Spectroscopic and Theoretical Analysis of the Interaction between Plasma Proteins and Phthalimide Analogs with Potential Medical Application
One of the groups of organic compounds with potential use in medicine and pharmacy is phthalimide derivatives. They are characterized by a wide range of properties such as antibacterial, antifungal, and anti-inflammatory. In this study, we focused on research on four phthalimide derivatives with pro...
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| Format: | Article |
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MDPI AG
2023-03-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/13/3/760 |
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| author | Edward Krzyżak Aleksandra Kotynia Dominika Szkatuła Aleksandra Marciniak |
| author_facet | Edward Krzyżak Aleksandra Kotynia Dominika Szkatuła Aleksandra Marciniak |
| author_sort | Edward Krzyżak |
| collection | DOAJ |
| description | One of the groups of organic compounds with potential use in medicine and pharmacy is phthalimide derivatives. They are characterized by a wide range of properties such as antibacterial, antifungal, and anti-inflammatory. In this study, we focused on research on four phthalimide derivatives with proven non-toxicity, which are cyclooxygenase inhibitors. With the use of molecular docking study and spectroscopic methods, such as fluorescence, circular dichroism, and FT-IR spectroscopies, we analyzed the way the tested compounds interact with plasma proteins. Among the many proteins present in the plasma, we selected three: albumin, α1-acid glycoprotein, and gamma globulin, which play significant roles in the human body. The obtained results showed that all tested compounds bind to the analyzed proteins. They interact most strongly with albumin, which is a transport protein. However, interactions with serum albumin and orosomucoid do not cause significant changes in their structures. Only in the case of gamma globulins significant changes were observed in protein secondary structure. |
| first_indexed | 2024-03-11T06:17:43Z |
| format | Article |
| id | doaj.art-df79f42437e548a1bea5e55014af37c4 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-11T06:17:43Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-df79f42437e548a1bea5e55014af37c42023-11-17T12:11:59ZengMDPI AGLife2075-17292023-03-0113376010.3390/life13030760Spectroscopic and Theoretical Analysis of the Interaction between Plasma Proteins and Phthalimide Analogs with Potential Medical ApplicationEdward Krzyżak0Aleksandra Kotynia1Dominika Szkatuła2Aleksandra Marciniak3Department of Basic Chemical Sciences, Faculty of Pharmacy, Wroclaw Medical University, Borowska 211a, 50-556 Wrocław, PolandDepartment of Basic Chemical Sciences, Faculty of Pharmacy, Wroclaw Medical University, Borowska 211a, 50-556 Wrocław, PolandDepartment of Medicinal Chemistry, Faculty of Pharmacy, Wroclaw Medical University, Borowska 211, 50-556 Wrocław, PolandDepartment of Basic Chemical Sciences, Faculty of Pharmacy, Wroclaw Medical University, Borowska 211a, 50-556 Wrocław, PolandOne of the groups of organic compounds with potential use in medicine and pharmacy is phthalimide derivatives. They are characterized by a wide range of properties such as antibacterial, antifungal, and anti-inflammatory. In this study, we focused on research on four phthalimide derivatives with proven non-toxicity, which are cyclooxygenase inhibitors. With the use of molecular docking study and spectroscopic methods, such as fluorescence, circular dichroism, and FT-IR spectroscopies, we analyzed the way the tested compounds interact with plasma proteins. Among the many proteins present in the plasma, we selected three: albumin, α1-acid glycoprotein, and gamma globulin, which play significant roles in the human body. The obtained results showed that all tested compounds bind to the analyzed proteins. They interact most strongly with albumin, which is a transport protein. However, interactions with serum albumin and orosomucoid do not cause significant changes in their structures. Only in the case of gamma globulins significant changes were observed in protein secondary structure.https://www.mdpi.com/2075-1729/13/3/760fluorescence spectroscopycircular dichroism spectroscopyIR spectroscopyplasma proteinsphthalimide derivatives |
| spellingShingle | Edward Krzyżak Aleksandra Kotynia Dominika Szkatuła Aleksandra Marciniak Spectroscopic and Theoretical Analysis of the Interaction between Plasma Proteins and Phthalimide Analogs with Potential Medical Application Life fluorescence spectroscopy circular dichroism spectroscopy IR spectroscopy plasma proteins phthalimide derivatives |
| title | Spectroscopic and Theoretical Analysis of the Interaction between Plasma Proteins and Phthalimide Analogs with Potential Medical Application |
| title_full | Spectroscopic and Theoretical Analysis of the Interaction between Plasma Proteins and Phthalimide Analogs with Potential Medical Application |
| title_fullStr | Spectroscopic and Theoretical Analysis of the Interaction between Plasma Proteins and Phthalimide Analogs with Potential Medical Application |
| title_full_unstemmed | Spectroscopic and Theoretical Analysis of the Interaction between Plasma Proteins and Phthalimide Analogs with Potential Medical Application |
| title_short | Spectroscopic and Theoretical Analysis of the Interaction between Plasma Proteins and Phthalimide Analogs with Potential Medical Application |
| title_sort | spectroscopic and theoretical analysis of the interaction between plasma proteins and phthalimide analogs with potential medical application |
| topic | fluorescence spectroscopy circular dichroism spectroscopy IR spectroscopy plasma proteins phthalimide derivatives |
| url | https://www.mdpi.com/2075-1729/13/3/760 |
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