An all-silk-derived bilayer hydrogel for osteochondral tissue engineering
Osteochondral repair remains a challenge in clinical practice nowadays despite extensive advances in tissue engineering. The insufficient recruitment of endogenous cells in the early stage and incomplete cell differentiation in the later stage constitute the major difficulty of osteochondral repair....
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006422002836 |
| _version_ | 1797984343147151360 |
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| author | Weizhou Jiang Xiuting Xiang Minkai Song Jianlin Shen Zhanjun Shi Wenhua Huang Huan Liu |
| author_facet | Weizhou Jiang Xiuting Xiang Minkai Song Jianlin Shen Zhanjun Shi Wenhua Huang Huan Liu |
| author_sort | Weizhou Jiang |
| collection | DOAJ |
| description | Osteochondral repair remains a challenge in clinical practice nowadays despite extensive advances in tissue engineering. The insufficient recruitment of endogenous cells in the early stage and incomplete cell differentiation in the later stage constitute the major difficulty of osteochondral repair. Here, a novel all-silk-derived multifunctional biomaterial platform for osteochondral engineering is reported. The bilayer methacrylated silk fibroin (SilMA) hydrogel was fabricated through stratified photocuring as the basic provisional matrix for tissue regeneration. Platelet-rich plasma (PRP) incorporation promoted the migration and pre-differentiation of the bone marrow mesenchymal stem cells (BMSCs) in the early stage of implantation. The long-term regulation of BMSCs chondrogenesis and osteogenesis was realized by the stratified anchoring of the silk fibroin (SF) microspheres respectively loaded with Kartogenin (KGN) and berberine (BBR) in the hydrogel. The composite hydrogels were further demonstrated to promote BMSCs chondrogenic and osteogenic differentiation under an inflammatory microenvironment and to achieve satisfying cartilage and subchondral bone regeneration with great biocompatibility after 8 weeks of implantation. Since all the components used are readily available and biocompatible and can be efficiently integrated via a simple process, this composite hydrogel scaffold has tremendous potential for clinical use in osteochondral regeneration. |
| first_indexed | 2024-04-11T07:00:35Z |
| format | Article |
| id | doaj.art-df836658fad34bfc9a5570336b30b35e |
| institution | Directory Open Access Journal |
| issn | 2590-0064 |
| language | English |
| last_indexed | 2024-04-11T07:00:35Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj.art-df836658fad34bfc9a5570336b30b35e2022-12-22T04:38:48ZengElsevierMaterials Today Bio2590-00642022-12-0117100485An all-silk-derived bilayer hydrogel for osteochondral tissue engineeringWeizhou Jiang0Xiuting Xiang1Minkai Song2Jianlin Shen3Zhanjun Shi4Wenhua Huang5Huan Liu6Department of Orthopaedics, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, ChinaDepartment of Biomedical Science, Faculty of Medicine and Health Sciences, University Malaysia Sabah, Kota Kinabalu, 88999, MalaysiaDepartment of Orthopaedics, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, ChinaDepartment of Orthopedics, Affiliated Hospital of Putian University, Putian, 351106, ChinaDepartment of Orthopaedics, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China; Corresponding author.Guangdong Medical Innovation Platform for Translation of 3D Printing Application, The Third Affiliated Hospital of Southern Medical University, Southern Medical University, Guangzhou, 510515, China; Guangdong Engineering Research Center for Translation of Medical 3D Printing Application, Guangdong Provincial Key Laboratory of Medical Biomechanics, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China; Corresponding author. Guangdong Engineering Research Center for Translation of Medical 3D Printing Application, Guangdong Provincial Key Laboratory of Medical Biomechanics, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, China.Department of Orthopedics, The Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou, 646000, China; Corresponding author.Osteochondral repair remains a challenge in clinical practice nowadays despite extensive advances in tissue engineering. The insufficient recruitment of endogenous cells in the early stage and incomplete cell differentiation in the later stage constitute the major difficulty of osteochondral repair. Here, a novel all-silk-derived multifunctional biomaterial platform for osteochondral engineering is reported. The bilayer methacrylated silk fibroin (SilMA) hydrogel was fabricated through stratified photocuring as the basic provisional matrix for tissue regeneration. Platelet-rich plasma (PRP) incorporation promoted the migration and pre-differentiation of the bone marrow mesenchymal stem cells (BMSCs) in the early stage of implantation. The long-term regulation of BMSCs chondrogenesis and osteogenesis was realized by the stratified anchoring of the silk fibroin (SF) microspheres respectively loaded with Kartogenin (KGN) and berberine (BBR) in the hydrogel. The composite hydrogels were further demonstrated to promote BMSCs chondrogenic and osteogenic differentiation under an inflammatory microenvironment and to achieve satisfying cartilage and subchondral bone regeneration with great biocompatibility after 8 weeks of implantation. Since all the components used are readily available and biocompatible and can be efficiently integrated via a simple process, this composite hydrogel scaffold has tremendous potential for clinical use in osteochondral regeneration.http://www.sciencedirect.com/science/article/pii/S2590006422002836OsteoarthritisOsteochondral defectSilk fibroinDrug-loaded microsphereTissue engineering |
| spellingShingle | Weizhou Jiang Xiuting Xiang Minkai Song Jianlin Shen Zhanjun Shi Wenhua Huang Huan Liu An all-silk-derived bilayer hydrogel for osteochondral tissue engineering Materials Today Bio Osteoarthritis Osteochondral defect Silk fibroin Drug-loaded microsphere Tissue engineering |
| title | An all-silk-derived bilayer hydrogel for osteochondral tissue engineering |
| title_full | An all-silk-derived bilayer hydrogel for osteochondral tissue engineering |
| title_fullStr | An all-silk-derived bilayer hydrogel for osteochondral tissue engineering |
| title_full_unstemmed | An all-silk-derived bilayer hydrogel for osteochondral tissue engineering |
| title_short | An all-silk-derived bilayer hydrogel for osteochondral tissue engineering |
| title_sort | all silk derived bilayer hydrogel for osteochondral tissue engineering |
| topic | Osteoarthritis Osteochondral defect Silk fibroin Drug-loaded microsphere Tissue engineering |
| url | http://www.sciencedirect.com/science/article/pii/S2590006422002836 |
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