Comprehensive analysis of the LHT gene family in tobacco and functional characterization of NtLHT22 involvement in amino acids homeostasis
Amino acids are vital nitrogen (N) sources for plant growth, development, and yield. The uptake and translocation of amino acids are mediated by amino acid transporters (AATs). The AATs family including lysine-histidine transporters (LHTs), amino acid permeases (AAPs), and proline transporters (ProT...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.927844/full |
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| author | Zhaowu Li Zhaowu Li Junping Gao Shuaibin Wang Xiaodong Xie Zhangying Wang Yu Peng Xiaonian Yang Wenxuan Pu Yaofu Wang Xiaorong Fan |
| author_facet | Zhaowu Li Zhaowu Li Junping Gao Shuaibin Wang Xiaodong Xie Zhangying Wang Yu Peng Xiaonian Yang Wenxuan Pu Yaofu Wang Xiaorong Fan |
| author_sort | Zhaowu Li |
| collection | DOAJ |
| description | Amino acids are vital nitrogen (N) sources for plant growth, development, and yield. The uptake and translocation of amino acids are mediated by amino acid transporters (AATs). The AATs family including lysine-histidine transporters (LHTs), amino acid permeases (AAPs), and proline transporters (ProTs) subfamilies have been identified in various plants. However, little is known about these genes in tobacco. In this study, we identified 23 LHT genes, the important members of AATs, in the tobacco genome. The gene structure, phylogenetic tree, transmembrane helices, chromosomal distribution, cis-regulatory elements, and expression profiles of NtLHT genes were systematically analyzed. Phylogenetic analysis divided the 23 NtLHT genes into two conserved subgroups. Expression profiles confirmed that the NtLHT genes were differentially expressed in various tissues, indicating their potential roles in tobacco growth and development. Cis-elements analysis of promoters and expression patterns after stress treatments suggested that NtLHT genes probable participate in abiotic stress responses of tobacco. In addition, Knock out and overexpression of NtLHT22 changed the amino acids homeostasis in the transgenic plants, the contents of amino acids were significantly decreased in NtLHT22 overexpression plants than wild-type. The results from this study provide important information for further studies on the molecular functions of the NtLHT genes. |
| first_indexed | 2024-12-10T14:47:30Z |
| format | Article |
| id | doaj.art-df6d9c01fdb04e538c9662d5bce804e4 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-10T14:47:30Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-df6d9c01fdb04e538c9662d5bce804e42022-12-22T01:44:32ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-09-011310.3389/fpls.2022.927844927844Comprehensive analysis of the LHT gene family in tobacco and functional characterization of NtLHT22 involvement in amino acids homeostasisZhaowu Li0Zhaowu Li1Junping Gao2Shuaibin Wang3Xiaodong Xie4Zhangying Wang5Yu Peng6Xiaonian Yang7Wenxuan Pu8Yaofu Wang9Xiaorong Fan10Tobacco Research Institute of Technology Centre, China Tobacco Hunan Industrial Corporation, Changsha, ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Ministry of Agriculture Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Nanjing Agricultural University, Nanjing, ChinaTobacco Research Institute of Technology Centre, China Tobacco Hunan Industrial Corporation, Changsha, ChinaTobacco Research Institute of Technology Centre, China Tobacco Hunan Industrial Corporation, Changsha, ChinaChina Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of China National Tobacco Corporation, Zhengzhou, ChinaState Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, ChinaTobacco Research Institute of Technology Centre, China Tobacco Hunan Industrial Corporation, Changsha, ChinaTobacco Research Institute of Technology Centre, China Tobacco Hunan Industrial Corporation, Changsha, ChinaTobacco Research Institute of Technology Centre, China Tobacco Hunan Industrial Corporation, Changsha, ChinaTobacco Research Institute of Technology Centre, China Tobacco Hunan Industrial Corporation, Changsha, ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Ministry of Agriculture Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Nanjing Agricultural University, Nanjing, ChinaAmino acids are vital nitrogen (N) sources for plant growth, development, and yield. The uptake and translocation of amino acids are mediated by amino acid transporters (AATs). The AATs family including lysine-histidine transporters (LHTs), amino acid permeases (AAPs), and proline transporters (ProTs) subfamilies have been identified in various plants. However, little is known about these genes in tobacco. In this study, we identified 23 LHT genes, the important members of AATs, in the tobacco genome. The gene structure, phylogenetic tree, transmembrane helices, chromosomal distribution, cis-regulatory elements, and expression profiles of NtLHT genes were systematically analyzed. Phylogenetic analysis divided the 23 NtLHT genes into two conserved subgroups. Expression profiles confirmed that the NtLHT genes were differentially expressed in various tissues, indicating their potential roles in tobacco growth and development. Cis-elements analysis of promoters and expression patterns after stress treatments suggested that NtLHT genes probable participate in abiotic stress responses of tobacco. In addition, Knock out and overexpression of NtLHT22 changed the amino acids homeostasis in the transgenic plants, the contents of amino acids were significantly decreased in NtLHT22 overexpression plants than wild-type. The results from this study provide important information for further studies on the molecular functions of the NtLHT genes.https://www.frontiersin.org/articles/10.3389/fpls.2022.927844/fullNicotiana tabacumLHTbioinformaticsexpression profileamino acids |
| spellingShingle | Zhaowu Li Zhaowu Li Junping Gao Shuaibin Wang Xiaodong Xie Zhangying Wang Yu Peng Xiaonian Yang Wenxuan Pu Yaofu Wang Xiaorong Fan Comprehensive analysis of the LHT gene family in tobacco and functional characterization of NtLHT22 involvement in amino acids homeostasis Frontiers in Plant Science Nicotiana tabacum LHT bioinformatics expression profile amino acids |
| title | Comprehensive analysis of the LHT gene family in tobacco and functional characterization of NtLHT22 involvement in amino acids homeostasis |
| title_full | Comprehensive analysis of the LHT gene family in tobacco and functional characterization of NtLHT22 involvement in amino acids homeostasis |
| title_fullStr | Comprehensive analysis of the LHT gene family in tobacco and functional characterization of NtLHT22 involvement in amino acids homeostasis |
| title_full_unstemmed | Comprehensive analysis of the LHT gene family in tobacco and functional characterization of NtLHT22 involvement in amino acids homeostasis |
| title_short | Comprehensive analysis of the LHT gene family in tobacco and functional characterization of NtLHT22 involvement in amino acids homeostasis |
| title_sort | comprehensive analysis of the lht gene family in tobacco and functional characterization of ntlht22 involvement in amino acids homeostasis |
| topic | Nicotiana tabacum LHT bioinformatics expression profile amino acids |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.927844/full |
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