Fixation and Visualization of Full Protein Corona on Lipid Surface of Composite Nanoconstruction
Spontaneous sorption of proteins on the nanoparticles’ surface leads to the fact that nanoparticles in biological media are always enveloped by a layer of proteins—the protein corona. Corona proteins affect the properties of nanoparticles and their behavior in a biological environment. In this regar...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-12-01
|
| Series: | Nanomaterials |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-4991/13/24/3094 |
| _version_ | 1797379880715812864 |
|---|---|
| author | Anna V. Epanchintseva Julia E. Poletaeva Irina A. Bakhno Vladimir V. Belov Alina E. Grigor’eva Svetlana V. Baranova Elena I. Ryabchikova Ilya S. Dovydenko |
| author_facet | Anna V. Epanchintseva Julia E. Poletaeva Irina A. Bakhno Vladimir V. Belov Alina E. Grigor’eva Svetlana V. Baranova Elena I. Ryabchikova Ilya S. Dovydenko |
| author_sort | Anna V. Epanchintseva |
| collection | DOAJ |
| description | Spontaneous sorption of proteins on the nanoparticles’ surface leads to the fact that nanoparticles in biological media are always enveloped by a layer of proteins—the protein corona. Corona proteins affect the properties of nanoparticles and their behavior in a biological environment. In this regard, knowledge about the composition of the corona is a necessary element for the development of nanomedicine. Because proteins have different sorption efficacy, isolating particles with a full corona and characterizing the full corona is challenging. In this study, we propose a photo-activated cross-linker for full protein corona fixation. We believe that the application of our proposed approach will make it possible to capture and visualize the full corona on nanoparticles coated with a lipid shell. |
| first_indexed | 2024-03-08T20:29:27Z |
| format | Article |
| id | doaj.art-df5888643f5f4238a5d216d9496b728e |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-08T20:29:27Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-df5888643f5f4238a5d216d9496b728e2023-12-22T14:29:01ZengMDPI AGNanomaterials2079-49912023-12-011324309410.3390/nano13243094Fixation and Visualization of Full Protein Corona on Lipid Surface of Composite NanoconstructionAnna V. Epanchintseva0Julia E. Poletaeva1Irina A. Bakhno2Vladimir V. Belov3Alina E. Grigor’eva4Svetlana V. Baranova5Elena I. Ryabchikova6Ilya S. Dovydenko7Institute of Chemical Biology and Fundamental Medicine SB RAS, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine SB RAS, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine SB RAS, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine SB RAS, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine SB RAS, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine SB RAS, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine SB RAS, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine SB RAS, 630090 Novosibirsk, RussiaSpontaneous sorption of proteins on the nanoparticles’ surface leads to the fact that nanoparticles in biological media are always enveloped by a layer of proteins—the protein corona. Corona proteins affect the properties of nanoparticles and their behavior in a biological environment. In this regard, knowledge about the composition of the corona is a necessary element for the development of nanomedicine. Because proteins have different sorption efficacy, isolating particles with a full corona and characterizing the full corona is challenging. In this study, we propose a photo-activated cross-linker for full protein corona fixation. We believe that the application of our proposed approach will make it possible to capture and visualize the full corona on nanoparticles coated with a lipid shell.https://www.mdpi.com/2079-4991/13/24/3094protein coronamultilevel nanoconstructionsserum proteinsphotomodificationfixation of full coronafull corona visualization |
| spellingShingle | Anna V. Epanchintseva Julia E. Poletaeva Irina A. Bakhno Vladimir V. Belov Alina E. Grigor’eva Svetlana V. Baranova Elena I. Ryabchikova Ilya S. Dovydenko Fixation and Visualization of Full Protein Corona on Lipid Surface of Composite Nanoconstruction Nanomaterials protein corona multilevel nanoconstructions serum proteins photomodification fixation of full corona full corona visualization |
| title | Fixation and Visualization of Full Protein Corona on Lipid Surface of Composite Nanoconstruction |
| title_full | Fixation and Visualization of Full Protein Corona on Lipid Surface of Composite Nanoconstruction |
| title_fullStr | Fixation and Visualization of Full Protein Corona on Lipid Surface of Composite Nanoconstruction |
| title_full_unstemmed | Fixation and Visualization of Full Protein Corona on Lipid Surface of Composite Nanoconstruction |
| title_short | Fixation and Visualization of Full Protein Corona on Lipid Surface of Composite Nanoconstruction |
| title_sort | fixation and visualization of full protein corona on lipid surface of composite nanoconstruction |
| topic | protein corona multilevel nanoconstructions serum proteins photomodification fixation of full corona full corona visualization |
| url | https://www.mdpi.com/2079-4991/13/24/3094 |
| work_keys_str_mv | AT annavepanchintseva fixationandvisualizationoffullproteincoronaonlipidsurfaceofcompositenanoconstruction AT juliaepoletaeva fixationandvisualizationoffullproteincoronaonlipidsurfaceofcompositenanoconstruction AT irinaabakhno fixationandvisualizationoffullproteincoronaonlipidsurfaceofcompositenanoconstruction AT vladimirvbelov fixationandvisualizationoffullproteincoronaonlipidsurfaceofcompositenanoconstruction AT alinaegrigoreva fixationandvisualizationoffullproteincoronaonlipidsurfaceofcompositenanoconstruction AT svetlanavbaranova fixationandvisualizationoffullproteincoronaonlipidsurfaceofcompositenanoconstruction AT elenairyabchikova fixationandvisualizationoffullproteincoronaonlipidsurfaceofcompositenanoconstruction AT ilyasdovydenko fixationandvisualizationoffullproteincoronaonlipidsurfaceofcompositenanoconstruction |