Saikosaponin D inhibits nasal inflammation by regulating the transcription factors T-box protein expressed in T cells/GATA-3 and retinoic acid-related orphan nuclear receptor γt in a murine model of allergic rhinitis
Context: Saikosaponin D (SSD) is a commonly prescribed agent against inflammatory diseases in Asian countries. However, the anti-allergic inflammatory effect of SSD in allergic rhinitis (AR) model is not well known. Objective: We investigated the anti-allergic and anti-inflammatory effects of SSD on...
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| Language: | English |
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Elsevier
2023-06-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023045279 |
| _version_ | 1797799995705917440 |
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| author | Chun Hua Piaoa Shen Chun Zou Thi Tho Bui Chang Ho Song Ok Hee Chai |
| author_facet | Chun Hua Piaoa Shen Chun Zou Thi Tho Bui Chang Ho Song Ok Hee Chai |
| author_sort | Chun Hua Piaoa |
| collection | DOAJ |
| description | Context: Saikosaponin D (SSD) is a commonly prescribed agent against inflammatory diseases in Asian countries. However, the anti-allergic inflammatory effect of SSD in allergic rhinitis (AR) model is not well known. Objective: We investigated the anti-allergic and anti-inflammatory effects of SSD on the ovalbumin (OVA)-induced AR model. Materials and method: BALB/c mice were divided into the control, OVA, OVA + SSD, and OVA + dexamethasone (Dex) groups. AR was established by intraperitoneal injection with OVA adsorbed to aluminum hydroxide, and intranasal challenge with OVA. Thereafter, the mice were treated with 10 mg/kg BW (Body weight) of OVA + SSD and 2.5 mg/kg BW of Dex orally for 11 days before being challenged. Subsequently, the mice were challenged with OVA 1 h after SSD or Dex treatment. The Control group was treated with saline only. Results: The addition of 10 mg/kg BW of OVA + SSD significantly ameliorated the nasal symptoms including sneezing and rubbing from 30 ± 5.2 times in OVA group to 20 ± 5.8 times. Moreover, OVA + SSD group decreased the production of TNF-α, IL-4, IL-5, IL-17, GATA-3 and RORγ about 1.2–1.4-fold compared to the OVA-induced AR mice near to 2.5 mg/kg BW of Dex levels. Meanwhile OVA + SSD group slightly increased the levels of INF-γ, IL-12 and T-bet about 1.8–2.0-fold compared to the OVA group near to control group. Notably, OVA + SSD group also reduced the levels of OVA-specific IgE and IgG1 about 0.5–2.5-fold compared OVA group but increased the levels of IgG2a in serum. The results were analyzed using Graph Pad Prism software (v5.0, La Jolla, CA, USA). Conclusion: SSD may represent an alternative therapeutic approach for the treatment of patients with AR through the regulation of transcription factors T-bet, GATA-3, and RORγ in inflammatory cells. |
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| format | Article |
| id | doaj.art-df7151d860c644448fb3db373d214e73 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-13T04:28:22Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
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| series | Heliyon |
| spelling | doaj.art-df7151d860c644448fb3db373d214e732023-06-20T04:20:10ZengElsevierHeliyon2405-84402023-06-0196e17319Saikosaponin D inhibits nasal inflammation by regulating the transcription factors T-box protein expressed in T cells/GATA-3 and retinoic acid-related orphan nuclear receptor γt in a murine model of allergic rhinitisChun Hua Piaoa0Shen Chun Zou1Thi Tho Bui2Chang Ho Song3Ok Hee Chai4Department of Anatomy, Jeonbuk National University Medical School, Jeonju, 54907, Republic of KoreaDepartment of Pulmonary and Critical Care Medicine, Yantai Yuhuangding Hospital, Yantai, Shandong 264000, PR ChinaDepartment of Anatomy, Jeonbuk National University Medical School, Jeonju, 54907, Republic of Korea; Faculty of Biology & Environmental Science, University of Science and Education, The University of Danang, Danang 555940, Viet NamDepartment of Anatomy, Jeonbuk National University Medical School, Jeonju, 54907, Republic of Korea; Institute for Medical Sciences, Jeonbuk National University, Jeonju, 54907, Republic of