Effect of exogenous human chorionic gonadotropin on ovulation in mice
The implementation of assisted reproductive technologies (ART), hormonal stimulation in particular, may change the quality of ovulated oocytes. The purpose of our work was to study ovulation in CD1 mice after their stimulation with human chorionic gonadotropin (hCG) and to investigate the effects of...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2020-01-01
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| Series: | Вавиловский журнал генетики и селекции |
| Subjects: | |
| Online Access: | https://vavilov.elpub.ru/jour/article/view/2394 |
| _version_ | 1797214042423558144 |
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| author | S. Ya. Amstislavsky S. V. Ranneva D. S. Ragaeva E. A. Chuyko A. M. Popkova E. Yu. Brusentsev |
| author_facet | S. Ya. Amstislavsky S. V. Ranneva D. S. Ragaeva E. A. Chuyko A. M. Popkova E. Yu. Brusentsev |
| author_sort | S. Ya. Amstislavsky |
| collection | DOAJ |
| description | The implementation of assisted reproductive technologies (ART), hormonal stimulation in particular, may change the quality of ovulated oocytes. The purpose of our work was to study ovulation in CD1 mice after their stimulation with human chorionic gonadotropin (hCG) and to investigate the effects of such hormonal stimulation on the pregnancy duration, fetal losses and the weight of the offspring. No significant differences were found in the total number of ovulated oocytes or in the number of immature (without a polar body) ovulated oocytes; nor were there differences between the groups in the number of oocytes with a developing polar body. However, the number of matured oocytes with a distinct polar body was significantly higher (p < 0.05) in mice stimulated with hCG (experimental group) as compared with the controls (6.2 ± 0.86 and 2.2 ± 0.97, respectively). No significant differences were observed between the experimental and control mice in the duration of pregnancy or in the numbers of term offspring, including the percentage of live and stillborn pups. However, the body weight of the offspring in the experimental group was significantly lower (p < 0.001) as compared with the controls on the fifth day after birth (3.16 ± 0.09 and 3.76 ± 0.07, respectively). Thus, exogenous hCG facilitates the development of mouse oocytes in vivo, which leads to the larger number of their mature forms at ovulation, however, the offspring born after hCG-stimulated pregnancy was characterized by a lower body weight on the fifth day after birth. |
| first_indexed | 2024-03-07T16:05:33Z |
| format | Article |
| id | doaj.art-df5684b872a14bbe99269f7f09eaa85d |
| institution | Directory Open Access Journal |
| issn | 2500-3259 |
| language | English |
| last_indexed | 2024-04-24T11:07:53Z |
| publishDate | 2020-01-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj.art-df5684b872a14bbe99269f7f09eaa85d2024-04-11T15:31:02ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592020-01-012381006101010.18699/VJ19.5771002Effect of exogenous human chorionic gonadotropin on ovulation in miceS. Ya. Amstislavsky0S. V. Ranneva1D. S. Ragaeva2E. A. Chuyko3A. M. Popkova4E. Yu. Brusentsev5Institute of Cytology and Genetics, SB RAS; Novosibirsk State UniversityInstitute of Cytology and Genetics, SB RAS; Novosibirsk State UniversityInstitute of Cytology and Genetics, SB RASNovosibirsk State UniversityNovosibirsk State UniversityInstitute of Cytology and Genetics, SB RASThe implementation of assisted reproductive technologies (ART), hormonal stimulation in particular, may change the quality of ovulated oocytes. The purpose of our work was to study ovulation in CD1 mice after their stimulation with human chorionic gonadotropin (hCG) and to investigate the effects of such hormonal stimulation on the pregnancy duration, fetal losses and the weight of the offspring. No significant differences were found in the total number of ovulated oocytes or in the number of immature (without a polar body) ovulated oocytes; nor were there differences between the groups in the number of oocytes with a developing polar body. However, the number of matured oocytes with a distinct polar body was significantly higher (p < 0.05) in mice stimulated with hCG (experimental group) as compared with the controls (6.2 ± 0.86 and 2.2 ± 0.97, respectively). No significant differences were observed between the experimental and control mice in the duration of pregnancy or in the numbers of term offspring, including the percentage of live and stillborn pups. However, the body weight of the offspring in the experimental group was significantly lower (p < 0.001) as compared with the controls on the fifth day after birth (3.16 ± 0.09 and 3.76 ± 0.07, respectively). Thus, exogenous hCG facilitates the development of mouse oocytes in vivo, which leads to the larger number of their mature forms at ovulation, however, the offspring born after hCG-stimulated pregnancy was characterized by a lower body weight on the fifth day after birth.https://vavilov.elpub.ru/jour/article/view/2394miceoocyteshuman chorionic gonadotropin |
| spellingShingle | S. Ya. Amstislavsky S. V. Ranneva D. S. Ragaeva E. A. Chuyko A. M. Popkova E. Yu. Brusentsev Effect of exogenous human chorionic gonadotropin on ovulation in mice Вавиловский журнал генетики и селекции mice oocytes human chorionic gonadotropin |
| title | Effect of exogenous human chorionic gonadotropin on ovulation in mice |
| title_full | Effect of exogenous human chorionic gonadotropin on ovulation in mice |
| title_fullStr | Effect of exogenous human chorionic gonadotropin on ovulation in mice |
| title_full_unstemmed | Effect of exogenous human chorionic gonadotropin on ovulation in mice |
| title_short | Effect of exogenous human chorionic gonadotropin on ovulation in mice |
| title_sort | effect of exogenous human chorionic gonadotropin on ovulation in mice |
| topic | mice oocytes human chorionic gonadotropin |
| url | https://vavilov.elpub.ru/jour/article/view/2394 |
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