Prediction of buffalo bull fertility on the basis of sperm motion traits, viability, membrane integrity, heat shock protein (HSP70) expression and fertility associated antigen (FAA)
The present study was conducted on 20 buffalo bulls to predict the fertility on the basis of: (a) Computer assisted semen analysis (CASA) based sperm motion traits, (b) viability, (c) membrane integrity, (d) expression for HSP70, and (e) assessment of fertility associated antigen (FAA). Six frozen...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Indian Council of Agricultural Research
2016-06-01
|
| Series: | Indian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | https://epubs.icar.org.in/index.php/IJAnS/article/view/59160 |
| _version_ | 1797741441007484928 |
|---|---|
| author | AJEET KUMAR JAGIR SINGH G V P P S RAVI KUMAR RANJANA S CHEEMA A K PANDEY PAWAN SINGH S P S GHUMAN P S BRAR V K GANDOTRA |
| author_facet | AJEET KUMAR JAGIR SINGH G V P P S RAVI KUMAR RANJANA S CHEEMA A K PANDEY PAWAN SINGH S P S GHUMAN P S BRAR V K GANDOTRA |
| author_sort | AJEET KUMAR |
| collection | DOAJ |
| description |
The present study was conducted on 20 buffalo bulls to predict the fertility on the basis of: (a) Computer assisted semen analysis (CASA) based sperm motion traits, (b) viability, (c) membrane integrity, (d) expression for HSP70, and (e) assessment of fertility associated antigen (FAA). Six frozen semen straws from each buffalo bulls were analyzed for sperm motion traits, viz. individual motility, progressive motility, average path velocity (VAP), straight line velocity (VSL), curvilinear velocity (VCL), amplitude of lateral head deviation (ALH), beat cross frequency (BCF), straightness (STR), linearity (LIN) and sperm size. Viability and hypoosmotic swelling tests (HOST) were also conducted. Heat shock protein 70 (HSP70) expression and quantification was conducted using a real time PCR. Fertility associated antigen (FAA) was assessed in fresh semen from buffalo bulls using test. Fertility trial was conducted on 250 normal cycling buffaloes following estrus synchronization with GnRH-PGF- GnRH protocol. It was concluded that buffalo bull fertility could be best predicted on the basis of sperm motion traits (VCL, VAP, VSL, ALH, LIN), motility, viability, HOST and HSP70 expression. However, FAA assessment in fresh semen did not indicate fertility.
|
| first_indexed | 2024-03-12T14:26:45Z |
| format | Article |
| id | doaj.art-df821ea94388474a9189bac1dd4bf909 |
| institution | Directory Open Access Journal |
| issn | 0367-8318 2394-3327 |
| language | English |
| last_indexed | 2024-03-12T14:26:45Z |
| publishDate | 2016-06-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | Indian Journal of Animal Sciences |
| spelling | doaj.art-df821ea94388474a9189bac1dd4bf9092023-08-18T06:08:28ZengIndian Council of Agricultural ResearchIndian Journal of Animal Sciences0367-83182394-33272016-06-0186610.56093/ijans.v86i6.59160Prediction of buffalo bull fertility on the basis of sperm motion traits, viability, membrane integrity, heat shock protein (HSP70) expression and fertility associated antigen (FAA)AJEET KUMAR0JAGIR SINGH1G V P P S RAVI KUMAR2RANJANA S CHEEMA3A K PANDEY4PAWAN SINGH5S P S GHUMAN6P S BRAR7V K GANDOTRA8Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 IndiaGuru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 IndiaIVRI, BareillyGuru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 IndiaLLRVASU, HisarNDRI, KarnalGuru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 IndiaGuru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 IndiaGuru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India The present study was conducted on 20 buffalo bulls to predict the fertility on the basis of: (a) Computer assisted semen analysis (CASA) based sperm motion traits, (b) viability, (c) membrane integrity, (d) expression for HSP70, and (e) assessment of fertility associated antigen (FAA). Six frozen semen straws from each buffalo bulls were analyzed for sperm motion traits, viz. individual motility, progressive motility, average path velocity (VAP), straight line velocity (VSL), curvilinear velocity (VCL), amplitude of lateral head deviation (ALH), beat cross