Anthelmintic Properties of Essential Oils to Control Gastrointestinal Nematodes in Sheep—In Vitro and In Vivo Studies
Herbal products such as essential oils may play a promising role in the treatment of infections caused by gastrointestinal nematodes (GINs). The aim of this study was to evaluate the in vitro potential of 11 essential oils (EOs) and one binary combination of isolated EO compounds, as well as the in...
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MDPI AG
2022-02-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/9/2/93 |
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| author | Filip Štrbac Antonio Bosco Maria Paola Maurelli Radomir Ratajac Dragica Stojanović Nataša Simin Dejan Orčić Ivan Pušić Slobodan Krnjajić Smaragda Sotiraki Giorgio Saralli Giuseppe Cringoli Laura Rinaldi |
| author_facet | Filip Štrbac Antonio Bosco Maria Paola Maurelli Radomir Ratajac Dragica Stojanović Nataša Simin Dejan Orčić Ivan Pušić Slobodan Krnjajić Smaragda Sotiraki Giorgio Saralli Giuseppe Cringoli Laura Rinaldi |
| author_sort | Filip Štrbac |
| collection | DOAJ |
| description | Herbal products such as essential oils may play a promising role in the treatment of infections caused by gastrointestinal nematodes (GINs). The aim of this study was to evaluate the in vitro potential of 11 essential oils (EOs) and one binary combination of isolated EO compounds, as well as the in vivo anthelmintic efficacy of two EO formulations. Four GIN genera were identified in the coproculture examination: <i>Haemonchus</i>, <i>Trichostrongylus</i>, <i>Teladorsagia</i> and <i>Chabertia</i>. The in vitro egg hatch test (EHT) was performed at six different concentrations (50, 12.5, 3.125, 0.781, 0.195 and 0.049 mg/mL) for each EO, whereas in the in vivo faecal egg count reduction test (FECRT), each EO sample was diluted in sunflower oil and orally administrated at a dose of 100 mg/kg to the different group of animals. In the EHT, the EOs of <i>Origanum vulgare</i>, <i>Foeniculum vulgare</i>, <i>Satureja montana</i>, <i>Satureja hortensis</i> and two types of <i>Thymus vulgaris</i> were the most effective. The dominant compounds of these EOs were carvacrol, thymol, anethol, p-cymene and γ-terpinene, indicating their importance for the anthelmintic activity. In the FECRT, both <i>T. vulgaris</i> EO type 1 and linalool:estragole combination show an anthelmintic potential with a mean effect on FECR of approximately 25%. The results suggest the possible role of tested EOs as anthelmintic agents in sheep farms, although further in vivo tests are needed. |
| first_indexed | 2024-03-09T20:53:58Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-09T20:53:58Z |
| publishDate | 2022-02-01 |
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| spelling | doaj.art-df80283dac4f458b95131bdacb373f582023-11-23T22:28:33ZengMDPI AGVeterinary Sciences2306-73812022-02-01929310.3390/vetsci9020093Anthelmintic Properties of Essential Oils to Control Gastrointestinal Nematodes in Sheep—In Vitro and In Vivo StudiesFilip Štrbac0Antonio Bosco1Maria Paola Maurelli2Radomir Ratajac3Dragica Stojanović4Nataša Simin5Dejan Orčić6Ivan Pušić7Slobodan Krnjajić8Smaragda Sotiraki9Giorgio Saralli10Giuseppe Cringoli11Laura Rinaldi12Department of Veterinary Medicine, Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21102 Novi Sad, SerbiaDepartment of Veterinary Medicine and Animal Production, University of Naples Federico II, CREMOPAR, Via Federico Delpino 1, 80137 Naples, ItalyDepartment of Veterinary Medicine and Animal Production, University of Naples Federico II, CREMOPAR, Via Federico Delpino 1, 80137 Naples, ItalyScientific Veterinary Institute Novi Sad, Rumenački put 20, 21113 Novi Sad, SerbiaDepartment of Veterinary Medicine, Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21102 Novi Sad, SerbiaDepartment of Chemistry, Biochemistry and Environmental Protection, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovića 3, 21102 Novi