HRV16 Impairs Macrophages Cytokine Response to a Secondary Bacterial Trigger
Human rhinovirus is frequently seen as an upper respiratory tract infection but growing evidence proves the virus can cause lower respiratory tract infections in patients with chronic inflammatory lung diseases including chronic obstructive pulmonary disease (COPD). In addition to airway epithelial...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-12-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fimmu.2018.02908/full |
| _version_ | 1818989325825605632 |
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| author | Jamil Jubrail Jamil Jubrail Jamil Jubrail Kshanti Africano-Gomez Kshanti Africano-Gomez Kshanti Africano-Gomez Floriane Herit Floriane Herit Floriane Herit Engin Baturcam Gaell Mayer Danen Mootoosamy Cunoosamy Nisha Kurian Florence Niedergang Florence Niedergang Florence Niedergang |
| author_facet | Jamil Jubrail Jamil Jubrail Jamil Jubrail Kshanti Africano-Gomez Kshanti Africano-Gomez Kshanti Africano-Gomez Floriane Herit Floriane Herit Floriane Herit Engin Baturcam Gaell Mayer Danen Mootoosamy Cunoosamy Nisha Kurian Florence Niedergang Florence Niedergang Florence Niedergang |
| author_sort | Jamil Jubrail |
| collection | DOAJ |
| description | Human rhinovirus is frequently seen as an upper respiratory tract infection but growing evidence proves the virus can cause lower respiratory tract infections in patients with chronic inflammatory lung diseases including chronic obstructive pulmonary disease (COPD). In addition to airway epithelial cells, macrophages are crucial for regulating inflammatory responses to viral infections. However, the response of macrophages to HRV has not been analyzed in detail. We used in vitro monocyte-derived human macrophages to study the cytokine secretion of macrophages in response to the virus. Our results showed that macrophages were competent at responding to HRV, as a robust cytokine response was detected. However, after subsequent exposure to non-typeable Haemophilus influenzae (NTHi) or to LPS, HRV-treated macrophages secreted reduced levels of pro-inflammatory or regulatory cytokines. This “paralyzed” phenotype was not mimicked if the macrophages were pre-treated with LPS or CpG instead of the virus. These results begin to deepen our understanding into why patients with COPD show HRV-induced exacerbations and why they mount a defective response toward NTHi. |
| first_indexed | 2024-12-20T19:36:41Z |
| format | Article |
| id | doaj.art-df7978d8fd3b44e0a3768d27e84ca17a |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-20T19:36:41Z |
| publishDate | 2018-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-df7978d8fd3b44e0a3768d27e84ca17a2022-12-21T19:28:38ZengFrontiers Media S.A.Frontiers in Immunology1664-32242018-12-01910.3389/fimmu.2018.02908412754HRV16 Impairs Macrophages Cytokine Response to a Secondary Bacterial TriggerJamil Jubrail0Jamil Jubrail1Jamil Jubrail2Kshanti Africano-Gomez3Kshanti Africano-Gomez4Kshanti Africano-Gomez5Floriane Herit6Floriane Herit7Floriane Herit8Engin Baturcam9Gaell Mayer10Danen Mootoosamy Cunoosamy11Nisha Kurian12Florence Niedergang13Florence Niedergang14Florence Niedergang15Institut Cochin, Inserm U1016, Paris, FranceCNRS, UMR 8104, Paris, FranceUniversité Paris Descartes, Sorbonne Paris Cité, Paris, FranceInstitut Cochin, Inserm U1016, Paris, FranceCNRS, UMR 8104, Paris, FranceUniversité Paris Descartes, Sorbonne Paris Cité, Paris, FranceInstitut Cochin, Inserm U1016, Paris, FranceCNRS, UMR 8104, Paris, FranceUniversité Paris Descartes, Sorbonne Paris Cité, Paris, FranceIMED Biotech Unit, Target and Translational Science, Respiratory, Inflammation & Autoimmunity, AstraZeneca, Gothenburg, SwedenClinical Development, Respiratory Inhalation & Oral Development, GMD, AstraZeneca, Gothenburg, SwedenIMED Biotech Unit, Target and Translational Science, Respiratory, Inflammation & Autoimmunity, AstraZeneca, Gothenburg, SwedenPrecision Medicine & Genomics, IMED Biotech Unit, AstraZeneca, Gothenburg, SwedenInstitut Cochin, Inserm U1016, Paris, FranceCNRS, UMR 8104, Paris, FranceUniversité Paris Descartes, Sorbonne Paris Cité, Paris, FranceHuman rhinovirus is frequently seen as an upper respiratory tract infection but growing evidence proves the virus can cause lower respiratory tract infections in patients with chronic inflammatory lung diseases including chronic obstructive pulmonary disease (COPD). In addition to airway epithelial cells, macrophages are crucial for regulating inflammatory responses to viral infections. However, the response of macrophages to HRV has not been analyzed in detail. We used in vitro monocyte-derived human macrophages to study the cytokine secretion of macrophages in response to the virus. Our results showed that macrophages were competent at responding to HRV, as a robust cytokine response was detected. However, after subsequent exposure to non-typeable Haemophilus influenzae (NTHi) or to LPS, HRV-treated macrophages secreted reduced levels of pro-inflammatory or regulatory cytokines. This “paralyzed” phenotype was not mimicked if the macrophages were pre-treated with LPS or CpG instead of the virus. These results begin to deepen our understanding into why patients with COPD show HRV-induced exacerbations and why they mount a defective response toward NTHi.https://www.frontiersin.org/article/10.3389/fimmu.2018.02908/fullmacrophagerhinovirusphagocytosiscytokinebacteria |
| spellingShingle | Jamil Jubrail Jamil Jubrail Jamil Jubrail Kshanti Africano-Gomez Kshanti Africano-Gomez Kshanti Africano-Gomez Floriane Herit Floriane Herit Floriane Herit Engin Baturcam Gaell Mayer Danen Mootoosamy Cunoosamy Nisha Kurian Florence Niedergang Florence Niedergang Florence Niedergang HRV16 Impairs Macrophages Cytokine Response to a Secondary Bacterial Trigger Frontiers in Immunology macrophage rhinovirus phagocytosis cytokine bacteria |
| title | HRV16 Impairs Macrophages Cytokine Response to a Secondary Bacterial Trigger |
| title_full | HRV16 Impairs Macrophages Cytokine Response to a Secondary Bacterial Trigger |
| title_fullStr | HRV16 Impairs Macrophages Cytokine Response to a Secondary Bacterial Trigger |
| title_full_unstemmed | HRV16 Impairs Macrophages Cytokine Response to a Secondary Bacterial Trigger |
| title_short | HRV16 Impairs Macrophages Cytokine Response to a Secondary Bacterial Trigger |
| title_sort | hrv16 impairs macrophages cytokine response to a secondary bacterial trigger |
| topic | macrophage rhinovirus phagocytosis cytokine bacteria |
| url | https://www.frontiersin.org/article/10.3389/fimmu.2018.02908/full |
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