The Antioxidant Phytochemical Schisandrin A Promotes Neural Cell Proliferation and Differentiation after Ischemic Brain Injury
Schisandrin A (SCH) is a natural bioactive phytonutrient that belongs to the lignan derivatives found in <i>Schisandra chinensis</i> fruit. This study aims to investigate the impact of SCH on promoting neural progenitor cell (NPC) regeneration for avoiding stroke ischemic injury. The pro...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-12-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/26/24/7466 |
| _version_ | 1797502212916641792 |
|---|---|
| author | Wentian Zong Mostafa Gouda Enli Cai Ruofeng Wang Weijie Xu Yuming Wu Paulo E. S. Munekata José M. Lorenzo |
| author_facet | Wentian Zong Mostafa Gouda Enli Cai Ruofeng Wang Weijie Xu Yuming Wu Paulo E. S. Munekata José M. Lorenzo |
| author_sort | Wentian Zong |
| collection | DOAJ |
| description | Schisandrin A (SCH) is a natural bioactive phytonutrient that belongs to the lignan derivatives found in <i>Schisandra chinensis</i> fruit. This study aims to investigate the impact of SCH on promoting neural progenitor cell (NPC) regeneration for avoiding stroke ischemic injury. The promoting effect of SCH on NPCs was evaluated by photothrombotic model, immunofluorescence, cell line culture of NPCs, and Western blot assay. The results showed that neuron-specific class III beta-tubulin (Tuj1) was positive with Map2 positive nerve fibers in the ischemic area after using SCH. In addition, Nestin and SOX2 positive NPCs were significantly (<i>p</i> < 0.05) increased in the penumbra and core. Further analysis identified that SCH can regulate the expression level of cell division control protein 42 (Cdc42). In conclusion, our findings suggest that SCH enhanced NPCs proliferation and differentiation possible by Cdc42 to regulated cytoskeletal rearrangement and polarization of cells, which provides new hope for the late recovery of stroke. |
| first_indexed | 2024-03-10T03:29:44Z |
| format | Article |
| id | doaj.art-df6cb3a7e96346ef9900666ab46cd840 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T03:29:44Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-df6cb3a7e96346ef9900666ab46cd8402023-11-23T09:44:25ZengMDPI AGMolecules1420-30492021-12-012624746610.3390/molecules26247466The Antioxidant Phytochemical Schisandrin A Promotes Neural Cell Proliferation and Differentiation after Ischemic Brain InjuryWentian Zong0Mostafa Gouda1Enli Cai2Ruofeng Wang3Weijie Xu4Yuming Wu5Paulo E. S. Munekata6José M. Lorenzo7Kunming Health Vocational College, Kunming 650607, ChinaCollege of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, ChinaCollege of Nursing, Yunnan University of Chinese Medicine, Kunming 650500, ChinaCollege of health, Yunnan Technology and Business University, Kunming 651701, ChinaKunming Health Vocational College, Kunming 650607, ChinaCollege of Nursing, Yunnan University of Chinese Medicine, Kunming 650500, ChinaCentro Tecnológico de la Carne de Galicia, Rúa Galicia No. 4, Parque Tecnológico de Galicia, San Cibrao das Viñas, 32900 Ourense, SpainCentro Tecnológico de la Carne de Galicia, Rúa Galicia No. 4, Parque Tecnológico de Galicia, San Cibrao das Viñas, 32900 Ourense, SpainSchisandrin A (SCH) is a natural bioactive phytonutrient that belongs to the lignan derivatives found in <i>Schisandra chinensis</i> fruit. This study aims to investigate the impact of SCH on promoting neural progenitor cell (NPC) regeneration for avoiding stroke ischemic injury. The promoting effect of SCH on NPCs was evaluated by photothrombotic model, immunofluorescence, cell line culture of NPCs, and Western blot assay. The results showed that neuron-specific class III beta-tubulin (Tuj1) was positive with Map2 positive nerve fibers in the ischemic area after using SCH. In addition, Nestin and SOX2 positive NPCs were significantly (<i>p</i> < 0.05) increased in the penumbra and core. Further analysis identified that SCH can regulate the expression level of cell division control protein 42 (Cdc42). In conclusion, our findings suggest that SCH enhanced NPCs proliferation and differentiation possible by Cdc42 to regulated cytoskeletal rearrangement and polarization of cells, which provides new hope for the late recovery of stroke.https://www.mdpi.com/1420-3049/26/24/7466neural progenitor cells (NPCs)schisandrin Aischemic brain injurystrokeneural regenerationcell division control protein 42 (Cdc42) |
