Impact of Molasses on Ruminal Volatile Fatty Acid Production and Microbiota Composition In Vitro
The aim of this study was to assess if molasses could modify VFA production and the rumen microbial community in vitro. Three beet (treatment Beet) and three cane (treatment Cane) molasses preparations were randomly selected from a variety of samples collected worldwide and incubated in vitro with r...
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| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/4/728 |
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| author | A. Palmonari A. Federiconi D. Cavallini C. J. Sniffen L. Mammi S. Turroni F. D’Amico P. Holder A. Formigoni |
| author_facet | A. Palmonari A. Federiconi D. Cavallini C. J. Sniffen L. Mammi S. Turroni F. D’Amico P. Holder A. Formigoni |
| author_sort | A. Palmonari |
| collection | DOAJ |
| description | The aim of this study was to assess if molasses could modify VFA production and the rumen microbial community in vitro. Three beet (treatment Beet) and three cane (treatment Cane) molasses preparations were randomly selected from a variety of samples collected worldwide and incubated in vitro with rumen fluid along with a control sample (treatment CTR, in which no molasses was used). Flasks for VFA analysis were sampled at 0, 1, 2, 3, 4, 6, 8, and 24 h of each incubation. For microbiota analysis, samples from each fermentation flask after 12 and 24 h were subjected to microbial DNA extraction and V3–V4 16S rRNA gene sequencing on an Illumina MiSeq platform. Total net VFA production was higher in the beet and cane preparations than in the control (CTR) group at 24 h (33 mmol/L, 34 mmol/L, and 24.8 mmol/L, respectively), and the composition of VFAs was affected by the inclusion of molasses: acetic acid increased in the CTR group (73.5 mol%), while propionic acid increased in the beet and cane molasses (19.6 mol% and 18.6 mol%, respectively), and butyric acid increased, especially in the cane group (23.2 mol%). Molasses even influenced the composition of the rumen microbiota, and particularly the relative abundance of the most dominant family in the rumen, Prevotellaceae, which decreased compared to CTR (37.13%, 28.88%, and 49.6%, respectively). In contrast, Streptococcaceae (19.62% and 28.10% in molasses compared to 6.23% in CTR), Veillonellaceae (6.48% and 8.67% in molasses compared to 4.54% in CTR), and Fibrobacteraceae (0.90% and 0.88% in molasses compared to 0.62% in CTR) increased in the beet and cane groups compared to the CTR group. Another important finding is the lower proportion of Methanobacteriaceae following the addition of molasses compared to CTR (0.26%, 0.28%, and 0.43%, respectively). This study showed the impact of molasses in influencing VFA production and composition as a result of a modified rumen microbial composition. |
| first_indexed | 2024-03-11T09:16:25Z |
| format | Article |
| id | doaj.art-df67832da95d49089b30d7652132dc70 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T09:16:25Z |
| publishDate | 2023-02-01 |
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| record_format | Article |
| series | Animals |
| spelling | doaj.art-df67832da95d49089b30d7652132dc702023-11-16T18:40:45ZengMDPI AGAnimals2076-26152023-02-0113472810.3390/ani13040728Impact of Molasses on Ruminal Volatile Fatty Acid Production and Microbiota Composition In VitroA. Palmonari0A. Federiconi1D. Cavallini2C. J. Sniffen3L. Mammi4S. Turroni5F. D’Amico6P. Holder7A. Formigoni8DIMEVET, Dipartimento di Scienze Mediche Veterinarie, Università di Bologna, 40064 Ozzano Emilia, ItalyDIMEVET, Dipartimento di Scienze Mediche Veterinarie, Università di Bologna, 40064 Ozzano Emilia, ItalyDIMEVET, Dipartimento di Scienze Mediche Veterinarie, Università di Bologna, 40064 Ozzano Emilia, ItalyFencrest