Improved microbial conversion of de-oiled Jatropha waste into biohydrogen via inoculum pretreatment: process optimization by experimental design approach
In this study various pretreatment methods of sewage sludge inoculum and the statistical process optimization of de-oiled jatropha waste have been reported. Peak hydrogen production rate (HPR) and hydrogen yield (HY) of 0.36 L H2/L-d and 20 mL H2/g Volatile Solid (VS) were obtained when heat shock p...
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| Format: | Article |
| Language: | English |
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Alpha Creation Enterprise
2015-03-01
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| Series: | Biofuel Research Journal |
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| Online Access: | http://www.biofueljournal.com/pdf_8443_a95f999117626ebed0c99d39b4ab8bc1.html |
| _version_ | 1797243609710329856 |
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| author | Gopalakrishnan Kumar Péter Bakonyi Periyasamy Sivagurunathan Nándor Nemestóthy Katalin Bélafi-Bakó Chiu-Yue Lin |
| author_facet | Gopalakrishnan Kumar Péter Bakonyi Periyasamy Sivagurunathan Nándor Nemestóthy Katalin Bélafi-Bakó Chiu-Yue Lin |
| author_sort | Gopalakrishnan Kumar |
| collection | DOAJ |
| description | In this study various pretreatment methods of sewage sludge inoculum and the statistical process optimization of de-oiled jatropha waste have been reported. Peak hydrogen production rate (HPR) and hydrogen yield (HY) of 0.36 L H2/L-d and 20 mL H2/g Volatile Solid (VS) were obtained when heat shock pretreatment (95 oC, 30 min) was employed. Afterwards, an experimental design was applied to find the optimal conditions for H2 production using heat-pretreated seed culture. The optimal substrate concentration, pH and temperature were determined by using response surface methodology as 205 g/L, 6.53 and 55.1 oC, respectively. Under these circumstances, the highest HPR of 1.36 L H2/L-d was predicted. Verification tests proved the reliability of the statistical approach. As a result of the heat pretreatment and fermentation optimization, a significant (~ 4 folds) increase in HPR was achieved. PCR-DGGE results revealed that Clostridium sp. were majorly present under the optimal conditions. |
| first_indexed | 2024-04-24T18:57:50Z |
| format | Article |
| id | doaj.art-df8501a9b0d84fbd8e5e389ff31482fa |
| institution | Directory Open Access Journal |
| issn | 2292-8782 2292-8782 |
| language | English |
| last_indexed | 2024-04-24T18:57:50Z |
| publishDate | 2015-03-01 |
| publisher | Alpha Creation Enterprise |
| record_format | Article |
| series | Biofuel Research Journal |
| spelling | doaj.art-df8501a9b0d84fbd8e5e389ff31482fa2024-03-26T15:13:22ZengAlpha Creation EnterpriseBiofuel Research Journal2292-87822292-87822015-03-012120920148443Improved microbial conversion of de-oiled Jatropha waste into biohydrogen via inoculum pretreatment: process optimization by experimental design approachGopalakrishnan Kumar0Péter Bakonyi1Periyasamy Sivagurunathan2Nándor Nemestóthy3Katalin Bélafi-Bakó4Chiu-Yue Lin5Center for Materials Cycles and Waste Management Research, National Institute for Environmental Studies, Tsukuba, Japan.Research Institute on Bioengineering, Membrane Technology and Energetics, University of Pannonia, Egyetem u. 10, 8200 Veszprém, Hungary.Department of Environmental Engineering, Daegu University, Republic of Korea.Research Institute on Bioengineering, Membrane Technology and Energetics, University of Pannonia, Egyetem u. 10, 8200 Veszprém, Hungary.Research Institute on Bioengineering, Membrane Technology and Energetics, University of Pannonia, Egyetem u. 10, 8200 Veszprém, Hungary.Department of Environmental Engineering and Science, Feng Chia University, 40724 Taichung, Taiwan.In this study various pretreatment methods of sewage sludge inoculum and the statistical process optimization of de-oiled jatropha waste have been reported. Peak hydrogen production rate (HPR) and hydrogen yield (HY) of 0.36 L H2/L-d and 20 mL H2/g Volatile Solid (VS) were obtained when heat shock pretreatment (95 oC, 30 min) was employed. Afterwards, an experimental design was applied to find the optimal conditions for H2 production using heat-pretreated seed culture. The optimal substrate concentration, pH and temperature were determined by using response surface methodology as 205 g/L, 6.53 and 55.1 oC, respectively. Under these circumstances, the highest HPR of 1.36 L H2/L-d was predicted. Verification tests proved the reliability of the statistical approach. As a result of the heat pretreatment and fermentation optimization, a significant (~ 4 folds) increase in HPR was achieved. PCR-DGGE results revealed that Clostridium sp. were majorly present under the optimal conditions.http://www.biofueljournal.com/pdf_8443_a95f999117626ebed0c99d39b4ab8bc1.htmlBiohydrogenDe-oiled Jatropha wasteExperimental designInoculum pretreatmentOptimizationPretreatment |
| spellingShingle | Gopalakrishnan Kumar Péter Bakonyi Periyasamy Sivagurunathan Nándor Nemestóthy Katalin Bélafi-Bakó Chiu-Yue Lin Improved microbial conversion of de-oiled Jatropha waste into biohydrogen via inoculum pretreatment: process optimization by experimental design approach Biofuel Research Journal Biohydrogen De-oiled Jatropha waste Experimental design Inoculum pretreatment Optimization Pretreatment |
| title | Improved microbial conversion of de-oiled Jatropha waste into biohydrogen via inoculum pretreatment: process optimization by experimental design approach |
| title_full | Improved microbial conversion of de-oiled Jatropha waste into biohydrogen via inoculum pretreatment: process optimization by experimental design approach |
| title_fullStr | Improved microbial conversion of de-oiled Jatropha waste into biohydrogen via inoculum pretreatment: process optimization by experimental design approach |
| title_full_unstemmed | Improved microbial conversion of de-oiled Jatropha waste into biohydrogen via inoculum pretreatment: process optimization by experimental design approach |
| title_short | Improved microbial conversion of de-oiled Jatropha waste into biohydrogen via inoculum pretreatment: process optimization by experimental design approach |
| title_sort | improved microbial conversion of de oiled jatropha waste into biohydrogen via inoculum pretreatment process optimization by experimental design approach |
| topic | Biohydrogen De-oiled Jatropha waste Experimental design Inoculum pretreatment Optimization Pretreatment |
| url | http://www.biofueljournal.com/pdf_8443_a95f999117626ebed0c99d39b4ab8bc1.html |
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