KoreaDepartment of Anatomy, Jeonbuk National University Medical School, Jeonju, 54907, Republic of Korea; Institute for Medical Sciences, Jeonbuk National University, Jeonju, 54907, Republic of Korea; Corresponding author. Department of Anatomy, Jeonbuk National University Medical School, Jeonju, 54907, Republic of Korea.Context: Saikosaponin D (SSD) is a commonly prescribed agent against inflammatory diseases in Asian countries. However, the anti-allergic inflammatory effect of SSD in allergic rhinitis (AR) model is not well known. Objective: We investigated the anti-allergic and anti-inflammatory effects of SSD on the ovalbumin (OVA)-induced AR model. Materials and method: BALB/c mice were divided into the control, OVA, OVA + SSD, and OVA + dexamethasone (Dex) groups. AR was established by intraperitoneal injection with OVA adsorbed to aluminum hydroxide, and intranasal challenge with OVA. Thereafter, the mice were treated with 10 mg/kg BW (Body weight) of OVA + SSD and 2.5 mg/kg BW of Dex orally for 11 days before being challenged. Subsequently, the mice were challenged with OVA 1 h after SSD or Dex treatment. The Control group was treated with saline only. Results: The addition of 10 mg/kg BW of OVA + SSD significantly ameliorated the nasal symptoms including sneezing and rubbing from 30 ± 5.2 times in OVA group to 20 ± 5.8 times. Moreover, OVA + SSD group decreased the production of TNF-α, IL-4, IL-5, IL-17, GATA-3 and RORγ about 1.2–1.4-fold compared to the OVA-induced AR mice near to 2.5 mg/kg BW of Dex levels. Meanwhile OVA + SSD group slightly increased the levels of INF-γ, IL-12 and T-bet about 1.8–2.0-fold compared to the OVA group near to control group. Notably, OVA + SSD group also reduced the levels of OVA-specific IgE and IgG1 about 0.5–2.5-fold compared OVA group but increased the levels of IgG2a in serum. The results were analyzed using Graph Pad Prism software (v5.0, La Jolla, CA, USA). Conclusion: SSD may represent an alternative therapeutic approach for the treatment of patients with AR through the regulation of transcription factors T-bet, GATA-3, and RORγ in inflammatory cells.http://www.sciencedirect.com/science/article/pii/S2405844023045279SSDAnti-allergic inflammatory effectCytokinesTh1Th2Th17 |
| spellingShingle | Chun Hua Piaoa Shen Chun Zou Thi Tho Bui Chang Ho Song Ok Hee Chai Saikosaponin D inhibits nasal inflammation by regulating the transcription factors T-box protein expressed in T cells/GATA-3 and retinoic acid-related orphan nuclear receptor γt in a murine model of allergic rhinitis Heliyon SSD Anti-allergic inflammatory effect Cytokines Th1 Th2 Th17 |
| title | Saikosaponin D inhibits nasal inflammation by regulating the transcription factors T-box protein expressed in T cells/GATA-3 and retinoic acid-related orphan nuclear receptor γt in a murine model of allergic rhinitis |
| title_full | Saikosaponin D inhibits nasal inflammation by regulating the transcription factors T-box protein expressed in T cells/GATA-3 and retinoic acid-related orphan nuclear receptor γt in a murine model of allergic rhinitis |
| title_fullStr | Saikosaponin D inhibits nasal inflammation by regulating the transcription factors T-box protein expressed in T cells/GATA-3 and retinoic acid-related orphan nuclear receptor γt in a murine model of allergic rhinitis |
| title_full_unstemmed | Saikosaponin D inhibits nasal inflammation by regulating the transcription factors T-box protein expressed in T cells/GATA-3 and retinoic acid-related orphan nuclear receptor γt in a murine model of allergic rhinitis |
| title_short | Saikosaponin D inhibits nasal inflammation by regulating the transcription factors T-box protein expressed in T cells/GATA-3 and retinoic acid-related orphan nuclear receptor γt in a murine model of allergic rhinitis |
| title_sort | saikosaponin d inhibits nasal inflammation by regulating the transcription factors t box protein expressed in t cells gata 3 and retinoic acid related orphan nuclear receptor γt in a murine model of allergic rhinitis |
| topic | SSD Anti-allergic inflammatory effect Cytokines Th1 Th2 Th17 |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023045279 |
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