LLC, Holderness, NH 03245, USADIMEVET, Dipartimento di Scienze Mediche Veterinarie, Università di Bologna, 40064 Ozzano Emilia, ItalyUnit of Microbiome Science and Biotechnology, Department of Pharmacy and Biotechnology, University of Bologna, 40126 Bologna, ItalyUnit of Microbiomics, Department of Medical and Surgical Sciences, University of Bologna, 40138 Bologna, ItalyED&F Man Liquid Products, 3 London Bridge Street, London SE1 9SG, UKDIMEVET, Dipartimento di Scienze Mediche Veterinarie, Università di Bologna, 40064 Ozzano Emilia, ItalyThe aim of this study was to assess if molasses could modify VFA production and the rumen microbial community in vitro. Three beet (treatment Beet) and three cane (treatment Cane) molasses preparations were randomly selected from a variety of samples collected worldwide and incubated in vitro with rumen fluid along with a control sample (treatment CTR, in which no molasses was used). Flasks for VFA analysis were sampled at 0, 1, 2, 3, 4, 6, 8, and 24 h of each incubation. For microbiota analysis, samples from each fermentation flask after 12 and 24 h were subjected to microbial DNA extraction and V3–V4 16S rRNA gene sequencing on an Illumina MiSeq platform. Total net VFA production was higher in the beet and cane preparations than in the control (CTR) group at 24 h (33 mmol/L, 34 mmol/L, and 24.8 mmol/L, respectively), and the composition of VFAs was affected by the inclusion of molasses: acetic acid increased in the CTR group (73.5 mol%), while propionic acid increased in the beet and cane molasses (19.6 mol% and 18.6 mol%, respectively), and butyric acid increased, especially in the cane group (23.2 mol%). Molasses even influenced the composition of the rumen microbiota, and particularly the relative abundance of the most dominant family in the rumen, Prevotellaceae, which decreased compared to CTR (37.13%, 28.88%, and 49.6%, respectively). In contrast, Streptococcaceae (19.62% and 28.10% in molasses compared to 6.23% in CTR), Veillonellaceae (6.48% and 8.67% in molasses compared to 4.54% in CTR), and Fibrobacteraceae (0.90% and 0.88% in molasses compared to 0.62% in CTR) increased in the beet and cane groups compared to the CTR group. Another important finding is the lower proportion of Methanobacteriaceae following the addition of molasses compared to CTR (0.26%, 0.28%, and 0.43%, respectively). This study showed the impact of molasses in influencing VFA production and composition as a result of a modified rumen microbial composition.https://www.mdpi.com/2076-2615/13/4/728molassesVFAsrumen microbiotain vitro fermentation16S rRNA amplicon sequencing |
| spellingShingle | A. Palmonari A. Federiconi D. Cavallini C. J. Sniffen L. Mammi S. Turroni F. D’Amico P. Holder A. Formigoni Impact of Molasses on Ruminal Volatile Fatty Acid Production and Microbiota Composition In Vitro Animals molasses VFAs rumen microbiota in vitro fermentation 16S rRNA amplicon sequencing |
| title | Impact of Molasses on Ruminal Volatile Fatty Acid Production and Microbiota Composition In Vitro |
| title_full | Impact of Molasses on Ruminal Volatile Fatty Acid Production and Microbiota Composition In Vitro |
| title_fullStr | Impact of Molasses on Ruminal Volatile Fatty Acid Production and Microbiota Composition In Vitro |
| title_full_unstemmed | Impact of Molasses on Ruminal Volatile Fatty Acid Production and Microbiota Composition In Vitro |
| title_short | Impact of Molasses on Ruminal Volatile Fatty Acid Production and Microbiota Composition In Vitro |
| title_sort | impact of molasses on ruminal volatile fatty acid production and microbiota composition in vitro |
| topic | molasses VFAs rumen microbiota in vitro fermentation 16S rRNA amplicon sequencing |
| url | https://www.mdpi.com/2076-2615/13/4/728 |
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