Purification and characterization of a novel thermoacidophilic and thermostable α-mannosidase from the digestive fluid of oil palm weevil Rhynchophorus palmarum (Coleoptera: Curculionidae) larvae
An extracellular α-mannosidase with unusual properties was purified from the digestive fluid of oil palm weevil (Rhynchophorus palmarum Linnaeus) larvae using ammonium sulphate saturation, size exclusion and anion-exchange chromatography. The enzyme named RpltM is thermoacidophilic, thermostable and...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Institute of Entomology, Biology Centre, Czech Academy of Science
2009-05-01
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| Series: | European Journal of Entomology |
| Subjects: | |
| Online Access: | https://www.eje.cz/artkey/eje-200902-0005_Purification_and_characterization_of_a_novel_thermoacidophilic_and_thermostable_alpha_-mannosidase_from_the_di.php |
| Summary: | An extracellular α-mannosidase with unusual properties was purified from the digestive fluid of oil palm weevil (Rhynchophorus palmarum Linnaeus) larvae using ammonium sulphate saturation, size exclusion and anion-exchange chromatography. The enzyme named RpltM is thermoacidophilic, thermostable and behaves like lysosomal α-mannosidase (EC 3.2.1.24). The molecular weight, Km value, optimum reaction temperature and pH are 108-112 kDa, 0.36 mM, 65°C and 4.5, respectively. Zn2+ enhanced whereas Cu2+, Sodium dodecyl sulphate, swainsonine and 1,4-dideoxy-1,4-iminomannitol strongly inhibited its hydrolytic activity. The enzyme was stable for 25 min at 65°C and retained 70% of its initial activity after 60 min. At 70°C, around 60% of this activity was conserved after 25 min. RpltM retained more than 90% of its activity over a pH range of 4.2 to 5.0 and remained fully active in the presence of detergents such as nonidet P-40, triton X-100, polyoxyethylen-10-oleyl ether (up to 1%, w/v), dithiothreitol and β-mercaptoethanol. The stability under these conditions is also better than that reported for other insect α-mannosidases. Thus, RpltM could be used as an important bioindustrial tool for removing mannose residues from oligosaccharides. |
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| ISSN: | 1210-5759 1802-8829 |