Molecular Study of Selected Taxonomically Critical Taxa of the Genus <i>Iris</i> L. from the Broader Alpine-Dinaric Area
Some wild, morphologically diverse taxa of the genus <i>Iris</i> in the broad Alpine-Dinaric area have never been explored molecularly, and/or have ambiguous systematic status. The main aims of our research were to perform a molecular study of critical <i>Iris</i> taxa from t...
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MDPI AG
2020-09-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/9/9/1229 |
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| author | Tim Weber Jernej Jakše Barbara Sladonja Dario Hruševar Nediljko Landeka Slavko Brana Borut Bohanec Milenko Milović Dalibor Vladović Božena Mitić Danijela Poljuha |
| author_facet | Tim Weber Jernej Jakše Barbara Sladonja Dario Hruševar Nediljko Landeka Slavko Brana Borut Bohanec Milenko Milović Dalibor Vladović Božena Mitić Danijela Poljuha |
| author_sort | Tim Weber |
| collection | DOAJ |
| description | Some wild, morphologically diverse taxa of the genus <i>Iris</i> in the broad Alpine-Dinaric area have never been explored molecularly, and/or have ambiguous systematic status. The main aims of our research were to perform a molecular study of critical <i>Iris</i> taxa from that area (especially a narrow endemic accepted species <i>I. adriatica</i>, for which we also analysed genome size) and to explore the contribution of eight microsatellites and highly variable chloroplast DNA (<i>ndhJ</i>, <i>rpoC1</i>) markers to the understanding of the <i>Iris</i> taxa taxonomy and phylogeny. Both the microsatellite-based UPGMA and plastid markers-based maximum likelihood analysis discriminated three main clusters in the set of 32 analysed samples, which correspond well to the lower taxonomic categories of the genus, and support separate status of ambiguous regional taxa (e.g., <i>I. sibirica</i> subsp. <i>erirrhiza</i>, <i>I.</i> x <i>croatica</i> and <i>I.</i> x <i>rotschildii</i>). The first molecular data on <i>I. adriatica</i> revealed its genome size (2C = 12.639 ± 0.202 pg) and indicated the existence of ecotypes. For future molecular characterisation of the genus we recommend the utilisation of microsatellite markers supplemented with a combination of plastid markers. |
| first_indexed | 2024-03-10T16:15:00Z |
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| language | English |
| last_indexed | 2024-03-10T16:15:00Z |
| publishDate | 2020-09-01 |
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| series | Plants |
| spelling | doaj.art-df59726d4a3d44ebbdd8e55a278c0ee72023-11-20T14:11:12ZengMDPI AGPlants2223-77472020-09-0199122910.3390/plants9091229Molecular Study of Selected Taxonomically Critical Taxa of the Genus <i>Iris</i> L. from the Broader Alpine-Dinaric AreaTim Weber0Jernej Jakše1Barbara Sladonja2Dario Hruševar3Nediljko Landeka4Slavko Brana5Borut Bohanec6Milenko Milović7Dalibor Vladović8Božena Mitić9Danijela Poljuha10Institute of Agriculture and Tourism, Karla Huguesa 8, HR-52440 Poreč, CroatiaBiotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, SI-1000 Ljubljana, SloveniaInstitute of Agriculture and Tourism, Karla Huguesa 8, HR-52440 Poreč, CroatiaDepartment of Biology, Faculty of Science, University of Zagreb, Rooseveltov trg 6, HR-10000 Zagreb, CroatiaPublic Health Institute of the Istrian Region, Nazorova 23, HR-52100 Pula, CroatiaPublic Institution Natura Histrica, Riva 8, HR-52100 Pula, CroatiaBiotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, SI-1000 Ljubljana, SloveniaMedical and Chemical School, Ante Šupuk Street 29, HR-22000 Šibenik, CroatiaNature History Museum Split, Poljana kneza Trpimira 3, HR-21000 Split, CroatiaDepartment of Biology, Faculty of Science, University of Zagreb, Rooseveltov trg 6, HR-10000 Zagreb, CroatiaInstitute of Agriculture and Tourism, Karla Huguesa 8, HR-52440 Poreč, CroatiaSome wild, morphologically diverse taxa of the genus <i>Iris</i> in the broad Alpine-Dinaric area have never been explored molecularly, and/or have ambiguous systematic status. The main aims of our research were to perform a molecular study of critical <i>Iris</i> taxa from that area (especially a narrow endemic accepted species <i>I. adriatica</i>, for which we also analysed genome size) and to explore the contribution of eight microsatellites and highly variable chloroplast DNA (<i>ndhJ</i>, <i>rpoC1</i>) markers to the understanding of the <i>Iris</i> taxa taxonomy and phylogeny. Both the microsatellite-based UPGMA and plastid markers-based maximum likelihood analysis discriminated three main clusters in the set of 32 analysed samples, which correspond well to the lower taxonomic categories of the genus, and support separate status of ambiguous regional taxa (e.g., <i>I. sibirica</i> subsp. <i>erirrhiza</i>, <i>I.</i> x <i>croatica</i> and <i>I.</i> x <i>rotschildii</i>). The first molecular data on <i>I. adriatica</i> revealed its genome size (2C = 12.639 ± 0.202 pg) and indicated the existence of ecotypes. For future molecular characterisation of the genus we recommend the utilisation of microsatellite markers supplemented with a combination of plastid markers.https://www.mdpi.com/2223-7747/9/9/1229IridaceaeEuropechloroplast DNAmicrosatellitesphylogenytaxonomy |
| spellingShingle | Tim Weber Jernej Jakše Barbara Sladonja Dario Hruševar Nediljko Landeka Slavko Brana Borut Bohanec Milenko Milović Dalibor Vladović Božena Mitić Danijela Poljuha Molecular Study of Selected Taxonomically Critical Taxa of the Genus <i>Iris</i> L. from the Broader Alpine-Dinaric Area Plants Iridaceae Europe chloroplast DNA microsatellites phylogeny taxonomy |
| title | Molecular Study of Selected Taxonomically Critical Taxa of the Genus <i>Iris</i> L. from the Broader Alpine-Dinaric Area |
| title_full | Molecular Study of Selected Taxonomically Critical Taxa of the Genus <i>Iris</i> L. from the Broader Alpine-Dinaric Area |
| title_fullStr | Molecular Study of Selected Taxonomically Critical Taxa of the Genus <i>Iris</i> L. from the Broader Alpine-Dinaric Area |
| title_full_unstemmed | Molecular Study of Selected Taxonomically Critical Taxa of the Genus <i>Iris</i> L. from the Broader Alpine-Dinaric Area |
| title_short | Molecular Study of Selected Taxonomically Critical Taxa of the Genus <i>Iris</i> L. from the Broader Alpine-Dinaric Area |
| title_sort | molecular study of selected taxonomically critical taxa of the genus i iris i l from the broader alpine dinaric area |
| topic | Iridaceae Europe chloroplast DNA microsatellites phylogeny taxonomy |
| url | https://www.mdpi.com/2223-7747/9/9/1229 |
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