The Inactivated ISKNV-I Vaccine Confers Highly Effective Cross-Protection against Epidemic RSIV-I and RSIV-II from Cultured Spotted Sea Bass Lateolabrax maculatus
ABSTRACT The genus Megalocytivirus of the family Iridoviridae is composed of two distinct species, namely, infectious spleen and kidney necrosis virus (ISKNV) and scale drop disease virus (SDDV), and both are important causative agents in a variety of bony fish worldwide. Of them, the ISKNV species...
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| Language: | English |
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American Society for Microbiology
2023-06-01
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| Series: | Microbiology Spectrum |
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| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.04495-22 |
| _version_ | 1797803497052176384 |
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| author | Weixuan Fu Yong Li Yuting Fu Wenfeng Zhang Panpan Luo Qianqian Sun Fangzhao Yu Shaoping Weng Wangdong Li Jianguo He Chuanfu Dong |
| author_facet | Weixuan Fu Yong Li Yuting Fu Wenfeng Zhang Panpan Luo Qianqian Sun Fangzhao Yu Shaoping Weng Wangdong Li Jianguo He Chuanfu Dong |
| author_sort | Weixuan Fu |
| collection | DOAJ |
| description | ABSTRACT The genus Megalocytivirus of the family Iridoviridae is composed of two distinct species, namely, infectious spleen and kidney necrosis virus (ISKNV) and scale drop disease virus (SDDV), and both are important causative agents in a variety of bony fish worldwide. Of them, the ISKNV species is subdivided into three genotypes, namely, red seabream iridovirus (RSIV), ISKNV, and turbot reddish body iridovirus (TRBIV), and a further six subgenotypes, RSIV-I, RSIV-II, ISKNV-I, ISKNV-II, TRBIV-I, and TRBIV-II. Commercial vaccines derived from RSIV-I , RSIV-II and ISKNV-I have been available to several fish species. However, studies regarding the cross-protection effect among different genotype or subgenotype isolates have not been fully elucidated. In this study, RSIV-I and RSIV-II were demonstrated as the causative agents in cultured spotted seabass, Lateolabrax maculatus, through serial robust evidence, including cell culture-based viral isolation, whole-genome determination and phylogeny analysis, artificial challenge, histopathology, immunohistochemistry, and immunofluorescence as well as transmission electron microscope observation. Thereafter, a formalin-killed cell (FKC) vaccine generated from an ISKNV-I isolate was prepared to evaluate the protective effects against two spotted seabass original RSIV-I and RSIV-II. The result showed that the ISKNV-I-based FKC vaccine conferred almost complete cross-protection against RSIV-I and RSIV-II as well as ISKNV-I itself. No serotype difference was observed among RSIV-I, RSIV-II, and ISKNV-I. Additionally, the mandarin fish Siniperca chuatsi is proposed as an ideal infection and vaccination fish species for the study of various megalocytiviral isolates. IMPORTANCE Red seabream iridovirus (RSIV) infects a wide mariculture bony fish and has resulted in significant annual economic loss worldwide. Previous studies showed that the phenotypic diversity of infectious RSIV isolates would lead to different virulence characteristics, viral antigenicity, and vaccine efficacy as well as host range. Importantly, it is still doubted whether a universal vaccine could confer the same highly protective effect against various genotypic isolates. Our study here presented enough experimental evidence that a water in oil (w/o) formation of inactivated ISKNV-I vaccine could confer almost complete protection against RSIV-I and RSIV-II as well as ISKNV-I itself. Our study provides valuable data for better understanding the differential infection and immunity among different genotypes of ISKNV and RSIV isolates in the genus Megalocytivirus. |
| first_indexed | 2024-03-13T05:21:45Z |
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| id | doaj.art-df6dfef09a7243ebb53fa7de403c02ab |
| institution | Directory Open Access Journal |
| issn | 2165-0497 |
| language | English |
| last_indexed | 2024-03-13T05:21:45Z |
| publishDate | 2023-06-01 |
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| spelling | doaj.art-df6dfef09a7243ebb53fa7de403c02ab2023-06-15T13:18:30ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972023-06-0111310.1128/spectrum.04495-22The Inactivated ISKNV-I Vaccine Confers Highly Effective Cross-Protection against Epidemic RSIV-I and RSIV-II from Cultured Spotted Sea Bass Lateolabrax maculatusWeixuan Fu0Yong Li1Yuting Fu2Wenfeng Zhang3Panpan Luo4Qianqian Sun5Fangzhao Yu6Shaoping Weng7Wangdong Li8Jianguo He9Chuanfu Dong10State Key Laboratory of Biocontrol (Guangzhou, SYSU)/Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai, SMST-GDL), School of Life Sciences of Sun Yat-sen University, Guangzhou, ChinaZhuhai Modern Agriculture Development Center, Zhuhai, ChinaState Key Laboratory of Biocontrol (Guangzhou, SYSU)/Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai, SMST-GDL), School of Life Sciences of Sun Yat-sen University, Guangzhou, ChinaState Key Laboratory of Biocontrol (Guangzhou, SYSU)/Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai, SMST-GDL), School of Life Sciences of Sun Yat-sen University, Guangzhou, ChinaState Key Laboratory of Biocontrol (Guangzhou, SYSU)/Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai, SMST-GDL), School of Life Sciences of