Research Note. Transcriptomic study of the rat pinworm Syphacia muris
Syphacia muris is a ubiquitous nematode parasite and common contaminant of laboratory rats. A lthough S. muris infection is considered symptomless, it has some effects on the host’s immunity and therefore can interfere with experimental settings and interrupt final results. However, the molecular me...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Sciendo
2015-12-01
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| Series: | Helminthologia |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/helmin-2015-0059 |
| _version_ | 1827846981149523968 |
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| author | Okamoto M. Taira K. Ito R. Asai F. |
| author_facet | Okamoto M. Taira K. Ito R. Asai F. |
| author_sort | Okamoto M. |
| collection | DOAJ |
| description | Syphacia muris is a ubiquitous nematode parasite and common contaminant of laboratory rats. A lthough S. muris infection is considered symptomless, it has some effects on the host’s immunity and therefore can interfere with experimental settings and interrupt final results. However, the molecular mechanisms involved in the alteration within the host’s immunity remain unclear because of the absence of information about mRNA expressed in this parasite. In this study we performed the transcriptome profiling of S. muris by next-generation sequencing. After de novo assembly and annotation, 14,821 contigs were found to have a sequence homology with any nematode sequence. Gene ontology analysis showed that the majority of the expressed genes are involved in cellular process, binding, and catalytic activity. Although the rate of expressed genes involved in the immune system was low, we found candidate genes that might be involved in the alteration within the host’s immunity by regulating the host’s innate immune response. |
| first_indexed | 2024-03-12T09:24:44Z |
| format | Article |
| id | doaj.art-df6d7b140a5e41b8bf3ef2a87908878c |
| institution | Directory Open Access Journal |
| issn | 0440-6605 1336-9083 |
| language | English |
| last_indexed | 2024-03-12T09:24:44Z |
| publishDate | 2015-12-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Helminthologia |
| spelling | doaj.art-df6d7b140a5e41b8bf3ef2a87908878c2023-09-02T14:21:52ZengSciendoHelminthologia0440-66051336-90832015-12-0152437037410.1515/helmin-2015-0059helmin-2015-0059Research Note. Transcriptomic study of the rat pinworm Syphacia murisOkamoto M.0Taira K.1Ito R.2Asai F.3Laboratory of Veterinary Immunology, School of Veterinary Medicine, Azabu University 1-17-71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa 252-5201, JapanLaboratory of Parasitology, School of Veterinary Medicine, Azabu University 1-17-71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa 252-5201, JapanLaboratory of Veterinary Immunology, School of Veterinary Medicine, Azabu University 1-17-71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa 252-5201, JapanLaboratory of Veterinary Pharmacology, School of Veterinary Medicine, Azabu University 1-17-71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa 252-5201, JapanSyphacia muris is a ubiquitous nematode parasite and common contaminant of laboratory rats. A lthough S. muris infection is considered symptomless, it has some effects on the host’s immunity and therefore can interfere with experimental settings and interrupt final results. However, the molecular mechanisms involved in the alteration within the host’s immunity remain unclear because of the absence of information about mRNA expressed in this parasite. In this study we performed the transcriptome profiling of S. muris by next-generation sequencing. After de novo assembly and annotation, 14,821 contigs were found to have a sequence homology with any nematode sequence. Gene ontology analysis showed that the majority of the expressed genes are involved in cellular process, binding, and catalytic activity. Although the rate of expressed genes involved in the immune system was low, we found candidate genes that might be involved in the alteration within the host’s immunity by regulating the host’s innate immune response.https://doi.org/10.1515/helmin-2015-0059syphacia muristranscriptomegene ontology analysis |
| spellingShingle | Okamoto M. Taira K. Ito R. Asai F. Research Note. Transcriptomic study of the rat pinworm Syphacia muris Helminthologia syphacia muris transcriptome gene ontology analysis |
| title | Research Note. Transcriptomic study of the rat pinworm Syphacia muris |
| title_full | Research Note. Transcriptomic study of the rat pinworm Syphacia muris |
| title_fullStr | Research Note. Transcriptomic study of the rat pinworm Syphacia muris |
| title_full_unstemmed | Research Note. Transcriptomic study of the rat pinworm Syphacia muris |
| title_short | Research Note. Transcriptomic study of the rat pinworm Syphacia muris |
| title_sort | research note transcriptomic study of the rat pinworm syphacia muris |
| topic | syphacia muris transcriptome gene ontology analysis |
| url | https://doi.org/10.1515/helmin-2015-0059 |
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