermentation.https://spasb.ro/index.php/public_html/article/view/1281cellulaseculture parameterstrichoderma viride |
| spellingShingle | Teodor Vintilă Veronica Croitoru Monica Dragomirescu Dragos Nica The Effects of Bioprocess Parameters on Cellulase Production with Trichoderma viride CMIT35 Scientific Papers Animal Science and Biotechnologies cellulase culture parameters trichoderma viride |
| title | The Effects of Bioprocess Parameters on Cellulase Production with Trichoderma viride CMIT35 |
| title_full | The Effects of Bioprocess Parameters on Cellulase Production with Trichoderma viride CMIT35 |
| title_fullStr | The Effects of Bioprocess Parameters on Cellulase Production with Trichoderma viride CMIT35 |
| title_full_unstemmed | The Effects of Bioprocess Parameters on Cellulase Production with Trichoderma viride CMIT35 |
| title_short | The Effects of Bioprocess Parameters on Cellulase Production with Trichoderma viride CMIT35 |
| title_sort | effects of bioprocess parameters on cellulase production with trichoderma viride cmit35 |
| topic | cellulase culture parameters trichoderma viride |
| url | https://spasb.ro/index.php/public_html/article/view/1281 |
| work_keys_str_mv | AT teodorvintila theeffectsofbioprocessparametersoncellulaseproductionwithtrichodermaviridecmit35 AT veronicacroitoru theeffectsofbioprocessparametersoncellulaseproductionwithtrichodermaviridecmit35 AT monicadragomirescu theeffectsofbioprocessparametersoncellulaseproductionwithtrichodermaviridecmit35 AT dragosnica theeffectsofbioprocessparametersoncellulaseproductionwithtrichodermaviridecmit35 AT teodorvintila effectsofbioprocessparametersoncellulaseproductionwithtrichodermaviridecmit35 AT veronicacroitoru effectsofbioprocessparametersoncellulaseproductionwithtrichodermaviridecmit35 AT monicadragomirescu effectsofbioprocessparametersoncellulaseproductionwithtrichodermaviridecmit35 AT dragosnica effectsofbioprocessparametersoncellulaseproductionwithtrichodermaviridecmit35 |