Biotechnological Production of Fumaric Acid: The Effect of Morphology of Rhizopus arrhizus NRRL 2582
Fumaric acid is a platform chemical with many applications in bio-based chemical and polymer production. Fungal cell morphology is an important factor that affects fumaric acid production via fermentation. In the present study, pellet and dispersed mycelia morphology of Rhizopus arrhizus NRRL 2582 w...
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-07-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/3/3/33 |
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| author | Aikaterini Papadaki Nikolaos Androutsopoulos Maria Patsalou Michalis Koutinas Nikolaos Kopsahelis Aline Machado de Castro Seraphim Papanikolaou Apostolis A. Koutinas |
| author_facet | Aikaterini Papadaki Nikolaos Androutsopoulos Maria Patsalou Michalis Koutinas Nikolaos Kopsahelis Aline Machado de Castro Seraphim Papanikolaou Apostolis A. Koutinas |
| author_sort | Aikaterini Papadaki |
| collection | DOAJ |
| description | Fumaric acid is a platform chemical with many applications in bio-based chemical and polymer production. Fungal cell morphology is an important factor that affects fumaric acid production via fermentation. In the present study, pellet and dispersed mycelia morphology of Rhizopus arrhizus NRRL 2582 was analysed using image analysis software and the impact on fumaric acid production was evaluated. Batch experiments were carried out in shake flasks using glucose as carbon source. The highest fumaric acid yield of 0.84 g/g total sugars was achieved in the case of dispersed mycelia with a final fumaric acid concentration of 19.7 g/L. The fumaric acid production was also evaluated using a nutrient rich feedstock obtained from soybean cake, as substitute of the commercial nitrogen sources. Solid state fermentation was performed in order to produce proteolytic enzymes, which were utilised for soybean cake hydrolysis. Batch fermentations were conducted using 50 g/L glucose and soybean cake hydrolysate achieving up to 33 g/L fumaric acid concentration. To the best of our knowledge the influence of R. arrhizus morphology on fumaric acid production has not been reported previously. The results indicated that dispersed clumps were more effective in fumaric acid production than pellets and renewable resources could be alternatively valorised for the biotechnological production of platform chemicals. |
| first_indexed | 2024-12-20T20:53:58Z |
| format | Article |
| id | doaj.art-df8107e99a3746d0804e6e6123fec32e |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-12-20T20:53:58Z |
| publishDate | 2017-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-df8107e99a3746d0804e6e6123fec32e2022-12-21T19:26:52ZengMDPI AGFermentation2311-56372017-07-01333310.3390/fermentation3030033fermentation3030033Biotechnological Production of Fumaric Acid: The Effect of Morphology of Rhizopus arrhizus NRRL 2582Aikaterini Papadaki0Nikolaos Androutsopoulos1Maria Patsalou2Michalis Koutinas3Nikolaos Kopsahelis4Aline Machado de Castro5Seraphim Papanikolaou6Apostolis A. Koutinas7Department of Food Science and Human Nutrition, Agricultural University of Athens, Iera Odos 75, 11855 Athens, GreeceDepartment of Food Science and Human Nutrition, Agricultural University of Athens, Iera Odos 75, 11855 Athens, GreeceDepartment of Environmental Science & Technology, Cyprus University of Technology, 30 Archbishop Kyprianou Str., 3036 Limassol, CyprusDepartment of Environmental Science & Technology, Cyprus University of Technology, 30 Archbishop Kyprianou Str., 3036 Limassol, CyprusDepartment of Food Science and Human Nutrition, Agricultural University of Athens, Iera Odos 75, 11855 Athens, GreeceBiotechnology Division, Research and Development Centre, PETROBRAS, Rio de Janeiro 20000-000, BrazilDepartment of Food Science and Human Nutrition, Agricultural University of Athens, Iera Odos 75, 11855 Athens, GreeceDepartment of Food Science and Human Nutrition, Agricultural University of Athens, Iera Odos 75, 11855 Athens, GreeceFumaric acid is a platform chemical with many applications in bio-based chemical and polymer production. Fungal cell morphology is an important factor that affects fumaric acid production via fermentation. In the present study, pellet and dispersed mycelia morphology of Rhizopus arrhizus NRRL 2582 was analysed using image analysis software and the impact on fumaric acid production was evaluated. Batch experiments were carried out in shake flasks using glucose as carbon source. The highest fumaric acid yield of 0.84 g/g total sugars was achieved in the case of dispersed mycelia with a final fumaric acid concentration of 19.7 g/L. The fumaric acid production was also evaluated using a nutrient rich feedstock obtained from soybean cake, as substitute of the commercial nitrogen sources. Solid state fermentation was performed in order to produce proteolytic enzymes, which were utilised for soybean cake hydrolysis. Batch fermentations were conducted using 50 g/L glucose and soybean cake hydrolysate achieving up to 33 g/L fumaric acid concentration. To the best of our knowledge the influence of R. arrhizus morphology on fumaric acid production has not been reported previously. The results indicated that dispersed clumps were more effective in fumaric acid production than pellets and renewable resources could be alternatively valorised for the biotechnological production of platform chemicals.https://www.mdpi.com/2311-5637/3/3/33fumaric acidRhizopusfungal morphologysoybean cakeenzymatic hydrolysisAspergillus oryzaebiorefineryimage analysis |
| spellingShingle | Aikaterini Papadaki Nikolaos Androutsopoulos Maria Patsalou Michalis Koutinas Nikolaos Kopsahelis Aline Machado de Castro Seraphim Papanikolaou Apostolis A. Koutinas Biotechnological Production of Fumaric Acid: The Effect of Morphology of Rhizopus arrhizus NRRL 2582 Fermentation fumaric acid Rhizopus fungal morphology soybean cake enzymatic hydrolysis Aspergillus oryzae biorefinery image analysis |
| title | Biotechnological Production of Fumaric Acid: The Effect of Morphology of Rhizopus arrhizus NRRL 2582 |
| title_full | Biotechnological Production of Fumaric Acid: The Effect of Morphology of Rhizopus arrhizus NRRL 2582 |
| title_fullStr | Biotechnological Production of Fumaric Acid: The Effect of Morphology of Rhizopus arrhizus NRRL 2582 |
| title_full_unstemmed | Biotechnological Production of Fumaric Acid: The Effect of Morphology of Rhizopus arrhizus NRRL 2582 |
| title_short | Biotechnological Production of Fumaric Acid: The Effect of Morphology of Rhizopus arrhizus NRRL 2582 |
| title_sort | biotechnological production of fumaric acid the effect of morphology of rhizopus arrhizus nrrl 2582 |
| topic | fumaric acid Rhizopus fungal morphology soybean cake enzymatic hydrolysis Aspergillus oryzae biorefinery image analysis |
| url | https://www.mdpi.com/2311-5637/3/3/33 |
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