Alteration of the Fatty Acid Metabolism in the Rat Kidney Caused by the Injection of Serum from Patients with Collapsing Glomerulopathy
Patients with collapsing glomerulopathy (CG) have marked proteinuria that rapidly progresses to chronic renal failure. In this study, we investigated if the nephropathy produced in a rat model by the injection of serum from CG patients induced alterations in fatty acid (FA) metabolism. Twenty-four f...
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MDPI AG
2020-09-01
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| Online Access: | https://www.mdpi.com/2227-9059/8/10/388 |
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| author | Elizabeth Soria-Castro Verónica Guarner-Lans María Elena Soto María del Carmen Avila-Casado Linaloe Manzano Pech Israel Pérez-Torres |
| author_facet | Elizabeth Soria-Castro Verónica Guarner-Lans María Elena Soto María del Carmen Avila-Casado Linaloe Manzano Pech Israel Pérez-Torres |
| author_sort | Elizabeth Soria-Castro |
| collection | DOAJ |
| description | Patients with collapsing glomerulopathy (CG) have marked proteinuria that rapidly progresses to chronic renal failure. In this study, we investigated if the nephropathy produced in a rat model by the injection of serum from CG patients induced alterations in fatty acid (FA) metabolism. Twenty-four female Sprague-Dawley rats were divided into four groups of six rats each: Group I, control rats (C); Group II, rats that received injections of 1 mL of 0.9% NaCl saline solution (SS); Group III, rats injected with 25 mg/mL of serum from healthy subjects (HS); and Group IV, rats injected with 25 mg/mL of serum from CG patients. In all groups, the systolic blood pressure (SBP), proteinuria, creatinine clearance (CC), cholesterol and total FA composition in the kidney and serum were evaluated. The administration of serum from CG patients to rats induced glomerular collapse, proteinuria, reduced CC and elevated SBP (<i>p</i> ≤ 0.01) in comparison with the C, SS and HS rats. The FA composition of the serum of rats that received the CG serum showed an increase in palmitic acid (PA) and a decrease in arachidonic acid (AA) when compared to serum from HS (<i>p</i> ≤ 0.02). In rats receiving the CG serum, there was also a decrease in the AA in the kidney but there was an increase in the PA in the serum and kidney (<i>p</i> ≤ 0.01). These results suggest that the administration of serum from CG patients to rats induces alterations in FA metabolism including changes in PA and in AA, which are precursors for the biosynthesis of the prostaglandins that are involved in the elevation of SBP and in renal injury. These changes may contribute to collapsing glomerulopathy disease. |
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| spelling | doaj.art-df62a22f594448fb8f3eb83fe82c21ac2023-11-20T15:28:50ZengMDPI AGBiomedicines2227-90592020-09-0181038810.3390/biomedicines8100388Alteration of the Fatty Acid Metabolism in the Rat Kidney Caused by the Injection of Serum from Patients with Collapsing GlomerulopathyElizabeth Soria-Castro0Verónica Guarner-Lans1María Elena Soto2María del Carmen Avila-Casado3Linaloe Manzano Pech4Israel Pérez-Torres5Cardiovascular Biomedicine, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano 1, Col. Sección XVI, Mexico City 14080, MexicoPhysiology Department, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano 1, Sección XVI, Tlalpan, Mexico City 14080, MexicoImmunology Department, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano 1, Sección XVI, Tlalpan, Mexico City 14080, MexicoPathology Department, University Health Network, Toronto General Hospital, 200 Elizabeth Street, Toronto, ON M5G 2C4, CanadaCardiovascular Biomedicine, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano 1, Col. Sección XVI, Mexico City 14080, MexicoCardiovascular Biomedicine, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano 1, Col. Sección XVI, Mexico City 14080, MexicoPatients with collapsing glomerulopathy (CG) have marked proteinuria that rapidly progresses to chronic renal failure. In this study, we investigated if the nephropathy produced in a rat model by the injection of serum from CG patients induced alterations in fatty acid (FA) metabolism. Twenty-four female Sprague-Dawley rats were divided into four groups of six rats each: Group I, control rats (C); Group II, rats that received injections of 1 mL of 0.9% NaCl saline solution (SS); Group III, rats injected with 25 mg/mL of serum from healthy subjects (HS); and Group IV, rats injected with 25 mg/mL of serum from CG patients. In all groups, the systolic blood pressure (SBP), proteinuria, creatinine clearance (CC), cholesterol and total FA composition in the kidney and serum were evaluated. The administration of serum from CG patients to rats induced glomerular collapse, proteinuria, reduced CC and elevated SBP (<i>p</i> ≤ 0.01) in comparison with the C, SS and HS rats. The FA composition of the serum of rats that received the CG serum showed an increase in palmitic acid (PA) and a decrease in arachidonic acid (AA) when compared to serum from HS (<i>p</i> ≤ 0.02). In rats receiving the CG serum, there was also a decrease in the AA in the kidney but there was an increase in the PA in the serum and kidney (<i>p</i> ≤ 0.01). These results suggest that the administration of serum from CG patients to rats induces alterations in FA metabolism including changes in PA and in AA, which are precursors for the biosynthesis of the prostaglandins that are involved in the elevation of SBP and in renal injury. These changes may contribute to collapsing glomerulopathy disease.https://www.mdpi.com/2227-9059/8/10/388collapsing glomerulopathysystolic blood pressurefatty acidsproteinuria |
| spellingShingle | Elizabeth Soria-Castro Verónica Guarner-Lans María Elena Soto María del Carmen Avila-Casado Linaloe Manzano Pech Israel Pérez-Torres Alteration of the Fatty Acid Metabolism in the Rat Kidney Caused by the Injection of Serum from Patients with Collapsing Glomerulopathy Biomedicines collapsing glomerulopathy systolic blood pressure fatty acids proteinuria |
| title | Alteration of the Fatty Acid Metabolism in the Rat Kidney Caused by the Injection of Serum from Patients with Collapsing Glomerulopathy |
| title_full | Alteration of the Fatty Acid Metabolism in the Rat Kidney Caused by the Injection of Serum from Patients with Collapsing Glomerulopathy |
| title_fullStr | Alteration of the Fatty Acid Metabolism in the Rat Kidney Caused by the Injection of Serum from Patients with Collapsing Glomerulopathy |
| title_full_unstemmed | Alteration of the Fatty Acid Metabolism in the Rat Kidney Caused by the Injection of Serum from Patients with Collapsing Glomerulopathy |
| title_short | Alteration of the Fatty Acid Metabolism in the Rat Kidney Caused by the Injection of Serum from Patients with Collapsing Glomerulopathy |
| title_sort | alteration of the fatty acid metabolism in the rat kidney caused by the injection of serum from patients with collapsing glomerulopathy |
| topic | collapsing glomerulopathy systolic blood pressure fatty acids proteinuria |
| url | https://www.mdpi.com/2227-9059/8/10/388 |
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