In vitro antioxidant and cell viability of Pyrostegia venusta (Ker Gawl.) Miers)
Many diseases are associated with oxidative stress and inflammatory processes. The current research is directed toward evaluating the antioxidant potential and phytochemistry composition of P. venusta leaves. In this study, P. venusta leaves were dried and macerated, and the crude extract was partit...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universidade Federal de Mato Grosso do Sul
2015-01-01
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| Series: | Orbital: The Electronic Journal of Chemistry |
| Subjects: | |
| Online Access: | http://orbital.ufms.br/index.php/Chemistry/article/view/603 |
| _version_ | 1818976873194979328 |
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| author | Thales D. P. Altoé Gustavo M. de Amorim João Victor D. Gomes Augusto S. Borges Iuri C. Valadão Ian Victor Silva Letícia B. A. Rangel Paulo Cezar Vieira Claudia M. Jamal Rodrigo R. Kitagawa Warley de Souza Borges |
| author_facet | Thales D. P. Altoé Gustavo M. de Amorim João Victor D. Gomes Augusto S. Borges Iuri C. Valadão Ian Victor Silva Letícia B. A. Rangel Paulo Cezar Vieira Claudia M. Jamal Rodrigo R. Kitagawa Warley de Souza Borges |
| author_sort | Thales D. P. Altoé |
| collection | DOAJ |
| description | Many diseases are associated with oxidative stress and inflammatory processes. The current research is directed toward evaluating the antioxidant potential and phytochemistry composition of P. venusta leaves. In this study, P. venusta leaves were dried and macerated, and the crude extract was partitioned. Phytochemical analysis was performed using standard methodologies, and the total flavonoid content was measured using a calibration curve with rutin. We evaluated the antioxidant potential of P. venusta leaves using 1,1-Diphenyl-2-picrylhydrazyl (DPPH), 2, 2’-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), and a Trolox-like standard. Cell viability<em> </em>(CV) assays were done using macrophage RAW 264.7 cell lines and compared to four commercial anti-inflammatories (acetylsalicylic acid, Indometacina, Betametasona, and Piroxicam). Phytochemical analysis revealed the presence of steroids, coumarins, and flavone. The flavonoid content was 148.5 ± 7.65 µg as a rutin equivalent/mg of crude extract. The ethyl acetate fraction showed the best antioxidant activity in the methodologies of DPPH inhibition (IC<sub>50 </sub>= 38.62 <em>µ</em>g/mL) and ABTS radical (IC<sub>50 </sub>= 28.58 <em>µ</em>g/mL). Samples of P. venusta had CV values that were better than the commercial anti-inflammatory, which showed CV values below the negative control. The crude extract and the ethyl acetate fraction, showed CV values below the negative control<em> </em>and the hexane fraction obtained values above the negative control, these being best results<em>.</em> |
| first_indexed | 2024-12-20T16:18:46Z |
| format | Article |
| id | doaj.art-df554b1624dd4d5cb213b6780ef1a869 |
| institution | Directory Open Access Journal |
| issn | 1984-6428 |
| language | English |
| last_indexed | 2024-12-20T16:18:46Z |
| publishDate | 2015-01-01 |
| publisher | Universidade Federal de Mato Grosso do Sul |
| record_format | Article |
| series | Orbital: The Electronic Journal of Chemistry |
