Achilles tendon suture deteriorates tendon capillary blood flow with sustained tissue oxygen saturation – an animal study
<p>Abstract</p> <p>Background</p> <p>Treatment of ruptured Achilles tendons currently constitutes of conservative early functional treatment or surgical treatment either by open or minimal invasive techniques. We hypothesize that an experimental Achilles tendon suture i...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2009-08-01
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| Series: | Journal of Orthopaedic Surgery and Research |
| Online Access: | http://www.josr-online.com/content/4/1/32 |
| _version_ | 1828153369961693184 |
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| author | Vogt Peter M Rotter Robert Lorenzen Johan Kraemer Robert Knobloch Karsten |
| author_facet | Vogt Peter M Rotter Robert Lorenzen Johan Kraemer Robert Knobloch Karsten |
| author_sort | Vogt Peter M |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Treatment of ruptured Achilles tendons currently constitutes of conservative early functional treatment or surgical treatment either by open or minimal invasive techniques. We hypothesize that an experimental Achilles tendon suture in an animal model significantly deteriorates Achilles tendon microcirculation immediately following suturing.</p> <p>Methods</p> <p>Fifteen Achilles tendons of eight male Wistar rats (275–325 g) were included. After preparation of the Achilles tendon with a medial paratendinous approach, Achilles tendon microcirculation was assessed using combined Laser-Doppler and spectrophotometry (Oxygen-to-see) regarding:</p> <p indent="1">- tendinous capillary blood flow [arbitrary units AU]</p> <p indent="1">- tendinous tissue oxygen saturation [%]</p> <p indent="1">- tendinous venous filling pressure [rAU]</p> <p>The main body of the Achilles tendon was measured in the center of the suture with 50 Hz. 10 minutes after Achilles tendon suture (6-0 Prolene), a second assessment of microcirculatory parameters was performed.</p> <p>Results</p> <p>Achilles tendon capillary blood flow decreased by 57% following the suture (70 ± 30 AU vs. 31 ± 16 AU; p < 0.001). Tendinous tissue oxygen saturation remained at the same level before and after suture (78 ± 17% vs. 77 ± 22%; p = 0.904). Tendinous venous filling pressure increased by 33% (54 ± 16 AU vs. 72 ± 20 AU; p = 0.019) after suture.</p> <p>Conclusion</p> <p>Achilles tendon suture in anaesthetised rats causes an acute loss of capillary perfusion and increases postcapillary venous filling pressures indicating venous stasis. The primary hypothesis of this study was confirmed. In contrast, tendinous tissue oxygen saturation remains unchanged excluding acute intratendinous hypoxia within the first 10 minutes after suture. Further changes of oxygen saturation remain unclear. Furthermore, it remains to be determined to what extent reduced capillary blood flow as well as increased postcapillary stasis might influence tendon healing from a microcirculatory point of view in this animal setting.</p> |
| first_indexed | 2024-04-11T22:25:05Z |
| format | Article |
| id | doaj.art-df826c87e9a74f618d75562ff05a3609 |
| institution | Directory Open Access Journal |
| issn | 1749-799X |
| language | English |
| last_indexed | 2024-04-11T22:25:05Z |
| publishDate | 2009-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Orthopaedic Surgery and Research |
| spelling | doaj.art-df826c87e9a74f618d75562ff05a36092022-12-22T03:59:49ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2009-08-01413210.1186/1749-799X-4-32Achilles tendon suture deteriorates tendon capillary blood flow with sustained tissue oxygen saturation – an animal studyVogt Peter MRotter RobertLorenzen JohanKraemer RobertKnobloch Karsten<p>Abstract</p> <p>Background</p> <p>Treatment of ruptured Achilles tendons currently constitutes of conservative early functional treatment or surgical treatment either by open or minimal invasive techniques. We hypothesize that an experimental Achilles tendon suture in an animal model significantly deteriorates Achilles tendon microcirculation immediately following suturing.</p> <p>Methods</p> <p>Fifteen Achilles tendons of eight male Wistar rats (275–325 g) were included. After preparation of the Achilles tendon with a medial paratendinous approach, Achilles tendon microcirculation was assessed using combined Laser-Doppler and spectrophotometry (Oxygen-to-see) regarding:</p> <p indent="1">- tendinous capillary blood flow [arbitrary units AU]</p> <p indent="1">- tendinous tissue oxygen saturation [%]</p> <p indent="1">- tendinous venous filling pressure [rAU]</p> <p>The main body of the Achilles tendon was measured in the center of the suture with 50 Hz. 10 minutes after Achilles tendon suture (6-0 Prolene), a second assessment of microcirculatory parameters was performed.</p> <p>Results</p> <p>Achilles tendon capillary blood flow decreased by 57% following the suture (70 ± 30 AU vs. 31 ± 16 AU; p < 0.001). Tendinous tissue oxygen saturation remained at the same level before and after suture (78 ± 17% vs. 77 ± 22%; p = 0.904). Tendinous venous filling pressure increased by 33% (54 ± 16 AU vs. 72 ± 20 AU; p = 0.019) after suture.</p> <p>Conclusion</p> <p>Achilles tendon suture in anaesthetised rats causes an acute loss of capillary perfusion and increases postcapillary venous filling pressures indicating venous stasis. The primary hypothesis of this study was confirmed. In contrast, tendinous tissue oxygen saturation remains unchanged excluding acute intratendinous hypoxia within the first 10 minutes after suture. Further changes of oxygen saturation remain unclear. Furthermore, it remains to be determined to what extent reduced capillary blood flow as well as increased postcapillary stasis might influence tendon healing from a microcirculatory point of view in this animal setting.</p>http://www.josr-online.com/content/4/1/32 |
| spellingShingle | Vogt Peter M Rotter Robert Lorenzen Johan Kraemer Robert Knobloch Karsten Achilles tendon suture deteriorates tendon capillary blood flow with sustained tissue oxygen saturation – an animal study Journal of Orthopaedic Surgery and Research |
| title | Achilles tendon suture deteriorates tendon capillary blood flow with sustained tissue oxygen saturation – an animal study |
| title_full | Achilles tendon suture deteriorates tendon capillary blood flow with sustained tissue oxygen saturation – an animal study |
| title_fullStr | Achilles tendon suture deteriorates tendon capillary blood flow with sustained tissue oxygen saturation – an animal study |
| title_full_unstemmed | Achilles tendon suture deteriorates tendon capillary blood flow with sustained tissue oxygen saturation – an animal study |
| title_short | Achilles tendon suture deteriorates tendon capillary blood flow with sustained tissue oxygen saturation – an animal study |
| title_sort | achilles tendon suture deteriorates tendon capillary blood flow with sustained tissue oxygen saturation an animal study |
| url | http://www.josr-online.com/content/4/1/32 |
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