Multivariate optimization of Staphylococcus xylosus AD1 biomass production using sugarcane molasses plus yeast extract and soybean meal
Staphylococcus xylosus is a microorganism that has important physiological and technological characteristics that make it suitable for use as a starter culture in fermented meat products. For the development of these products in the food industry, it is necessary to produce biomass by the multiplica...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Universidade Estadual de Maringá
2019-12-01
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| Series: | Acta Scientiarum: Biological Sciences |
| Subjects: | |
| Online Access: | http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/47487 |
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| author | Leidi Daiana Preichardt Louise Haubert Maristela Cortez Sawitzki Teresinha Marisa Bertol Raul Vicenzi Adriana Dillenburg Meinhart Wladimir Padilha da Silva Ângela Maria Fiorentini |
| author_facet | Leidi Daiana Preichardt Louise Haubert Maristela Cortez Sawitzki Teresinha Marisa Bertol Raul Vicenzi Adriana Dillenburg Meinhart Wladimir Padilha da Silva Ângela Maria Fiorentini |
| author_sort | Leidi Daiana Preichardt |
| collection | DOAJ |
| description | Staphylococcus xylosus is a microorganism that has important physiological and technological characteristics that make it suitable for use as a starter culture in fermented meat products. For the development of these products in the food industry, it is necessary to produce biomass by the multiplication of starter cultures using low-cost media. This study developed a culture medium based on sugarcane molasses (SCM) supplemented with yeast extract (YE) and soybean meal (SM) to produce S. xylosus AD1 biomass employing a Box Behnken multivariate optimization design, using the best concentrations of the constituents of the culture medium for S. xylosus AD1 growth. By combining the mathematical models by the desirability function, it was possible to establish the optimal condition for the maximum production of viable cells and biomass. The optimal experimental condition was found when the fermentative process medium was composed of 10% SCM, 2% YE and 4% SM. In addition, the results of all experiments, except for the medium formulated with only SCM, presented a better performance than the commercial medium Brain Heart Infusion for the growth of S. xylosus AD1. The culture medium with agro-industrial byproduct (SCM) supplemented with YE and SM is an excellent alternative for producing S. xylosus AD1 biomass. |
| first_indexed | 2024-12-12T12:01:08Z |
| format | Article |
| id | doaj.art-df7f4b7d40d84298a1386f0b1eb45a0e |
| institution | Directory Open Access Journal |
| issn | 1679-9283 1807-863X |
| language | English |
| last_indexed | 2024-12-12T12:01:08Z |
| publishDate | 2019-12-01 |
| publisher | Universidade Estadual de Maringá |
| record_format | Article |
| series | Acta Scientiarum: Biological Sciences |
| spelling | doaj.art-df7f4b7d40d84298a1386f0b1eb45a0e2022-12-22T00:25:06ZengUniversidade Estadual de MaringáActa Scientiarum: Biological Sciences1679-92831807-863X2019-12-0141e47487e4748710.4025/actascibiolsci.v41i1.4748747487Multivariate optimization of Staphylococcus xylosus AD1 biomass production using sugarcane molasses plus yeast extract and soybean mealLeidi Daiana Preichardt0Louise Haubert1Maristela Cortez Sawitzki2Teresinha Marisa Bertol3Raul Vicenzi4Adriana Dillenburg Meinhart5Wladimir Padilha da Silva6Ângela Maria Fiorentini7Universidade Federal de Pelotas / Instituto Federal FarroupilhaUniversidade Federal de PelotasUniversidade Federal do PampaCentro Nacional de Pesquisa de Suínos e AvesUniversidade Regional do Noroeste do Estado do Rio Grande do SulUniversidade Federal de PelotasUniversidade Federal de PelotasUniversidade Federal de PelotasStaphylococcus xylosus is a microorganism that has important physiological and technological characteristics that make it suitable for use as a starter culture in fermented meat products. For the development of these products in the food industry, it is necessary to produce biomass by the multiplication of starter cultures using low-cost media. This study developed a culture medium based on sugarcane molasses (SCM) supplemented with yeast extract (YE) and soybean meal (SM) to produce S. xylosus AD1 biomass employing a Box Behnken multivariate optimization design, using the best concentrations of the constituents of the culture medium for S. xylosus AD1 growth. By combining the mathematical models by the desirability function, it was possible to establish the optimal condition for the maximum production of viable cells and biomass. The optimal experimental condition was found when the fermentative process medium was composed of 10% SCM, 2% YE and 4% SM. In addition, the results of all experiments, except for the medium formulated with only SCM, presented a better performance than the commercial medium Brain Heart Infusion for the growth of S. xylosus AD1. The culture medium with agro-industrial byproduct (SCM) supplemented with YE and SM is an excellent alternative for producing S. xylosus AD1 biomass.http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/47487agro-industrial by products; microbial production; starter culture. |
| spellingShingle | Leidi Daiana Preichardt Louise Haubert Maristela Cortez Sawitzki Teresinha Marisa Bertol Raul Vicenzi Adriana Dillenburg Meinhart Wladimir Padilha da Silva Ângela Maria Fiorentini Multivariate optimization of Staphylococcus xylosus AD1 biomass production using sugarcane molasses plus yeast extract and soybean meal Acta Scientiarum: Biological Sciences agro-industrial by products; microbial production; starter culture. |
| title | Multivariate optimization of Staphylococcus xylosus AD1 biomass production using sugarcane molasses plus yeast extract and soybean meal |
| title_full | Multivariate optimization of Staphylococcus xylosus AD1 biomass production using sugarcane molasses plus yeast extract and soybean meal |
| title_fullStr | Multivariate optimization of Staphylococcus xylosus AD1 biomass production using sugarcane molasses plus yeast extract and soybean meal |
| title_full_unstemmed | Multivariate optimization of Staphylococcus xylosus AD1 biomass production using sugarcane molasses plus yeast extract and soybean meal |
| title_short | Multivariate optimization of Staphylococcus xylosus AD1 biomass production using sugarcane molasses plus yeast extract and soybean meal |
| title_sort | multivariate optimization of staphylococcus xylosus ad1 biomass production using sugarcane molasses plus yeast extract and soybean meal |
| topic | agro-industrial by products; microbial production; starter culture. |
| url | http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/47487 |
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