A Novel Vitronectin Peptide Facilitates Differentiation of Oligodendrocytes from Human Pluripotent Stem Cells (Synthetic ECM for Oligodendrocyte Differentiation)
Differentiation of oligodendrocytes (ODs) presents a challenge in regenerative medicine due to their role in various neurological diseases associated with dysmyelination and demyelination. Here, we designed a peptide derived from vitronectin (VN) using in silico docking simulation and examined its u...
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| Format: | Article |
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MDPI AG
2021-12-01
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| Series: | Biology |
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| Online Access: | https://www.mdpi.com/2079-7737/10/12/1254 |
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| author | Won Ung Park Gyu-Bum Yeon Myeong-Sang Yu Hui-Gwan Goo Su-Hee Hwang Dokyun Na Dae-Sung Kim |
| author_facet | Won Ung Park Gyu-Bum Yeon Myeong-Sang Yu Hui-Gwan Goo Su-Hee Hwang Dokyun Na Dae-Sung Kim |
| author_sort | Won Ung Park |
| collection | DOAJ |
| description | Differentiation of oligodendrocytes (ODs) presents a challenge in regenerative medicine due to their role in various neurological diseases associated with dysmyelination and demyelination. Here, we designed a peptide derived from vitronectin (VN) using in silico docking simulation and examined its use as a synthetic substrate to support the differentiation of ODs derived from human pluripotent stem cells. The designed peptide, named VNP2, promoted OD differentiation induced by the overexpression of SOX10 in OD precursor cells compared with Matrigel and full-length VN. ODs differentiated on VNP2 exhibited greater contact with axon-mimicking nanofibers than those differentiated on Matrigel. Transcriptomic analysis revealed that the genes associated with morphogenesis, cytoskeleton remodeling, and OD differentiation were upregulated in cells grown on VNP2 compared with cells grown on Matrigel. This new synthetic VN-derived peptide can be used to develop a culture environment for efficient OD differentiation. |
| first_indexed | 2024-03-10T04:35:10Z |
| format | Article |
| id | doaj.art-df838b9ed5bc42efa080c52534380655 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T04:35:10Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-df838b9ed5bc42efa080c525343806552023-11-23T03:53:14ZengMDPI AGBiology2079-77372021-12-011012125410.3390/biology10121254A Novel Vitronectin Peptide Facilitates Differentiation of Oligodendrocytes from Human Pluripotent Stem Cells (Synthetic ECM for Oligodendrocyte Differentiation)Won Ung Park0Gyu-Bum Yeon1Myeong-Sang Yu2Hui-Gwan Goo3Su-Hee Hwang4Dokyun Na5Dae-Sung Kim6Department of Biotechnology, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul 02841, KoreaDepartment of Biotechnology, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul 02841, KoreaDepartment of Biomedical Engineering, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 06974, KoreaAMO Life Sciences, 91 Gimpo-daero 1950 beon-gil, Tongjin-eup, Gyeonggi-do 10014, KoreaDepartment of Biomedical Engineering, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 06974, KoreaDepartment of Biomedical Engineering, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 06974, KoreaDepartment of Biotechnology, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul 02841, KoreaDifferentiation of oligodendrocytes (ODs) presents a challenge in regenerative medicine due to their role in various neurological diseases associated with dysmyelination and demyelination. Here, we designed a peptide derived from vitronectin (VN) using in silico docking simulation and examined its use as a synthetic substrate to support the differentiation of ODs derived from human pluripotent stem cells. The designed peptide, named VNP2, promoted OD differentiation induced by the overexpression of SOX10 in OD precursor cells compared with Matrigel and full-length VN. ODs differentiated on VNP2 exhibited greater contact with axon-mimicking nanofibers than those differentiated on Matrigel. Transcriptomic analysis revealed that the genes associated with morphogenesis, cytoskeleton remodeling, and OD differentiation were upregulated in cells grown on VNP2 compared with cells grown on Matrigel. This new synthetic VN-derived peptide can be used to develop a culture environment for efficient OD differentiation.https://www.mdpi.com/2079-7737/10/12/1254oligodendrocytesdifferentiationhPSCsvitronectintranscriptomic analysis |
| spellingShingle | Won Ung Park Gyu-Bum Yeon Myeong-Sang Yu Hui-Gwan Goo Su-Hee Hwang Dokyun Na Dae-Sung Kim A Novel Vitronectin Peptide Facilitates Differentiation of Oligodendrocytes from Human Pluripotent Stem Cells (Synthetic ECM for Oligodendrocyte Differentiation) Biology oligodendrocytes differentiation hPSCs vitronectin transcriptomic analysis |
| title | A Novel Vitronectin Peptide Facilitates Differentiation of Oligodendrocytes from Human Pluripotent Stem Cells (Synthetic ECM for Oligodendrocyte Differentiation) |
| title_full | A Novel Vitronectin Peptide Facilitates Differentiation of Oligodendrocytes from Human Pluripotent Stem Cells (Synthetic ECM for Oligodendrocyte Differentiation) |
| title_fullStr | A Novel Vitronectin Peptide Facilitates Differentiation of Oligodendrocytes from Human Pluripotent Stem Cells (Synthetic ECM for Oligodendrocyte Differentiation) |
| title_full_unstemmed | A Novel Vitronectin Peptide Facilitates Differentiation of Oligodendrocytes from Human Pluripotent Stem Cells (Synthetic ECM for Oligodendrocyte Differentiation) |
| title_short | A Novel Vitronectin Peptide Facilitates Differentiation of Oligodendrocytes from Human Pluripotent Stem Cells (Synthetic ECM for Oligodendrocyte Differentiation) |
| title_sort | novel vitronectin peptide facilitates differentiation of oligodendrocytes from human pluripotent stem cells synthetic ecm for oligodendrocyte differentiation |
| topic | oligodendrocytes differentiation hPSCs vitronectin transcriptomic analysis |
| url | https://www.mdpi.com/2079-7737/10/12/1254 |
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