Microgels-on-macrogel: A simple cytophilic surface makeover of soft agarose substrates
Microgel-mediated surface modification has shown great promises for a variety of metallic and non-metallic substrates. Yet, despite its compelling merits, this approach is less implemented on soft hydrogel substrates. Here, using the well-known bioinert agarose hydrogel as an example, we highlight a...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-10-01
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| Series: | JCIS Open |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666934X22000149 |
| _version_ | 1818065796979490816 |
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| author | Xueying Yu Fang Cheng Wei He |
| author_facet | Xueying Yu Fang Cheng Wei He |
| author_sort | Xueying Yu |
| collection | DOAJ |
| description | Microgel-mediated surface modification has shown great promises for a variety of metallic and non-metallic substrates. Yet, despite its compelling merits, this approach is less implemented on soft hydrogel substrates. Here, using the well-known bioinert agarose hydrogel as an example, we highlight a microgels-on-macrogel strategy that readily confers cytophilicity to the agarose surface toward anchorage-dependent cells. Specifically, we selected glycerol diglycidyl ether to tailor design polyetheramine-bisepoxide-based cationic microgels with more prominent ether alcohol features for enhanced chemical compatibility with agarose. Through a simple drop casting method, concurrent modifications of chemical, morphological and mechanical properties of the surface of agarose gel were then achieved with these microgels bound to the surface in a non-covalent yet robust manner. With the mere introduction of the cationic microgels, not only was the non-adhesive agarose surface effectively transformed to be cytophilic shown by the favorable responses from the in vitro culture of MC3T3-E1 cells, but also was hydrophobic reservoir function integrated conveniently. The demonstration of its feasibility and versatility warrants continued research of this straightforward microgels-on-macrogel strategy, which could be of value particularly for the development of novel biointerfaces. |
| first_indexed | 2024-12-10T14:57:36Z |
| format | Article |
| id | doaj.art-df586cc1a3354c1d85efbaba152960f8 |
| institution | Directory Open Access Journal |
| issn | 2666-934X |
| language | English |
| last_indexed | 2024-12-10T14:57:36Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | JCIS Open |
| spelling | doaj.art-df586cc1a3354c1d85efbaba152960f82022-12-22T01:44:15ZengElsevierJCIS Open2666-934X2022-10-017100056Microgels-on-macrogel: A simple cytophilic surface makeover of soft agarose substratesXueying Yu0Fang Cheng1Wei He2State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian, Liaoning, 116023, China; School of Chemical Engineering, Dalian University of Technology, Dalian, Liaoning, 116023, ChinaState Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian, Liaoning, 116023, China; School of Chemical Engineering, Dalian University of Technology, Dalian, Liaoning, 116023, ChinaState Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian, Liaoning, 116023, China; School of Chemical Engineering, Dalian University of Technology, Dalian, Liaoning, 116023, China; Corresponding author. School of Chemical Engineering, Dalian University of Technology, Dalian, Liaoning, 116023, China.Microgel-mediated surface modification has shown great promises for a variety of metallic and non-metallic substrates. Yet, despite its compelling merits, this approach is less implemented on soft hydrogel substrates. Here, using the well-known bioinert agarose hydrogel as an example, we highlight a microgels-on-macrogel strategy that readily confers cytophilicity to the agarose surface toward anchorage-dependent cells. Specifically, we selected glycerol diglycidyl ether to tailor design polyetheramine-bisepoxide-based cationic microgels with more prominent ether alcohol features for enhanced chemical compatibility with agarose. Through a simple drop casting method, concurrent modifications of chemical, morphological and mechanical properties of the surface of agarose gel were then achieved with these microgels bound to the surface in a non-covalent yet robust manner. With the mere introduction of the cationic microgels, not only was the non-adhesive agarose surface effectively transformed to be cytophilic shown by the favorable responses from the in vitro culture of MC3T3-E1 cells, but also was hydrophobic reservoir function integrated conveniently. The demonstration of its feasibility and versatility warrants continued research of this straightforward microgels-on-macrogel strategy, which could be of value particularly for the development of novel biointerfaces.http://www.sciencedirect.com/science/article/pii/S2666934X22000149MicrogelColloidAgaroseHydrogelSurface modificationCell adhesion |
| spellingShingle | Xueying Yu Fang Cheng Wei He Microgels-on-macrogel: A simple cytophilic surface makeover of soft agarose substrates JCIS Open Microgel Colloid Agarose Hydrogel Surface modification Cell adhesion |
| title | Microgels-on-macrogel: A simple cytophilic surface makeover of soft agarose substrates |
| title_full | Microgels-on-macrogel: A simple cytophilic surface makeover of soft agarose substrates |
| title_fullStr | Microgels-on-macrogel: A simple cytophilic surface makeover of soft agarose substrates |
| title_full_unstemmed | Microgels-on-macrogel: A simple cytophilic surface makeover of soft agarose substrates |
| title_short | Microgels-on-macrogel: A simple cytophilic surface makeover of soft agarose substrates |
| title_sort | microgels on macrogel a simple cytophilic surface makeover of soft agarose substrates |
| topic | Microgel Colloid Agarose Hydrogel Surface modification Cell adhesion |
| url | http://www.sciencedirect.com/science/article/pii/S2666934X22000149 |
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