The Impact of Different Cultivation Systems on the Content of Selected Secondary Metabolites and Antioxidant Activity of <i>Carlina acaulis</i> Plant Material
Roots and leaves of <i>Carlina acaulis</i> L. are still used in ethnomedicine in many European countries; however, the limited occurrence of the plants and protection of this species necessitate a search for alternative ways for obtaining this plant material. In this study, in vitro cult...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-12-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/1/146 |
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| author | Maciej Strzemski Sławomir Dresler Ireneusz Sowa Anna Czubacka Monika Agacka-Mołdoch Bartosz J. Płachno Sebastian Granica Marcin Feldo Magdalena Wójciak-Kosior |
| author_facet | Maciej Strzemski Sławomir Dresler Ireneusz Sowa Anna Czubacka Monika Agacka-Mołdoch Bartosz J. Płachno Sebastian Granica Marcin Feldo Magdalena Wójciak-Kosior |
| author_sort | Maciej Strzemski |
| collection | DOAJ |
| description | Roots and leaves of <i>Carlina acaulis</i> L. are still used in ethnomedicine in many European countries; however, the limited occurrence of the plants and protection of this species necessitate a search for alternative ways for obtaining this plant material. In this study, in vitro cultures, hydroponic cultures, and field cultivation were applied to obtain the <i>C. acaulis</i> plant material. Its quality was evaluated using antioxidant activity tests and high performance liquid chromatography analysis. Our study showed that the antioxidant activity and the content of chlorogenic and 3,5-di-caffeoylquinic acid in roots of plants cultivated in hydroponics and field conditions were comparable. However, the amount of carlina oxide was significantly higher in plants from the field. The flavonoid content in leaves obtained from both cultivation systems was at the same level; however, the antioxidant activity and the content of the investigated metabolites were higher in the soil cultivation system. The callus line exhibited high differentiation in phytochemical compositions depending on the treatments and medium compositions. |
| first_indexed | 2024-12-22T05:28:36Z |
| format | Article |
| id | doaj.art-df628917ab554c3d9ae81e67b0f127b8 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-22T05:28:36Z |
| publishDate | 2019-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-df628917ab554c3d9ae81e67b0f127b82022-12-21T18:37:30ZengMDPI AGMolecules1420-30492019-12-0125114610.3390/molecules25010146molecules25010146The Impact of Different Cultivation Systems on the Content of Selected Secondary Metabolites and Antioxidant Activity of <i>Carlina acaulis</i> Plant MaterialMaciej Strzemski0Sławomir Dresler1Ireneusz Sowa2Anna Czubacka3Monika Agacka-Mołdoch4Bartosz J. Płachno5Sebastian Granica6Marcin Feldo7Magdalena Wójciak-Kosior8Department of Analytical Chemistry, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, PolandDepartment of Plant Physiology and Biophysics, Institute of Biological Science, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, PolandDepartment of Analytical Chemistry, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, PolandDepartment of Plant Breeding and Biotechnology, Institute of Soil Science and Plant Cultivation, State Research Institute, Czartoryskich 8 St., 24-100 Puławy, PolandDepartment of Plant Breeding and Biotechnology, Institute of Soil Science and Plant Cultivation, State Research Institute, Czartoryskich 8 St., 24-100 Puławy, PolandDepartment of Plant Cytology and Embryology, Institute of Botany, Faculty of Biology, Jagiellonian University, Gronostajowa 9 St. 30-387 Cracow, PolandDepartment of Pharmacognosy and Molecular Basis of Phytotherapy, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1 St., 02-097 Warsaw, PolandDepartment of Vascular Surgery, Medical University of Lublin, Staszica 11 St., 20-081 Lublin, PolandDepartment of Analytical Chemistry, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, PolandRoots and leaves of <i>Carlina acaulis</i> L. are still used in ethnomedicine in many European countries; however, the limited occurrence of the plants and protection of this species necessitate a search for alternative ways for obtaining this plant material. In this study, in vitro cultures, hydroponic cultures, and field cultivation were applied to obtain the <i>C. acaulis</i> plant material. Its quality was evaluated using antioxidant activity tests and high performance liquid chromatography analysis. Our study showed that the antioxidant activity and the content of chlorogenic and 3,5-di-caffeoylquinic acid in roots of plants cultivated in hydroponics and field conditions were comparable. However, the amount of carlina oxide was significantly higher in plants from the field. The flavonoid content in leaves obtained from both cultivation systems was at the same level; however, the antioxidant activity and the content of the investigated metabolites were higher in the soil cultivation system. The callus line exhibited high differentiation in phytochemical compositions depending on the treatments and medium compositions.https://www.mdpi.com/1420-3049/25/1/146<i>carlina acaulis</i>callus tissuedepsidespentacyclic triterpenesantioxidant activityhydroponicsoil cultivation |
| spellingShingle | Maciej Strzemski Sławomir Dresler Ireneusz Sowa Anna Czubacka Monika Agacka-Mołdoch Bartosz J. Płachno Sebastian Granica Marcin Feldo Magdalena Wójciak-Kosior The Impact of Different Cultivation Systems on the Content of Selected Secondary Metabolites and Antioxidant Activity of <i>Carlina acaulis</i> Plant Material Molecules <i>carlina acaulis</i> callus tissue depsides pentacyclic triterpenes antioxidant activity hydroponic soil cultivation |
| title | The Impact of Different Cultivation Systems on the Content of Selected Secondary Metabolites and Antioxidant Activity of <i>Carlina acaulis</i> Plant Material |
| title_full | The Impact of Different Cultivation Systems on the Content of Selected Secondary Metabolites and Antioxidant Activity of <i>Carlina acaulis</i> Plant Material |
| title_fullStr | The Impact of Different Cultivation Systems on the Content of Selected Secondary Metabolites and Antioxidant Activity of <i>Carlina acaulis</i> Plant Material |
| title_full_unstemmed | The Impact of Different Cultivation Systems on the Content of Selected Secondary Metabolites and Antioxidant Activity of <i>Carlina acaulis</i> Plant Material |
| title_short | The Impact of Different Cultivation Systems on the Content of Selected Secondary Metabolites and Antioxidant Activity of <i>Carlina acaulis</i> Plant Material |
| title_sort | impact of different cultivation systems on the content of selected secondary metabolites and antioxidant activity of i carlina acaulis i plant material |
| topic | <i>carlina acaulis</i> callus tissue depsides pentacyclic triterpenes antioxidant activity hydroponic soil cultivation |
| url | https://www.mdpi.com/1420-3049/25/1/146 |
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