Sad, SerbiaDepartment of Chemistry, Biochemistry and Environmental Protection, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovića 3, 21102 Novi Sad, SerbiaScientific Veterinary Institute Novi Sad, Rumenački put 20, 21113 Novi Sad, SerbiaInstitute for Multidisciplinary Research, University of Belgrade, Kneza Višeslava 1, 11030 Belgrade, SerbiaVeterinary Research Institute, National Agricultural Research Foundation, NAGREF Campus, 57001 Thessaloniki, GreeceExperimental Zooprophylactic Institute of Lazio and Tuscany M. Aleandri, Via Appia Nuova, 00178 Rome, ItalyDepartment of Veterinary Medicine and Animal Production, University of Naples Federico II, CREMOPAR, Via Federico Delpino 1, 80137 Naples, ItalyDepartment of Veterinary Medicine and Animal Production, University of Naples Federico II, CREMOPAR, Via Federico Delpino 1, 80137 Naples, ItalyHerbal products such as essential oils may play a promising role in the treatment of infections caused by gastrointestinal nematodes (GINs). The aim of this study was to evaluate the in vitro potential of 11 essential oils (EOs) and one binary combination of isolated EO compounds, as well as the in vivo anthelmintic efficacy of two EO formulations. Four GIN genera were identified in the coproculture examination: <i>Haemonchus</i>, <i>Trichostrongylus</i>, <i>Teladorsagia</i> and <i>Chabertia</i>. The in vitro egg hatch test (EHT) was performed at six different concentrations (50, 12.5, 3.125, 0.781, 0.195 and 0.049 mg/mL) for each EO, whereas in the in vivo faecal egg count reduction test (FECRT), each EO sample was diluted in sunflower oil and orally administrated at a dose of 100 mg/kg to the different group of animals. In the EHT, the EOs of <i>Origanum vulgare</i>, <i>Foeniculum vulgare</i>, <i>Satureja montana</i>, <i>Satureja hortensis</i> and two types of <i>Thymus vulgaris</i> were the most effective. The dominant compounds of these EOs were carvacrol, thymol, anethol, p-cymene and γ-terpinene, indicating their importance for the anthelmintic activity. In the FECRT, both <i>T. vulgaris</i> EO type 1 and linalool:estragole combination show an anthelmintic potential with a mean effect on FECR of approximately 25%. The results suggest the possible role of tested EOs as anthelmintic agents in sheep farms, although further in vivo tests are needed.https://www.mdpi.com/2306-7381/9/2/93phytotherapyessential oilsanthelmintic efficacygastrointestinal nematodesin vitro testin vivo test |
| spellingShingle | Filip Štrbac Antonio Bosco Maria Paola Maurelli Radomir Ratajac Dragica Stojanović Nataša Simin Dejan Orčić Ivan Pušić Slobodan Krnjajić Smaragda Sotiraki Giorgio Saralli Giuseppe Cringoli Laura Rinaldi Anthelmintic Properties of Essential Oils to Control Gastrointestinal Nematodes in Sheep—In Vitro and In Vivo Studies Veterinary Sciences phytotherapy essential oils anthelmintic efficacy gastrointestinal nematodes in vitro test in vivo test |
| title | Anthelmintic Properties of Essential Oils to Control Gastrointestinal Nematodes in Sheep—In Vitro and In Vivo Studies |
| title_full | Anthelmintic Properties of Essential Oils to Control Gastrointestinal Nematodes in Sheep—In Vitro and In Vivo Studies |
| title_fullStr | Anthelmintic Properties of Essential Oils to Control Gastrointestinal Nematodes in Sheep—In Vitro and In Vivo Studies |
| title_full_unstemmed | Anthelmintic Properties of Essential Oils to Control Gastrointestinal Nematodes in Sheep—In Vitro and In Vivo Studies |
| title_short | Anthelmintic Properties of Essential Oils to Control Gastrointestinal Nematodes in Sheep—In Vitro and In Vivo Studies |
| title_sort | anthelmintic properties of essential oils to control gastrointestinal nematodes in sheep in vitro and in vivo studies |
| topic | phytotherapy essential oils anthelmintic efficacy gastrointestinal nematodes in vitro test in vivo test |
| url | https://www.mdpi.com/2306-7381/9/2/93 |
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