| spelling | doaj.art-df554b1624dd4d5cb213b6780ef1a8692022-12-21T19:33:42ZengUniversidade Federal de Mato Grosso do SulOrbital: The Electronic Journal of Chemistry1984-64282015-01-016421522210.17807/orbital.v6i4.603298In vitro antioxidant and cell viability of Pyrostegia venusta (Ker Gawl.) Miers)Thales D. P. Altoé0Gustavo M. de Amorim1João Victor D. Gomes2Augusto S. Borges3Iuri C. Valadão4Ian Victor Silva5Letícia B. A. Rangel6Paulo Cezar Vieira7Claudia M. Jamal8Rodrigo R. Kitagawa9Warley de Souza Borges10Departamento de Química, Universidade Federal do Espírito SantoDepartamento de Ciências Farmacêuticas, Universidade Federal do Espírito SantoDepartamento de Ciências Farmacêuticas, Universidade Federal do Espírito SantoDepartamento de Ciências Farmacêuticas, Universidade Federal do Espírito SantoDepartamento de Ciências Farmacêuticas, Universidade Federal do Espírito SantoDepartamento de Morfologia, Centro de Ciências da Saúde, Universidade Federal do Espírito SantoDepartamento de Ciências Farmacêuticas, Universidade Federal do Espírito SantoDepartamento de Química, Universidade Federal de São CarlosDepartamento de Ciências Farmacêuticas, Universidade Federal do Espírito SantoDepartamento de Ciências Farmacêuticas, Universidade Federal do Espírito SantoUniversidade Federal do Espírito SantoMany diseases are associated with oxidative stress and inflammatory processes. The current research is directed toward evaluating the antioxidant potential and phytochemistry composition of P. venusta leaves. In this study, P. venusta leaves were dried and macerated, and the crude extract was partitioned. Phytochemical analysis was performed using standard methodologies, and the total flavonoid content was measured using a calibration curve with rutin. We evaluated the antioxidant potential of P. venusta leaves using 1,1-Diphenyl-2-picrylhydrazyl (DPPH), 2, 2’-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), and a Trolox-like standard. Cell viability<em> </em>(CV) assays were done using macrophage RAW 264.7 cell lines and compared to four commercial anti-inflammatories (acetylsalicylic acid, Indometacina, Betametasona, and Piroxicam). Phytochemical analysis revealed the presence of steroids, coumarins, and flavone. The flavonoid content was 148.5 ± 7.65 µg as a rutin equivalent/mg of crude extract. The ethyl acetate fraction showed the best antioxidant activity in the methodologies of DPPH inhibition (IC<sub>50 </sub>= 38.62 <em>µ</em>g/mL) and ABTS radical (IC<sub>50 </sub>= 28.58 <em>µ</em>g/mL). Samples of P. venusta had CV values that were better than the commercial anti-inflammatory, which showed CV values below the negative control. The crude extract and the ethyl acetate fraction, showed CV values below the negative control<em> </em>and the hexane fraction obtained values above the negative control, these being best results<em>.</em>http://orbital.ufms.br/index.php/Chemistry/article/view/603pyrostegia venustaleavesantioxidantdpphabtscell viability. |
| spellingShingle | Thales D. P. Altoé Gustavo M. de Amorim João Victor D. Gomes Augusto S. Borges Iuri C. Valadão Ian Victor Silva Letícia B. A. Rangel Paulo Cezar Vieira Claudia M. Jamal Rodrigo R. Kitagawa Warley de Souza Borges In vitro antioxidant and cell viability of Pyrostegia venusta (Ker Gawl.) Miers) Orbital: The Electronic Journal of Chemistry pyrostegia venusta leaves antioxidant dpph abts cell viability. |
| title | In vitro antioxidant and cell viability of Pyrostegia venusta (Ker Gawl.) Miers) |
| title_full | In vitro antioxidant and cell viability of Pyrostegia venusta (Ker Gawl.) Miers) |
| title_fullStr | In vitro antioxidant and cell viability of Pyrostegia venusta (Ker Gawl.) Miers) |
| title_full_unstemmed | In vitro antioxidant and cell viability of Pyrostegia venusta (Ker Gawl.) Miers) |
| title_short | In vitro antioxidant and cell viability of Pyrostegia venusta (Ker Gawl.) Miers) |
| title_sort | in vitro antioxidant and cell viability of pyrostegia venusta ker gawl miers |
| topic | pyrostegia venusta leaves antioxidant dpph abts cell viability. |
| url | http://orbital.ufms.br/index.php/Chemistry/article/view/603 |
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