Evaluation of the Antimicrobial Potential and Characterization of Novel T7-Like <i>Erwinia</i> Bacteriophages
The recent outbreak of blight in pome fruit plants has been a major concern as there are two indistinguishable <i>Erwinia</i> species, <i>Erwinia amylovora</i> and <i>E. pyrifoliae</i>, which cause blight in South Korea. Although there is a strict management proto...
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MDPI AG
2023-01-01
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| Online Access: | https://www.mdpi.com/2079-7737/12/2/180 |
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| author | Su Jin Jo Sang Guen Kim Young Min Lee Sib Sankar Giri Jeong Woo Kang Sung Bin Lee Won Joon Jung Mae Hyun Hwang Jaehong Park Chi Cheng Eunjung Roh Se Chang Park |
| author_facet | Su Jin Jo Sang Guen Kim Young Min Lee Sib Sankar Giri Jeong Woo Kang Sung Bin Lee Won Joon Jung Mae Hyun Hwang Jaehong Park Chi Cheng Eunjung Roh Se Chang Park |
| author_sort | Su Jin Jo |
| collection | DOAJ |
| description | The recent outbreak of blight in pome fruit plants has been a major concern as there are two indistinguishable <i>Erwinia</i> species, <i>Erwinia amylovora</i> and <i>E. pyrifoliae</i>, which cause blight in South Korea. Although there is a strict management protocol consisting of antibiotic-based prevention, the area and the number of cases of outbreaks have increased. In this study, we isolated four bacteriophages, pEp_SNUABM_03, 04, 11, and 12, that infect both <i>E. amylovora</i> and <i>E. pyrifoliae</i> and evaluated their potential as antimicrobial agents for administration against <i>Erwinia</i>-originated blight in South Korea. Morphological analysis revealed that all phages had podovirus-like capsids. The phage cocktail showed a broad spectrum of infectivity, infecting 98.91% of <i>E. amylovora</i> and 100% of <i>E. pyrifoliae</i> strains. The antibacterial effect was observed after long-term cocktail treatment against <i>E. amylovora</i>, whereas it was observed for both short- and long-term treatments against <i>E. pyrifoliae</i>. Genomic analysis verified that the phages did not encode harmful genes such as antibiotic resistance or virulence genes. All phages were stable under general orchard conditions. Collectively, we provided basic data on the potential of phages as biocontrol agents that target both <i>E. amylovora</i> and <i>E. pyrifoliae.</i> |
| first_indexed | 2024-03-11T09:08:25Z |
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| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-11T09:08:25Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-df6a2e963f1e4d08b191747a8a3dd7792023-11-16T19:12:43ZengMDPI AGBiology2079-77372023-01-0112218010.3390/biology12020180Evaluation of the Antimicrobial Potential and Characterization of Novel T7-Like <i>Erwinia</i> BacteriophagesSu Jin Jo0Sang Guen Kim1Young Min Lee2Sib Sankar Giri3Jeong Woo Kang4Sung Bin Lee5Won Joon Jung6Mae Hyun Hwang7Jaehong Park8Chi Cheng9Eunjung Roh10Se Chang Park11Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Republic of KoreaLaboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Republic of KoreaLaboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Republic of KoreaLaboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Republic of KoreaLaboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Republic of KoreaLaboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Republic of KoreaLaboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Republic of KoreaLaboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Republic of KoreaLaboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Republic of KoreaLaboratory of Aquatic Nutrition and Ecology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaCrop Protection Division, National Institute of Agriculture Sciences, Rural Development Administration, Wanju 55365, Republic of KoreaLaboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Republic of KoreaThe recent outbreak of blight in pome fruit plants has been a major concern as there are two indistinguishable <i>Erwinia</i> species, <i>Erwinia amylovora</i> and <i>E. pyrifoliae</i>, which cause blight in South Korea. Although there is a strict management protocol consisting of antibiotic-based prevention, the area and the number of cases of outbreaks have increased. In this study, we isolated four bacteriophages, pEp_SNUABM_03, 04, 11, and 12, that infect both <i>E. amylovora</i> and <i>E. pyrifoliae</i> and evaluated their potential as antimicrobial agents for administration against <i>Erwinia</i>-originated blight in South Korea. Morphological analysis revealed that all phages had podovirus-like capsids. The phage cocktail showed a broad spectrum of infectivity, infecting 98.91% of <i>E. amylovora</i> and 100% of <i>E. pyrifoliae</i> strains. The antibacterial effect was observed after long-term cocktail treatment against <i>E. amylovora</i>, whereas it was observed for both short- and long-term treatments against <i>E. pyrifoliae</i>. Genomic analysis verified that the phages did not encode harmful genes such as antibiotic resistance or virulence genes. All phages were stable under general orchard conditions. Collectively, we provided basic data on the potential of phages as biocontrol agents that target both <i>E. amylovora</i> and <i>E. pyrifoliae.</i>https://www.mdpi.com/2079-7737/12/2/180Bacteriophage<i>Erwinia</i> blightpome fruitphage cocktailagriculture |
| spellingShingle | Su Jin Jo Sang Guen Kim Young Min Lee Sib Sankar Giri Jeong Woo Kang Sung Bin Lee Won Joon Jung Mae Hyun Hwang Jaehong Park Chi Cheng Eunjung Roh Se Chang Park Evaluation of the Antimicrobial Potential and Characterization of Novel T7-Like <i>Erwinia</i> Bacteriophages Biology Bacteriophage <i>Erwinia</i> blight pome fruit phage cocktail agriculture |
| title | Evaluation of the Antimicrobial Potential and Characterization of Novel T7-Like <i>Erwinia</i> Bacteriophages |
| title_full | Evaluation of the Antimicrobial Potential and Characterization of Novel T7-Like <i>Erwinia</i> Bacteriophages |
| title_fullStr | Evaluation of the Antimicrobial Potential and Characterization of Novel T7-Like <i>Erwinia</i> Bacteriophages |
| title_full_unstemmed | Evaluation of the Antimicrobial Potential and Characterization of Novel T7-Like <i>Erwinia</i> Bacteriophages |
| title_short | Evaluation of the Antimicrobial Potential and Characterization of Novel T7-Like <i>Erwinia</i> Bacteriophages |
| title_sort | evaluation of the antimicrobial potential and characterization of novel t7 like i erwinia i bacteriophages |
| topic | Bacteriophage <i>Erwinia</i> blight pome fruit phage cocktail agriculture |
| url | https://www.mdpi.com/2079-7737/12/2/180 |
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