Chitosanases from Family 46 of Glycoside Hydrolases: From Proteins to Phenotypes
Chitosanases, enzymes that catalyze the endo-hydrolysis of glycolytic links in chitosan, are the subject of numerous studies as biotechnological tools to generate low molecular weight chitosan (LMWC) or chitosan oligosaccharides (CHOS) from native, high molecular weight chitosan. Glycoside hydrolas...
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| Format: | Article |
| Language: | English |
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MDPI AG
2015-10-01
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| Series: | Marine Drugs |
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| Online Access: | http://www.mdpi.com/1660-3397/13/11/6566 |
| _version_ | 1828234062822637568 |
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| author | Pascal Viens Marie-Ève Lacombe-Harvey Ryszard Brzezinski |
| author_facet | Pascal Viens Marie-Ève Lacombe-Harvey Ryszard Brzezinski |
| author_sort | Pascal Viens |
| collection | DOAJ |
| description | Chitosanases, enzymes that catalyze the endo-hydrolysis of glycolytic links in chitosan, are the subject of numerous studies as biotechnological tools to generate low molecular weight chitosan (LMWC) or chitosan oligosaccharides (CHOS) from native, high molecular weight chitosan. Glycoside hydrolases belonging to family GH46 are among the best-studied chitosanases, with four crystallography-derived structures available and more than forty enzymes studied at the biochemical level. They were also subjected to numerous site-directed mutagenesis studies, unraveling the molecular mechanisms of hydrolysis. This review is focused on the taxonomic distribution of GH46 proteins, their multi-modular character, the structure-function relationships and their biological functions in the host organisms. |
| first_indexed | 2024-04-12T19:49:13Z |
| format | Article |
| id | doaj.art-df82ac6a05f946428cc5e19685b0fb02 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-12T19:49:13Z |
| publishDate | 2015-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-df82ac6a05f946428cc5e19685b0fb022022-12-22T03:18:53ZengMDPI AGMarine Drugs1660-33972015-10-0113116566658710.3390/md13116566md13116566Chitosanases from Family 46 of Glycoside Hydrolases: From Proteins to PhenotypesPascal Viens0Marie-Ève Lacombe-Harvey1Ryszard Brzezinski2Biologie, Faculté des Sciences, Université de Sherbrooke, 2500, boul. de l’Université, Sherbrooke, QC J1K 2R1, CanadaBiologie, Faculté des Sciences, Université de Sherbrooke, 2500, boul. de l’Université, Sherbrooke, QC J1K 2R1, CanadaBiologie, Faculté des Sciences, Université de Sherbrooke, 2500, boul. de l’Université, Sherbrooke, QC J1K 2R1, CanadaChitosanases, enzymes that catalyze the endo-hydrolysis of glycolytic links in chitosan, are the subject of numerous studies as biotechnological tools to generate low molecular weight chitosan (LMWC) or chitosan oligosaccharides (CHOS) from native, high molecular weight chitosan. Glycoside hydrolases belonging to family GH46 are among the best-studied chitosanases, with four crystallography-derived structures available and more than forty enzymes studied at the biochemical level. They were also subjected to numerous site-directed mutagenesis studies, unraveling the molecular mechanisms of hydrolysis. This review is focused on the taxonomic distribution of GH46 proteins, their multi-modular character, the structure-function relationships and their biological functions in the host organisms.http://www.mdpi.com/1660-3397/13/11/6566chitosanchitosanaseglycoside hydrolaseStreptomycesBacillusMicrobacteriumhydrolysispolysaccharidechlorovirus |
| spellingShingle | Pascal Viens Marie-Ève Lacombe-Harvey Ryszard Brzezinski Chitosanases from Family 46 of Glycoside Hydrolases: From Proteins to Phenotypes Marine Drugs chitosan chitosanase glycoside hydrolase Streptomyces Bacillus Microbacterium hydrolysis polysaccharide chlorovirus |
| title | Chitosanases from Family 46 of Glycoside Hydrolases: From Proteins to Phenotypes |
| title_full | Chitosanases from Family 46 of Glycoside Hydrolases: From Proteins to Phenotypes |
| title_fullStr | Chitosanases from Family 46 of Glycoside Hydrolases: From Proteins to Phenotypes |
| title_full_unstemmed | Chitosanases from Family 46 of Glycoside Hydrolases: From Proteins to Phenotypes |
| title_short | Chitosanases from Family 46 of Glycoside Hydrolases: From Proteins to Phenotypes |
| title_sort | chitosanases from family 46 of glycoside hydrolases from proteins to phenotypes |
| topic | chitosan chitosanase glycoside hydrolase Streptomyces Bacillus Microbacterium hydrolysis polysaccharide chlorovirus |
| url | http://www.mdpi.com/1660-3397/13/11/6566 |
| work_keys_str_mv | AT pascalviens chitosanasesfromfamily46ofglycosidehydrolasesfromproteinstophenotypes AT marieevelacombeharvey chitosanasesfromfamily46ofglycosidehydrolasesfromproteinstophenotypes AT ryszardbrzezinski chitosanasesfromfamily46ofglycosidehydrolasesfromproteinstophenotypes |