ESI-MS/MS Identification of a Bradykinin-Potentiating Peptide from Amazon Bothrops atrox Snake Venom Using a Hybrid Qq-oaTOF Mass Spectrometer
A bradykinin-potentiating peptide (BPP) from Amazon Bothrops atrox venom with m/z 1384.7386 was identified and characterized by collision induced dissociation (CID) using an ESI-MS/MS spectra obtained in positive ion mode on a hybrid Qq-oaTOF mass spectrometer, Xevo G2 QTof MS (Waters, Manchester, U...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2013-02-01
|
| Series: | Toxins |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2072-6651/5/2/327 |
| _version_ | 1811306036964884480 |
|---|---|
| author | Leonardo A. Calderon Rodrigo G. Stábeli Juliana P. Zuliani Andreimar M. Soares Anderson M. Kayano Rodrigo S. Silva Kayena D. Zaqueo Gustavo H. M. F. Souza Cleópatra A. S. Caldeira Antonio Coutinho-Neto |
| author_facet | Leonardo A. Calderon Rodrigo G. Stábeli Juliana P. Zuliani Andreimar M. Soares Anderson M. Kayano Rodrigo S. Silva Kayena D. Zaqueo Gustavo H. M. F. Souza Cleópatra A. S. Caldeira Antonio Coutinho-Neto |
| author_sort | Leonardo A. Calderon |
| collection | DOAJ |
| description | A bradykinin-potentiating peptide (BPP) from Amazon Bothrops atrox venom with m/z 1384.7386 was identified and characterized by collision induced dissociation (CID) using an ESI-MS/MS spectra obtained in positive ion mode on a hybrid Qq-oaTOF mass spectrometer, Xevo G2 QTof MS (Waters, Manchester, UK). De novo peptide sequence analysis of the CID fragmentation spectra showed the amino acid sequence ZKWPRPGPEIPP, with a pyroglutamic acid and theoretical monoisotopic m/z 1384.7378, which is similar to experimental data, showing a mass accuracy of 0.6 ppm. The peptide is homologous to other BPP from Bothrops moojeni and was named as BPP-BAX12. |
| first_indexed | 2024-04-13T08:37:09Z |
| format | Article |
| id | doaj.art-df809794b3ce4556871a64bde6922eb2 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-13T08:37:09Z |
| publishDate | 2013-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-df809794b3ce4556871a64bde6922eb22022-12-22T02:54:03ZengMDPI AGToxins2072-66512013-02-015232733510.3390/toxins5020327ESI-MS/MS Identification of a Bradykinin-Potentiating Peptide from Amazon Bothrops atrox Snake Venom Using a Hybrid Qq-oaTOF Mass SpectrometerLeonardo A. CalderonRodrigo G. StábeliJuliana P. ZulianiAndreimar M. SoaresAnderson M. KayanoRodrigo S. SilvaKayena D. ZaqueoGustavo H. M. F. SouzaCleópatra A. S. CaldeiraAntonio Coutinho-NetoA bradykinin-potentiating peptide (BPP) from Amazon Bothrops atrox venom with m/z 1384.7386 was identified and characterized by collision induced dissociation (CID) using an ESI-MS/MS spectra obtained in positive ion mode on a hybrid Qq-oaTOF mass spectrometer, Xevo G2 QTof MS (Waters, Manchester, UK). De novo peptide sequence analysis of the CID fragmentation spectra showed the amino acid sequence ZKWPRPGPEIPP, with a pyroglutamic acid and theoretical monoisotopic m/z 1384.7378, which is similar to experimental data, showing a mass accuracy of 0.6 ppm. The peptide is homologous to other BPP from Bothrops moojeni and was named as BPP-BAX12.http://www.mdpi.com/2072-6651/5/2/327bioactive peptideBPPpyroglutamic acidpyrrolidonecarboxylic acidde novo peptide sequencing |
| spellingShingle | Leonardo A. Calderon Rodrigo G. Stábeli Juliana P. Zuliani Andreimar M. Soares Anderson M. Kayano Rodrigo S. Silva Kayena D. Zaqueo Gustavo H. M. F. Souza Cleópatra A. S. Caldeira Antonio Coutinho-Neto ESI-MS/MS Identification of a Bradykinin-Potentiating Peptide from Amazon Bothrops atrox Snake Venom Using a Hybrid Qq-oaTOF Mass Spectrometer Toxins bioactive peptide BPP pyroglutamic acid pyrrolidonecarboxylic acid de novo peptide sequencing |
| title | ESI-MS/MS Identification of a Bradykinin-Potentiating Peptide from Amazon Bothrops atrox Snake Venom Using a Hybrid Qq-oaTOF Mass Spectrometer |
| title_full | ESI-MS/MS Identification of a Bradykinin-Potentiating Peptide from Amazon Bothrops atrox Snake Venom Using a Hybrid Qq-oaTOF Mass Spectrometer |
| title_fullStr | ESI-MS/MS Identification of a Bradykinin-Potentiating Peptide from Amazon Bothrops atrox Snake Venom Using a Hybrid Qq-oaTOF Mass Spectrometer |
| title_full_unstemmed | ESI-MS/MS Identification of a Bradykinin-Potentiating Peptide from Amazon Bothrops atrox Snake Venom Using a Hybrid Qq-oaTOF Mass Spectrometer |
| title_short | ESI-MS/MS Identification of a Bradykinin-Potentiating Peptide from Amazon Bothrops atrox Snake Venom Using a Hybrid Qq-oaTOF Mass Spectrometer |
| title_sort | esi ms ms identification of a bradykinin potentiating peptide from amazon bothrops atrox snake venom using a hybrid qq oatof mass spectrometer |
| topic | bioactive peptide BPP pyroglutamic acid pyrrolidonecarboxylic acid de novo peptide sequencing |
| url | http://www.mdpi.com/2072-6651/5/2/327 |
| work_keys_str_mv | AT leonardoacalderon esimsmsidentificationofabradykininpotentiatingpeptidefromamazonbothropsatroxsnakevenomusingahybridqqoatofmassspectrometer AT rodrigogstabeli esimsmsidentificationofabradykininpotentiatingpeptidefromamazonbothropsatroxsnakevenomusingahybridqqoatofmassspectrometer AT julianapzuliani esimsmsidentificationofabradykininpotentiatingpeptidefromamazonbothropsatroxsnakevenomusingahybridqqoatofmassspectrometer AT andreimarmsoares esimsmsidentificationofabradykininpotentiatingpeptidefromamazonbothropsatroxsnakevenomusingahybridqqoatofmassspectrometer AT andersonmkayano esimsmsidentificationofabradykininpotentiatingpeptidefromamazonbothropsatroxsnakevenomusingahybridqqoatofmassspectrometer AT rodrigossilva esimsmsidentificationofabradykininpotentiatingpeptidefromamazonbothropsatroxsnakevenomusingahybridqqoatofmassspectrometer AT kayenadzaqueo esimsmsidentificationofabradykininpotentiatingpeptidefromamazonbothropsatroxsnakevenomusingahybridqqoatofmassspectrometer AT gustavohmfsouza esimsmsidentificationofabradykininpotentiatingpeptidefromamazonbothropsatroxsnakevenomusingahybridqqoatofmassspectrometer AT cleopatraascaldeira esimsmsidentificationofabradykininpotentiatingpeptidefromamazonbothropsatroxsnakevenomusingahybridqqoatofmassspectrometer AT antoniocoutinhoneto esimsmsidentificationofabradykininpotentiatingpeptidefromamazonbothropsatroxsnakevenomusingahybridqqoatofmassspectrometer |