A metabolomic strategy defines the regulation of lipid content and global metabolism by Δ9 desaturases in <it>Caenorhabditis elegans</it>
<p>Abstract</p> <p>Background</p> <p><it>Caenorhabditis elegans </it>provides a genetically tractable model organism to investigate the network of genes involved in fat metabolism and how regulation is perturbed to produce the complex phenotype of obesity. &...
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BMC
2012-01-01
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| Series: | BMC Genomics |
| Online Access: | http://www.biomedcentral.com/1471-2164/13/36 |
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| author | Castro Cecilia Sar Funda Shaw W Robert Mishima Masanori Miska Eric A Griffin Julian L |
| author_facet | Castro Cecilia Sar Funda Shaw W Robert Mishima Masanori Miska Eric A Griffin Julian L |
| author_sort | Castro Cecilia |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p><it>Caenorhabditis elegans </it>provides a genetically tractable model organism to investigate the network of genes involved in fat metabolism and how regulation is perturbed to produce the complex phenotype of obesity. <it>C. elegans </it>possess the full range of desaturases, including the Δ9 desaturases expressed by <it>fat-5, fat-6 </it>and <it>fat-7</it>. They regulate the biosynthesis of monounsaturated fatty acids, used for the synthesis of lipids including phospholipids, triglycerides and cholesteryl esters.</p> <p>Results</p> <p>Liquid chromatography mass spectrometry (LC-MS), gas chromatography mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR) spectroscopy were used to define the metabolome of all the possible knock-outs for the Δ9 desaturases, including for the first time intact lipids. Despite the genes having similar enzymatic roles, excellent discrimination was achievable for all single and viable double mutants highlighting the distinctive roles of <it>fat-6 </it>and <it>fat-7</it>, both expressing steroyl-CoA desaturases. The metabolomic changes extend to aqueous metabolites demonstrating the influence Δ9 desaturases have on regulating global metabolism and highlighting how comprehensive metabolomics is more discriminatory than classically used dyes for fat staining.</p> <p>Conclusions</p> <p>The propagation of metabolic changes across the network of metabolism demonstrates that modification of the Δ9 desaturases places C.elegans into a catabolic state compared with wildtype controls.</p> |
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| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-17T12:01:23Z |
| publishDate | 2012-01-01 |
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| series | BMC Genomics |
| spelling | doaj.art-df5df697ff38477e8c1f1890f51f23652022-12-21T21:49:50ZengBMCBMC Genomics1471-21642012-01-011313610.1186/1471-2164-13-36A metabolomic strategy defines the regulation of lipid content and global metabolism by Δ9 desaturases in <it>Caenorhabditis elegans</it>Castro CeciliaSar FundaShaw W RobertMishima MasanoriMiska Eric AGriffin Julian L<p>Abstract</p> <p>Background</p> <p><it>Caenorhabditis elegans </it>provides a genetically tractable model organism to investigate the network of genes involved in fat metabolism and how regulation is perturbed to produce the complex phenotype of obesity. <it>C. elegans </it>possess the full range of desaturases, including the Δ9 desaturases expressed by <it>fat-5, fat-6 </it>and <it>fat-7</it>. They regulate the biosynthesis of monounsaturated fatty acids, used for the synthesis of lipids including phospholipids, triglycerides and cholesteryl esters.</p> <p>Results</p> <p>Liquid chromatography mass spectrometry (LC-MS), gas chromatography mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR) spectroscopy were used to define the metabolome of all the possible knock-outs for the Δ9 desaturases, including for the first time intact lipids. Despite the genes having similar enzymatic roles, excellent discrimination was achievable for all single and viable double mutants highlighting the distinctive roles of <it>fat-6 </it>and <it>fat-7</it>, both expressing steroyl-CoA desaturases. The metabolomic changes extend to aqueous metabolites demonstrating the influence Δ9 desaturases have on regulating global metabolism and highlighting how comprehensive metabolomics is more discriminatory than classically used dyes for fat staining.</p> <p>Conclusions</p> <p>The propagation of metabolic changes across the network of metabolism demonstrates that modification of the Δ9 desaturases places C.elegans into a catabolic state compared with wildtype controls.</p>http://www.biomedcentral.com/1471-2164/13/36 |
| spellingShingle | Castro Cecilia Sar Funda Shaw W Robert Mishima Masanori Miska Eric A Griffin Julian L A metabolomic strategy defines the regulation of lipid content and global metabolism by Δ9 desaturases in <it>Caenorhabditis elegans</it> BMC Genomics |
| title | A metabolomic strategy defines the regulation of lipid content and global metabolism by Δ9 desaturases in <it>Caenorhabditis elegans</it> |
| title_full | A metabolomic strategy defines the regulation of lipid content and global metabolism by Δ9 desaturases in <it>Caenorhabditis elegans</it> |
| title_fullStr | A metabolomic strategy defines the regulation of lipid content and global metabolism by Δ9 desaturases in <it>Caenorhabditis elegans</it> |
| title_full_unstemmed | A metabolomic strategy defines the regulation of lipid content and global metabolism by Δ9 desaturases in <it>Caenorhabditis elegans</it> |
| title_short | A metabolomic strategy defines the regulation of lipid content and global metabolism by Δ9 desaturases in <it>Caenorhabditis elegans</it> |
| title_sort | metabolomic strategy defines the regulation of lipid content and global metabolism by δ9 desaturases in it caenorhabditis elegans it |
| url | http://www.biomedcentral.com/1471-2164/13/36 |
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