| spellingShingle | Wentian Zong Mostafa Gouda Enli Cai Ruofeng Wang Weijie Xu Yuming Wu Paulo E. S. Munekata José M. Lorenzo The Antioxidant Phytochemical Schisandrin A Promotes Neural Cell Proliferation and Differentiation after Ischemic Brain Injury Molecules neural progenitor cells (NPCs) schisandrin A ischemic brain injury stroke neural regeneration cell division control protein 42 (Cdc42) |
| title | The Antioxidant Phytochemical Schisandrin A Promotes Neural Cell Proliferation and Differentiation after Ischemic Brain Injury |
| title_full | The Antioxidant Phytochemical Schisandrin A Promotes Neural Cell Proliferation and Differentiation after Ischemic Brain Injury |
| title_fullStr | The Antioxidant Phytochemical Schisandrin A Promotes Neural Cell Proliferation and Differentiation after Ischemic Brain Injury |
| title_full_unstemmed | The Antioxidant Phytochemical Schisandrin A Promotes Neural Cell Proliferation and Differentiation after Ischemic Brain Injury |
| title_short | The Antioxidant Phytochemical Schisandrin A Promotes Neural Cell Proliferation and Differentiation after Ischemic Brain Injury |
| title_sort | antioxidant phytochemical schisandrin a promotes neural cell proliferation and differentiation after ischemic brain injury |
| topic | neural progenitor cells (NPCs) schisandrin A ischemic brain injury stroke neural regeneration cell division control protein 42 (Cdc42) |
| url | https://www.mdpi.com/1420-3049/26/24/7466 |
| work_keys_str_mv | AT wentianzong theantioxidantphytochemicalschisandrinapromotesneuralcellproliferationanddifferentiationafterischemicbraininjury AT mostafagouda theantioxidantphytochemicalschisandrinapromotesneuralcellproliferationanddifferentiationafterischemicbraininjury AT enlicai theantioxidantphytochemicalschisandrinapromotesneuralcellproliferationanddifferentiationafterischemicbraininjury AT ruofengwang theantioxidantphytochemicalschisandrinapromotesneuralcellproliferationanddifferentiationafterischemicbraininjury AT weijiexu theantioxidantphytochemicalschisandrinapromotesneuralcellproliferationanddifferentiationafterischemicbraininjury AT yumingwu theantioxidantphytochemicalschisandrinapromotesneuralcellproliferationanddifferentiationafterischemicbraininjury AT pauloesmunekata theantioxidantphytochemicalschisandrinapromotesneuralcellproliferationanddifferentiationafterischemicbraininjury AT josemlorenzo theantioxidantphytochemicalschisandrinapromotesneuralcellproliferationanddifferentiationafterischemicbraininjury AT wentianzong antioxidantphytochemicalschisandrinapromotesneuralcellproliferationanddifferentiationafterischemicbraininjury AT mostafagouda antioxidantphytochemicalschisandrinapromotesneuralcellproliferationanddifferentiationafterischemicbraininjury AT enlicai antioxidantphytochemicalschisandrinapromotesneuralcellproliferationanddifferentiationafterischemicbraininjury AT ruofengwang antioxidantphytochemicalschisandrinapromotesneuralcellproliferationanddifferentiationafterischemicbraininjury AT weijiexu antioxidantphytochemicalschisandrinapromotesneuralcellproliferationanddifferentiationafterischemicbraininjury AT yumingwu antioxidantphytochemicalschisandrinapromotesneuralcellproliferationanddifferentiationafterischemicbraininjury AT pauloesmunekata antioxidantphytochemicalschisandrinapromotesneuralcellproliferationanddifferentiationafterischemicbraininjury AT josemlorenzo antioxidantphytochemicalschisandrinapromotesneuralcellproliferationanddifferentiationafterischemicbraininjury |