Sun Yat-sen University, Guangzhou, ChinaState Key Laboratory of Biocontrol (Guangzhou, SYSU)/Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai, SMST-GDL), School of Life Sciences of Sun Yat-sen University, Guangzhou, ChinaZhuhai Modern Agriculture Development Center, Zhuhai, ChinaState Key Laboratory of Biocontrol (Guangzhou, SYSU)/Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai, SMST-GDL), School of Life Sciences of Sun Yat-sen University, Guangzhou, ChinaZhuhai Modern Agriculture Development Center, Zhuhai, ChinaState Key Laboratory of Biocontrol (Guangzhou, SYSU)/Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai, SMST-GDL), School of Life Sciences of Sun Yat-sen University, Guangzhou, ChinaState Key Laboratory of Biocontrol (Guangzhou, SYSU)/Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai, SMST-GDL), School of Life Sciences of Sun Yat-sen University, Guangzhou, ChinaABSTRACT The genus Megalocytivirus of the family Iridoviridae is composed of two distinct species, namely, infectious spleen and kidney necrosis virus (ISKNV) and scale drop disease virus (SDDV), and both are important causative agents in a variety of bony fish worldwide. Of them, the ISKNV species is subdivided into three genotypes, namely, red seabream iridovirus (RSIV), ISKNV, and turbot reddish body iridovirus (TRBIV), and a further six subgenotypes, RSIV-I, RSIV-II, ISKNV-I, ISKNV-II, TRBIV-I, and TRBIV-II. Commercial vaccines derived from RSIV-I , RSIV-II and ISKNV-I have been available to several fish species. However, studies regarding the cross-protection effect among different genotype or subgenotype isolates have not been fully elucidated. In this study, RSIV-I and RSIV-II were demonstrated as the causative agents in cultured spotted seabass, Lateolabrax maculatus, through serial robust evidence, including cell culture-based viral isolation, whole-genome determination and phylogeny analysis, artificial challenge, histopathology, immunohistochemistry, and immunofluorescence as well as transmission electron microscope observation. Thereafter, a formalin-killed cell (FKC) vaccine generated from an ISKNV-I isolate was prepared to evaluate the protective effects against two spotted seabass original RSIV-I and RSIV-II. The result showed that the ISKNV-I-based FKC vaccine conferred almost complete cross-protection against RSIV-I and RSIV-II as well as ISKNV-I itself. No serotype difference was observed among RSIV-I, RSIV-II, and ISKNV-I. Additionally, the mandarin fish Siniperca chuatsi is proposed as an ideal infection and vaccination fish species for the study of various megalocytiviral isolates. IMPORTANCE Red seabream iridovirus (RSIV) infects a wide mariculture bony fish and has resulted in significant annual economic loss worldwide. Previous studies showed that the phenotypic diversity of infectious RSIV isolates would lead to different virulence characteristics, viral antigenicity, and vaccine efficacy as well as host range. Importantly, it is still doubted whether a universal vaccine could confer the same highly protective effect against various genotypic isolates. Our study here presented enough experimental evidence that a water in oil (w/o) formation of inactivated ISKNV-I vaccine could confer almost complete protection against RSIV-I and RSIV-II as well as ISKNV-I itself. Our study provides valuable data for better understanding the differential infection and immunity among different genotypes of ISKNV and RSIV isolates in the genus Megalocytivirus.https://journals.asm.org/doi/10.1128/spectrum.04495-22MegalocytivirusRSIV genotypeISKNV genotypeinactivated vaccinecross-protectioninfectious spleen and kidney necrosis virus |
| spellingShingle | Weixuan Fu Yong Li Yuting Fu Wenfeng Zhang Panpan Luo Qianqian Sun Fangzhao Yu Shaoping Weng Wangdong Li Jianguo He Chuanfu Dong The Inactivated ISKNV-I Vaccine Confers Highly Effective Cross-Protection against Epidemic RSIV-I and RSIV-II from Cultured Spotted Sea Bass Lateolabrax maculatus Microbiology Spectrum Megalocytivirus RSIV genotype ISKNV genotype inactivated vaccine cross-protection infectious spleen and kidney necrosis virus |
| title | The Inactivated ISKNV-I Vaccine Confers Highly Effective Cross-Protection against Epidemic RSIV-I and RSIV-II from Cultured Spotted Sea Bass Lateolabrax maculatus |
| title_full | The Inactivated ISKNV-I Vaccine Confers Highly Effective Cross-Protection against Epidemic RSIV-I and RSIV-II from Cultured Spotted Sea Bass Lateolabrax maculatus |
| title_fullStr | The Inactivated ISKNV-I Vaccine Confers Highly Effective Cross-Protection against Epidemic RSIV-I and RSIV-II from Cultured Spotted Sea Bass Lateolabrax maculatus |
| title_full_unstemmed | The Inactivated ISKNV-I Vaccine Confers Highly Effective Cross-Protection against Epidemic RSIV-I and RSIV-II from Cultured Spotted Sea Bass Lateolabrax maculatus |
| title_short | The Inactivated ISKNV-I Vaccine Confers Highly Effective Cross-Protection against Epidemic RSIV-I and RSIV-II from Cultured Spotted Sea Bass Lateolabrax maculatus |
| title_sort | inactivated isknv i vaccine confers highly effective cross protection against epidemic rsiv i and rsiv ii from cultured spotted sea bass lateolabrax maculatus |
| topic | Megalocytivirus RSIV genotype ISKNV genotype inactivated vaccine cross-protection infectious spleen and kidney necrosis virus |
| url | https://journals.asm.org/doi/10.1128/spectrum.04495-22 |
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