Dietary methionine source alters the lipidome in the small intestinal epithelium of pigs

Abstract Methionine (Met) as an essential amino acid has key importance in a variety of metabolic pathways. This study investigated the influence of three dietary Met supplements (0.21% L-Met, 0.21% DL-Met and 0.31% DL-2-hydroxy-4-(methylthio)butanoic acid (DL-HMTBA)) on the metabolome and inflammat...

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Main Authors: Isabel I. Schermuly, Stella Romanet, Martina Klünemann, Lucia Mastrototaro, Robert Pieper, Jürgen Zentek, Rose A. Whelan, Jörg R. Aschenbach
Format: Article
Language:English
Published: Nature Portfolio 2022-03-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-022-08933-3
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author Isabel I. Schermuly
Stella Romanet
Martina Klünemann
Lucia Mastrototaro
Robert Pieper
Jürgen Zentek
Rose A. Whelan
Jörg R. Aschenbach
author_facet Isabel I. Schermuly
Stella Romanet
Martina Klünemann
Lucia Mastrototaro
Robert Pieper
Jürgen Zentek
Rose A. Whelan
Jörg R. Aschenbach
author_sort Isabel I. Schermuly
collection DOAJ
description Abstract Methionine (Met) as an essential amino acid has key importance in a variety of metabolic pathways. This study investigated the influence of three dietary Met supplements (0.21% L-Met, 0.21% DL-Met and 0.31% DL-2-hydroxy-4-(methylthio)butanoic acid (DL-HMTBA)) on the metabolome and inflammatory status in the small intestine of pigs. Epithelia from duodenum, proximal jejunum, middle jejunum and ileum were subjected to metabolomics analysis and qRT-PCR of caspase 1, NLR family pyrin domain containing 3 (NLRP3), interleukins IL1β, IL8, IL18, and transforming growth factor TGFβ. Principal component analysis of the intraepithelial metabolome revealed strong clustering of samples by intestinal segment but not by dietary treatment. However, pathway enrichment analysis revealed that after L-Met supplementation polyunsaturated fatty acids (PUFA) and tocopherol metabolites were lower across small intestinal segments, whereas monohydroxy fatty acids were increased in distal small intestine. Pigs supplemented with DL-HMTBA showed a pronounced shift of secondary bile acids (BA) and sphingosine metabolites from middle jejunum to ileum. In the amino acid super pathway, only histidine metabolism tended to be altered in DL-Met-supplemented pigs. Diet did not affect the expression of inflammation-related genes. These findings suggest that dietary supplementation of young pigs with different Met sources selectively alters lipid metabolism without consequences for inflammatory status.
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spelling doaj.art-df59f76c42364713912e49efb77c7a622022-12-21T21:11:26ZengNature PortfolioScientific Reports2045-23222022-03-0112111410.1038/s41598-022-08933-3Dietary methionine source alters the lipidome in the small intestinal epithelium of pigsIsabel I. Schermuly0Stella Romanet1Martina Klünemann2Lucia Mastrototaro3Robert Pieper4Jürgen Zentek5Rose A. Whelan6Jörg R. Aschenbach7Institute of Veterinary Physiology, Freie Universität BerlinInstitute of Veterinary Physiology, Freie Universität BerlinEvonik Operations GmbH, Animal Nutrition ServicesInstitute of Veterinary Physiology, Freie Universität BerlinInstitute of Animal Nutrition, Freie Universität BerlinInstitute of Animal Nutrition, Freie Universität BerlinEvonik Operations GmbH, Animal Nutrition ServicesInstitute of Veterinary Physiology, Freie Universität BerlinAbstract Methionine (Met) as an essential amino acid has key importance in a variety of metabolic pathways. This study investigated the influence of three dietary Met supplements (0.21% L-Met, 0.21% DL-Met and 0.31% DL-2-hydroxy-4-(methylthio)butanoic acid (DL-HMTBA)) on the metabolome and inflammatory status in the small intestine of pigs. Epithelia from duodenum, proximal jejunum, middle jejunum and ileum were subjected to metabolomics analysis and qRT-PCR of caspase 1, NLR family pyrin domain containing 3 (NLRP3), interleukins IL1β, IL8, IL18, and transforming growth factor TGFβ. Principal component analysis of the intraepithelial metabolome revealed strong clustering of samples by intestinal segment but not by dietary treatment. However, pathway enrichment analysis revealed that after L-Met supplementation polyunsaturated fatty acids (PUFA) and tocopherol metabolites were lower across small intestinal segments, whereas monohydroxy fatty acids were increased in distal small intestine. Pigs supplemented with DL-HMTBA showed a pronounced shift of secondary bile acids (BA) and sphingosine metabolites from middle jejunum to ileum. In the amino acid super pathway, only histidine metabolism tended to be altered in DL-Met-supplemented pigs. Diet did not affect the expression of inflammation-related genes. These findings suggest that dietary supplementation of young pigs with different Met sources selectively alters lipid metabolism without consequences for inflammatory status.https://doi.org/10.1038/s41598-022-08933-3
spellingShingle Isabel I. Schermuly
Stella Romanet
Martina Klünemann
Lucia Mastrototaro
Robert Pieper
Jürgen Zentek
Rose A. Whelan
Jörg R. Aschenbach
Dietary methionine source alters the lipidome in the small intestinal epithelium of pigs
Scientific Reports
title Dietary methionine source alters the lipidome in the small intestinal epithelium of pigs
title_full Dietary methionine source alters the lipidome in the small intestinal epithelium of pigs
title_fullStr Dietary methionine source alters the lipidome in the small intestinal epithelium of pigs
title_full_unstemmed Dietary methionine source alters the lipidome in the small intestinal epithelium of pigs
title_short Dietary methionine source alters the lipidome in the small intestinal epithelium of pigs
title_sort dietary methionine source alters the lipidome in the small intestinal epithelium of pigs
url https://doi.org/10.1038/s41598-022-08933-3
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