frequency (BCF), straightness (STR), linearity (LIN) and sperm size. Viability and hypoosmotic swelling tests (HOST) were also conducted. Heat shock protein 70 (HSP70) expression and quantification was conducted using a real time PCR. Fertility associated antigen (FAA) was assessed in fresh semen from buffalo bulls using test. Fertility trial was conducted on 250 normal cycling buffaloes following estrus synchronization with GnRH-PGF- GnRH protocol. It was concluded that buffalo bull fertility could be best predicted on the basis of sperm motion traits (VCL, VAP, VSL, ALH, LIN), motility, viability, HOST and HSP70 expression. However, FAA assessment in fresh semen did not indicate fertility. https://epubs.icar.org.in/index.php/IJAnS/article/view/59160Buffalo bullsFertility associated antigenFertility predictionHSP70Membrane integritySperm motion traits |
| spellingShingle | AJEET KUMAR JAGIR SINGH G V P P S RAVI KUMAR RANJANA S CHEEMA A K PANDEY PAWAN SINGH S P S GHUMAN P S BRAR V K GANDOTRA Prediction of buffalo bull fertility on the basis of sperm motion traits, viability, membrane integrity, heat shock protein (HSP70) expression and fertility associated antigen (FAA) Indian Journal of Animal Sciences Buffalo bulls Fertility associated antigen Fertility prediction HSP70 Membrane integrity Sperm motion traits |
| title | Prediction of buffalo bull fertility on the basis of sperm motion traits, viability, membrane integrity, heat shock protein (HSP70) expression and fertility associated antigen (FAA) |
| title_full | Prediction of buffalo bull fertility on the basis of sperm motion traits, viability, membrane integrity, heat shock protein (HSP70) expression and fertility associated antigen (FAA) |
| title_fullStr | Prediction of buffalo bull fertility on the basis of sperm motion traits, viability, membrane integrity, heat shock protein (HSP70) expression and fertility associated antigen (FAA) |
| title_full_unstemmed | Prediction of buffalo bull fertility on the basis of sperm motion traits, viability, membrane integrity, heat shock protein (HSP70) expression and fertility associated antigen (FAA) |
| title_short | Prediction of buffalo bull fertility on the basis of sperm motion traits, viability, membrane integrity, heat shock protein (HSP70) expression and fertility associated antigen (FAA) |
| title_sort | prediction of buffalo bull fertility on the basis of sperm motion traits viability membrane integrity heat shock protein hsp70 expression and fertility associated antigen faa |
| topic | Buffalo bulls Fertility associated antigen Fertility prediction HSP70 Membrane integrity Sperm motion traits |
| url | https://epubs.icar.org.in/index.php/IJAnS/article/view/59160 |
| work_keys_str_mv | AT ajeetkumar predictionofbuffalobullfertilityonthebasisofspermmotiontraitsviabilitymembraneintegrityheatshockproteinhsp70expressionandfertilityassociatedantigenfaa AT jagirsingh predictionofbuffalobullfertilityonthebasisofspermmotiontraitsviabilitymembraneintegrityheatshockproteinhsp70expressionandfertilityassociatedantigenfaa AT gvppsravikumar predictionofbuffalobullfertilityonthebasisofspermmotiontraitsviabilitymembraneintegrityheatshockproteinhsp70expressionandfertilityassociatedantigenfaa AT ranjanascheema predictionofbuffalobullfertilityonthebasisofspermmotiontraitsviabilitymembraneintegrityheatshockproteinhsp70expressionandfertilityassociatedantigenfaa AT akpandey predictionofbuffalobullfertilityonthebasisofspermmotiontraitsviabilitymembraneintegrityheatshockproteinhsp70expressionandfertilityassociatedantigenfaa AT pawansingh predictionofbuffalobullfertilityonthebasisofspermmotiontraitsviabilitymembraneintegrityheatshockproteinhsp70expressionandfertilityassociatedantigenfaa AT spsghuman predictionofbuffalobullfertilityonthebasisofspermmotiontraitsviabilitymembraneintegrityheatshockproteinhsp70expressionandfertilityassociatedantigenfaa AT psbrar predictionofbuffalobullfertilityonthebasisofspermmotiontraitsviabilitymembraneintegrityheatshockproteinhsp70expressionandfertilityassociatedantigenfaa AT vkgandotra predictionofbuffalobullfertilityonthebasisofspermmotiontraitsviabilitymembraneintegrityheatshockproteinhsp70expressionandfertilityassociatedantigenfaa |